Isolation and Structural Identification of Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii

Mori, Hiroharu; Satō, Yoshio; Taketomo, Naoki; Kamiyama, Tomonori; Yoshiyama, Yoshihiro; Meguro, Sachiko; Sato, Hiroko; Kaneko, Tsutomu

doi:10.3168/jds.s0022-0302(97)76138-1

Cited by 82 publications

(51 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that Propionibacterium freudenreichii produces large amounts of tetrahydromenaquinone-9 (MK-9 (4H)) and the precursor 1,4-dyhidroxy-2-naphtoicacid (DHNA) which is a known bifidogenic factor [110][111][112]. In order to improve the production of these metabolites, different laboratory culture protocols that could be applied to an industrial scale have been assayed finding out that DHNA production is markedly influenced by carbon source limitation and the oxygen supply.…”

Section: Nutraceuticals Production: Cla Vitamins Eps and Trehalosementioning

confidence: 99%

“…This property has been demonstrated both in vitro [110,111,178,179], and in vivo [127,[180][181][182] and the bifidogenic growth stimulators (BGS) involved in this effect were identified. The active compounds that were present in supernatants of P. freudenreichii, P. jensenii and P.…”

Section: Gut Microbial Modulationmentioning

confidence: 99%

“…acidipropionici were purified and identified as 2-amino-3-carboxy-1,4-naphtoquinone (ACNQ) [110,178] and 1,4-dihydroxy-2-naphtoic acid (DHNA) a precursor of menaquinone (vitamin K2) biosynthesis [111]. It has been proposed that these compounds serve as electron transfer mediators for NADP regeneration in bifidobacteria [183], thus favoring growth.…”

Section: Gut Microbial Modulationmentioning

confidence: 99%

See 2 more Smart Citations

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Zárate¹

2012

Probiotic in Animals

View full text Add to dashboard Cite

Section: Nutraceuticals Production: Cla Vitamins Eps and Trehalosementioning

confidence: 99%

Section: Gut Microbial Modulationmentioning

confidence: 99%

Section: Gut Microbial Modulationmentioning

confidence: 99%

See 1 more Smart Citation

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Zárate¹

2012

Probiotic in Animals

View full text Add to dashboard Cite

“…They could thus be useful to prevent lactose intolerance. It has also been demonstrated that these bacteria favour the growth of bifidobacteria, both in vitro [20] and in the human intestine [3]. It is noteworthy that some strains of dairy propionibacteria produce bacteriocins.…”

Section: Introductionmentioning

confidence: 99%

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Jan

Leverrier

Proudy

et al. 2002

Lait

View full text Add to dashboard Cite

-Propionibacteria, which are largely used as Swiss-type cheese starters, also constitute promising human probiotics because of their beneficial effects on intestinal bifidobacteria and bacterial enzyme activities. To be efficient, they must survive acid stress imposed within the stomach and the surfactant effects of bile salts. We thus investigated the ability of Propionibacterium freudenreichii (strain Propiofidus ® SI41) to adapt to, and survive these stresses. In an in vivo study, 7 volunteers received Propiofidus ® SI41 in 3 different modes. The probiotic was supplied during 3 periods (i) in classical capsules at a low dose (5 × 10 9 CFU·d -1 ), (ii) in the same capsules at a higher dose (5 × 10 10 CFU·d -1 ), (iii) in acid-resistant capsules at the low dose. In the first case, propionibacteria were recovered above 10 5 CFU·g -1 in only 8 out of 14 faecal samples. In the two following cases, they were enumerated in all faecal samples between 10 5 and 10 7 CFU·g -1 . The recovery of live propionibacteria in faecal samples seemed to be related to a higher content in short chain fatty acids. This study, which clearly showed the role of gastric protection in the survival of propionibacteria, led us to investigate stress adaptation in this strain. Acid tolerance response was evidenced: transient exposure to pH 5 afforded protection towards acid challenge at pH 2. Moreover, we showed that pre-exposure to a moderate bile salts pre-treatment at 0.2 g·L -1 sharply increased its survival against a subsequent lethal challenge (1 g·L -1 ). Important changes in cellular protein synthesis were observed during adaptation using two-dimensional electrophoresis. Global protein synthesis decreased during adaptation treatments. In contrast, specific proteins were overexpressed during acid adaptation and others during bile salts adaptation. Among the up-regulated polypeptides, a carboxyl carrier protein (BCCP) and the recombinase RecR were identified during both stress responses while the universal chaperonins GroEL and GroES, as well as the replicase RepB, were only up-regulated during acid adaptation. These results bring new insights on the tolerance of P. freudenreichii to acid and bile salts, which should be taken into consideration for the development of probiotic preparations. Résumé -Survie et effets bénéfiques des bactéries propioniques dans l'intestin humain : étu-des in vivo et in vitro. Les propionibactéries, qui sont largement utilisées comme levain d'affinage des fromages à pâte pressée cuite, constituent également des probiotiques humains prometteurs du fait de leurs effets bénéfiques sur la flore bifide et les activités enzymatiques bactériennes, au niveau de l'intestin. Pour être efficaces, elles doivent survivre au stress acide subi dans l'estomac et à l'action détergente des sels biliaires. Nous avons donc étudié la capacité de Propionibacterium freudenreichii (souche Propiofidus ® SI41) à s'adapter et à survivre à ces stress. Dans une étude in vivo, 7 volontaires ont reçu Propiofidus ® SI41 selon 3 mo...

