Isolation and some properties of exohemagglutinin from the culture medium of Bacteroides gingivalis 381

Abstract

Exohemagglutinin was found in the culture medium of Bacteroides gingivalis 381. Exohemagglutinin was purified 3,150-fold from culture fluid by ultracentrifugation followed by gel filtration on Sepharose CL-4B and by affinity chromatography on argmine-agarose. Examination of the final preparation of exohemagglutinin by biochemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the isolated

Surface properties and ultrastructure of Porphyromonas gingivalis W50 and pleiotropic mutants

“…It seems likely therefore that the haemagglutinin receptor is associated with the outer membrane proteins of P. gingwalis. Haemagglutinin is inhibited at low concentrations of arginine (7,17) and it has been suggested that arginine ftmctions as an active residue between the haeterial receptor and erythrocytes (3).…”

“…P. gingivalis, for example, characteristically produces adhesins which enable attachment to erythrocytes (haemagglutinin) and to other bacteria! ceils (7) and adheres both to buccal and crevicular epithelial cells probably by means of fimbriae (3,, 8). In the present study the ultrastructure and other surface properties, such as the haemagglutinin activity and the hydrophobicity, were compared between virulent and avirulent strains.…”

Possibility of Bacteroides gingivalis Hemagglutinin Possessing Protease Activity Revealed by Inhibition Studies

“…Bacteroides gingivalis is able to agglutinate erythrocytes (4,5,9) and this property is thought to be one of the reasons for the pathogenicity of the microbe. Recently, