Isolation and Structural Determination of the Antifouling Diketopiperazines from Marine-Derived &lt;i&gt;Streptomyces praecox&lt;/i&gt; 291-11

Biofilm formation can make significant effects on bacteria habits and biological functions. In this study, diketopiperazines (DKPs) produced by strain of Bacillus amyloliquefaciens Q-426 was found to inhibit biofilm formed in the gas-liquid interface. Four kinds of DKPs were extracted from B. amyloliquefaciens Q-426, and we found that 0.04 mg ml -1 DKPs could obviously inhibit the biofilm formation of the strain. DKPs produced by B. amyloliquefaciens Q-426 made a reduction on extracellular polymeric substance (EPS) components, polysaccharides, proteins, DNAs, etc. Real-time PCR was performed to determine that whether DKPs could make an obvious effect on the expression level for genes related to biofilm formation in the strain. The relative expression level of genes tasA, epsH, epsG and remB which related to proteins, extracellular matrix, and polysaccharides, were downregulated with 0.04 mg ml -1 DKPs, while the expression level of nuclease gene nuc was significantly upregulated. The quantitative results of the mRNA expression level for these genes concerted with the quantitative results on EPS levels. All of the experimental results ultimately indicated that DKPs could inhibit the biofilm formation of the strain B. amyloliquefaciens Q-426.

show abstract

“…(Kumar et al 2012;Wang et al 2010a); and from actinomycetes like Streptomyces spp. (Cho et al 2012;Li et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines

Wang

Yang

Fang

et al. 2016

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…The HPLC chromatograms of these two experiments B9 and E9 were compared in Fig 2. Those of the most active extracts obtained during this study (SE of the large scale culture in TY shown in preliminary assays, SE from B3 and RE from E7) were reported in Fig 3. The compounds previously isolated from this Nocardia strain [16] and known compounds such as cyclo (L-Ala-L-Phe) [21], adenine, adenosine and 1-(5-deoxy-β-D-erythro-pent-4-enofuranosyl) [22] were identified by comparison with a standard and data from literature (Figs 2A, 2B and 3). The main differences between the production of metabolites by the strain in the RE of B9 and E9 are due to the presence of an unidentified compound with a r.t. of 19 min and of a diketopiperazine cyclo-(L-Ala-L-Phe) which were produced in large amount in bioreactor B9 but not in Erlenmeyer flask E9 (Fig 2A).…”

Section: Comparison Of Hplc Profiles Of the Most Active Extractsmentioning

confidence: 99%

Optimization of cytotoxic activity of Nocardia sp culture broths using a design of experiments

et al. 2020

View full text Add to dashboard Cite

In the context of research for new cytotoxic compounds, obtaining bioactive molecules from renewable sources remain a big challenge. Microorganisms and more specifically Actinobacteria from original sources are well known for their biotechnological potential and are hotspots for the discovery of new bioactive compounds. The strain DP94 studied here had shown an interesting cytotoxic activity of its culture broth (HaCaT: IC 50 = 8.0 ± 1.5 μg/mL; B16: IC 50 = 4.6 ± 1.8 μg/mL), which could not been explained by the compounds isolated in a previous work. The increase of the cytotoxic activity of extracts was investigated, based on a Taguchi L9 orthogonal array design, after DP94 culture in TY medium using two different vessels (bioreactor or Erlenmeyer flasks). Various culture parameters such as temperature, pH and inoculum ratio (%) were studied. For experiments conducted in a bioreactor, stirring speed was included as an additional parameter. Significant differences in the cytotoxic activities of different extracts on B16 melanoma cancer cell lines, highlighted the influence of culture temperature on the production of cytotoxic compound(s) using a bioreactor. A culture in Erlenmeyer flasks was also performed and afforded an increase of the production of the active compounds. The best conditions for the highest cytotoxicity (IC 50 on B16: 6 ± 0.5 μg/mL) and the highest yield (202.0 mg/L) were identified as: pH 6, temperature 37˚C and 5% inoculum.

show abstract

“…Alteromonas sp.KNS-16, a lobocompactol-resistant strain, 46 and it was found that the production titres of this compound were increased 10-fold. The authors demonstrated that the amount of challenger inoculum is an important factor to consider when performing co-culture experiments.…”

Section: Co-culturingmentioning

confidence: 99%

“…Although all levels of challenger inoculum resulted in production, it was recognised that an optimum inoculum strength existed for the production of lobocompactol (22) with an inoculum of 10 5 cells of challenger resulting in peak production levels (25% greater than when a 10 6 inoculum was used, and approximately 300% greater than 10 4 innoculum was used). 46 The much studied model actinomycete S. coelicolor has also been subjected to co-culturing studies, and it has been demonstrated that Myxococcus xanthus induces the overproduction of actinorhodin (23) by 20-fold. 47 In elegant research on S. coelicolor by Dorrestein and coworkers, employing nanospray desorption electrospray ionisation (NanoDESI) and matrix-assisted desorption ionisation time of flight (MALDI-TOF) imaging spectroscopy, the researchers were able to capture a global overview of the chemical cross talk of this model organism as it interacted with five other actinomycetes.…”

Section: Co-culturingmentioning

confidence: 99%

Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products

et al. 2016

View full text Add to dashboard Cite

Over the centuries, microbial secondary metabolites have played a central role in the treatment of human diseases and have revolutionised the pharmaceutical industry. With the increasing number of sequenced microbial genomes revealing a plethora of novel biosynthetic genes, natural product drug discovery is entering an exciting second golden age. Here, we provide a concise overview as an introductory guide to the main methods employed to unlock or up-regulate these so called 'cryptic', 'silent' and 'orphan' gene clusters, and increase the production of the encoded natural product. With a predominant focus on bacterial natural products we will discuss the importance of the bioinformatics approach for genome mining, the use of first different and simple culturing techniques and then the application of genetic engineering to unlock the microbial treasure trove.

show abstract

Isolation and Structural Determination of the Antifouling Diketopiperazines from Marine-Derived <i>Streptomyces praecox</i> 291-11

Cited by 40 publications

References 47 publications

Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines

Inhibition of biofilm in Bacillus amyloliquefaciens Q-426 by diketopiperazines

Optimization of cytotoxic activity of Nocardia sp culture broths using a design of experiments

Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products

Contact Info

Product

Resources

About