show abstract

“…One of the identified active bifidogenic factors is 2-amino-3-carboxy-1,4-napthoquinone produced by a P. freudenreichii strain. Stimulation of growth of Bifidobacterium was observed both after the addition of the compound to the growth medium as well as during the mixed cultivation of the producer strain with bifidobacteria [8]. Several features of Propionibacterium strains suggest that they can be used as probiotic microflora.…”

Section: Introductionmentioning

confidence: 99%

Possibilities for stimulation of Bifidobacterium growth by propionibacteria

Warmińska-Radyko

Laniewska-Moroz

Babuchowski

2002

Lait

View full text Add to dashboard Cite

-Twenty-seven propionic acid bacteria (PAB) strains were studied for their ability to produce metabolites, which stimulated the growth of six Bifidobacterium strains. In addition, the 27 investigated PAB strains were examined for their influence on the growth of four Gram(-) strains belonging to species Escherichia coli and Yersinia enterocolitica, their abilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 4% bile salts as well as their sensitivity against 12 selected antibiotics. All PAB strains stimulated growth of from 3 to 6 of the examined Bifidobacterium strains and simultaneously produced antibacterial metabolites against Gram(-) rods. Fifteen PAB strains inhibited growth of the particular Bifidobacterium strains. Five PAB strains were resistant to acidity surviving for 2 h in pH 2.5 at population level 10 2 -10 6 cfu . mL -1 and 3 strains were resistant to bile salts. Only one PAB strain survived in both the acidic and the bile salt environments. All PAB strains were sensitive to a majority of the antibiotics used in the investigations. Bifidobacterium / Propionibacterium / Gram(-) rod / stimulation / inhibitionRésumé -Stimulation de la croissance de Bifidobacterium par les bactéries propioniques. Vingt sept souches de bactéries propioniques (PAB) ont été examinées pour leur aptitude à produire des métabolites stimulant la croissance de six souches de Bifidobacterium. Par ailleurs, ces 27 souches de PAB ont été examinées pour leur influence sur le développement de quatre souches Gram(-) des espèces Escherichia coli et Yersinia enterocolitica, pour leur capacité de survie dans un environnement à pH 2.0, 2.5, 3.0 et en présence de 4 % de sels biliaires ainsi que pour leur sensibilité à douze antibiotiques sélectionnés. Toutes les souches de PAB ont stimulé le développement de 3 à 6 des souches examinées de Bifidobacterium en produisant en même temps des métabolites antibactériens contre les bacilles Gram(-). Quinze souches de PAB ont eu un effet inhibiteur sur le développement des souches individuelles de Bifidobacterium. Cinq souches de PAB ont résisté à l'acidité et ont 113

show abstract

Isolation and Structural Identification of Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii

Cited by 82 publications

References 13 publications

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Possibilities for stimulation of Bifidobacterium growth by propionibacteria

Contact Info

Product

Resources

About