Isolation and structural characterization of enkephalins in the brain of the Rhynchobdellid leech <i>Theromyzon tessulatum</i>

Salzet, Michel; Bulet, Philippe; Verger-Bocquet, Martine; Malecha, Jean

doi:10.1016/0014-5793(94)01358-8

Cited by 26 publications

(7 citation statements)

References 31 publications

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“…Salzet et al (1993b) isolated and characterized structurally a vasopressin-related peptide, Lysconopressin, in the central nervous system of a leech, Erpobdella octoculata; Lys-conopressin was first isolated from a gastropod mollusc, Conus geographus (Cruz et al 1987). Furthermore, Salzet and colleagues isolated and identified chemically Met-and Leu-enkephalins in a leech, Theromyzon tessulatum (Salzet et al 1995a) and angiotensin II amide in E. octoculata (Salzet et al 1995b). An oxytocin-related peptide, annectocin, was isolated from an earthworm, Eisenia foetida (Oumi et al 1994).…”

Section: Introductionmentioning

confidence: 96%

Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Ukena

Oumi

Morishita

et al. 1997

Cell and Tissue Research

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The quantity and localization of Eisenia tetradecapeptide which was isolated from the gut of the earthworm Eisenia foetida were examined in tissues of the same species by enzyme-linked immunosorbent assay and immunohistochemistry. Analysis by enzyme-linked immunosorbent assay showed that Eisenia-tetradecapeptide-like immunoreactivity was present in both the central nervous system (cerebral ganglion, subesophageal ganglion, ventral ganglia, and ventral nerve cord) and the gut (esophagus, crop, gizzard, and intestine). The central nervous system contained a higher amount of Eisenia-tetradecapeptide-like immunoreactivity (1.3 pmol/mg wet weight) than the gut (0.2-0.6 pmol/mg wet weight). Eisenia-tetradecapeptide-like immunoreactivity was scarcely detected in the body-wall muscle, nephridia, and sexual organs (testis, ovary, seminal vesicle, and ovisac). Immunohistochemical analysis demonstrated that intense Eisenia-tetradecapeptide-like immunopositive cells and nerve fibers were present in the central nervous system. Immunoreactivity was found in the epithelial cells lining the esophagus and in the submucous plexus in various parts of the gut. Thus, the present study suggests that Eisenia tetradecapeptide is a neuropeptide and/or peptide hormone present in both the central nervous system and the gut of the earthworm and that its role involves the regulation of gut motility.

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Section: Introductionmentioning

confidence: 96%

Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Ukena

Oumi

Morishita

et al. 1997

Cell and Tissue Research

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“…In annelids, many immunohistochemical studies with antibodies against vertebrate peptides have reported that structurally related peptides exist in annelids (Alumets et al 1979;Rzasa et al 1984;Li and Calabrese 1985;Verger-Bocquet et al 1987;Salzet et al 1992Salzet et al , 1993b, but there are very few studies on endogenous annelid peptides (Salzet et al 1996a;Ukena et al 1997), except for FMRFamide (Kuhlman et al 1985;Evans and Calabrese 1989;Krajniak and Greenberg 1992;Salzet et al 1993b). By means of immunological assays, several peptides have been isolated from leeches which display a high degree of similarity to vertebrate peptides: lysineconopressin (Salzet et al 1993a), methionine-enkephalin and leucine-enkephalin (Salzet et al 1995a), angiotensin II amide (Salzet et al 1995b), and α-neo-endorphin-related peptide (Salzet et al 1996b). We also have isolated several bioactive peptides from the three classes of Annelida by bioassay using isolated gut preparations (Matsushima et al 1993;Oumi et al 1994Oumi et al , 1995Takahashi et al 1994;Ukena et al 1995Ukena et al , 1996.…”

Section: Introductionmentioning

confidence: 98%

Localization of leech excitatory peptide, a member of the GGNG peptides, in the central nervous system of a leech ( Whitmania pigra ) by immunohistochemistry and in situ hybridization

Nagahama

Ukena

Oumi

et al. 1999

Cell and Tissue Research

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We have recently isolated a myoactive peptide, called leech excitatory peptide, belonging to the GGNG peptide family from two species of leeches, Hirudo nipponia and Whitmania pigra. Immunohistochemistry and in situ hybridization were employed to localize leech excitatory peptide-like peptide(s) and its gene expression in the central nervous system of W. pigra. A pair of neuronal somata were stained by both immunohistochemistry and in situ hybridization in the supraesophageal, subesophageal, and segmental ganglia. In addition, several other neurons showed positive signals by either immunohistochemistry or in situ hybridization in these ganglia. An immunoreactive fiber was observed to run in the anterior root of segmental ganglion 6, which is known to send axons to the sexual organs, though we failed to detect immunoreactivity in possible target tissues. Antiserum specificity was established by enzyme-linked immunosorbent assay using different leech excitatory peptide-related peptides. Leech excitatory peptide elicited muscular contraction of isolated preparations of penis and intestine at concentrations of 10(-8 )M. These results suggest that leech excitatory peptide is a neuropeptide modulating neuromuscular transmission in multiple systems, including regulation of reproductive behavior.

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“…This peptide has also been reported to potentiate spontaneous contractions of gut tissue [10]. Salzet et al [11] isolated and structurally characterized methionine‐ and leucine‐enkephalins from the brain of the rhynchobdellid leech Theromyzon tessulatum by means of ELISA and dot immunobinding assays. However, the biological activity of these peptides in leeches has not been reported.…”

Section: Introductionmentioning

confidence: 99%

The leech excitatory peptide, a member of the GGNG peptide family: isolation and comparison with the earthworm GGNG peptides

et al. 1997

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A member of the GGNG peptide family was isolated from Hirudo nipponia (leech). GGNG peptides had only been isolated previously from earthworms. The C-terminus structure of the leech peptide, LEP (leech excitatory peptide), was -Gly-Gly-Asn-amide, while that of the earthworm peptides, EEP (earthworm excitatory peptide), was -Gly-Gly-Asn-Gly. LEP exerted 1000-fold more potent activities on leech gut than did EEP-2. On the other hand, EEP-2 was 1000-fold more potent than LEP on the crop-gizzard of the earthworm. Analog peptides of LEP and EEP-2 were synthesized, and the myoactive potency of each analog on the leech and earthworm tissues was compared.

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Isolation and structural characterization of enkephalins in the brain of the Rhynchobdellid leech Theromyzon tessulatum

Cited by 26 publications

References 31 publications

Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Localization of leech excitatory peptide, a member of the GGNG peptides, in the central nervous system of a leech ( Whitmania pigra ) by immunohistochemistry and in situ hybridization

The leech excitatory peptide, a member of the GGNG peptide family: isolation and comparison with the earthworm GGNG peptides

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