Isolation and Some Properties of β-Galactosidase Producing Bacteria

Ikura, Yoko; Horikoshi, Koki

doi:10.1080/00021369.1979.10863410

Cited by 7 publications

(7 citation statements)

References 2 publications

(4 reference statements)

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“…Strain C-125 was isolated from a soil sample by using an alkaline culture medium in 1970 (42), and it was kept in the form of spores on a plate until 1977. This strain was characterized as a ␤-galactosidase producer and identified as a member of the genus Bacillus in 1977 (20) after being subcultured several times for experiments (21). Thereafter, the strain was kept in the form of spores on a plate again until 1983.…”

Section: Resultsmentioning

confidence: 99%

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Identification and Distribution of New Insertion Sequences in the Genome of Alkaliphilic Bacillus halodurans C-125

et al. 2001

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Section: Resultsmentioning

confidence: 99%

“…Alkaliphilic Bacillus halodurans strain C-125 (JCM9153) was isolated in 1970 and characterized as a ␤-galactosidase producer (21) and xylanase producer (17,18). It is the most thoroughly characterized strain, physiologically, biochemically, and genetically, among those in our collection of alkaliphilic Bacillus isolates (19).…”

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confidence: 99%

Identification and Distribution of New Insertion Sequences in the Genome of Alkaliphilic Bacillus halodurans C-125

et al. 2001

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“…Morphological properties and taxonomic characteristics of the bacterium were investigated by the method described in "Bergey's Manual of Systematic Bacteriology" 15) and "Horikoshi's Reports." [16][17][18] Abbreviations: CA, cholic acid; COCA, chenodeoxycholic acid; UOCA, ursodeoxycholic acid; OHCA, dehydrocholic acid; 12KCDCA, 12-ketochenodeoxycholic acid (3a,7a-dihydroxy-12-keto-5fJ-cholanic acid); 7KOOCA, 7-ketodeoxycholic acid (3a,12a-di-hydroxy-7-keto-5fJ-cholanic acid); 7,12DKLCA, 7,12-diketolithocholic acid (7,12-diketo-3a-hydroxy-5fJ-cholanic acid); 7KLCA, 7-ketolithocholic acid (3a-hydroxy-7-keto-5fJ-cholanic acid); 12-epi-CA, 3a,7a, 12fJ-trihydroxy-5fJ-cholanic acid; NTG, N-methyl-N'-nitro-N-nitrosoguanidine; HSDH, hydroxysteroid dehydrogenase.…”

Section: Methodsmentioning

confidence: 99%

Oxidation of 3-, 7-, and 12-hydroxyl Groups of Cholic Acid by an AlkalophilicBacillussp.

Kimura¹,

Okamura²,

Kawaide³

1994

Bioscience, Biotechnology, and Biochemistry

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“…strain C‐125 grows well at high pH values above 9.5 and maintains an intracellular pH around 8.6 when grown at pH 10.5 [1]. This strain also can grow at neutral pH values near pH 7, if sufficient sodium ion (1–2%) was supplied to the culture medium [2]. Many alkaliphilic Bacillus strains, including C‐125, produce commercial alkaline enzymes such as xylanases [3], proteases and amylases [4] under alkaline conditions.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of the gene encoding RNA polymerase Î± subunit from alkaliphilicBacillussp. strain C-125

Nakasone

Takaki

Takami

et al. 1998

FEMS Microbiology Letters

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The rpoA gene, encoding the alpha subunit of RNA polymerase, was isolated from alkaliphilic Bacillus sp. strain C-125 by the PCR method. A 3-kb HindIII fragment containing the complete rpoA gene was cloned and sequenced. The alpha subunit gene was found to encode a protein consisting of 314 amino acid residues with a molecular mass of 34,805 Da. Compared with the amino acid sequences of other known eubacterial RNA polymerase alpha subunits, the gene has 84% identity to that of B. subtilis, while showing 48% and 47% identity to that of Streptomyces coelicolor and Escherichia coli, respectively. Six conserved regions, which are observed in the case of other eubacteria, were found in the RNA polymerase alpha subunit of this strain. Five of them are located in the N-terminal domain involved in assembly of the core enzyme, while one is located in the C-terminal domain, which interacts with several transcriptional factors and a specific DNA element. By means of recombinant plasmids, a hexahistidine-tagged derivative of the RNA polymerase alpha subunit of strain C-125 and two deletion derivatives (C- and N-terminal domains) of this protein were overexpressed in E. coli cells and purified to near homogeneity.

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Isolation and Some Properties of β-Galactosidase Producing Bacteria

Cited by 7 publications

References 2 publications

Identification and Distribution of New Insertion Sequences in the Genome of Alkaliphilic Bacillus halodurans C-125

Identification and Distribution of New Insertion Sequences in the Genome of Alkaliphilic Bacillus halodurans C-125

Oxidation of 3-, 7-, and 12-hydroxyl Groups of Cholic Acid by an AlkalophilicBacillussp.

Cloning and expression of the gene encoding RNA polymerase Î± subunit from alkaliphilicBacillussp. strain C-125

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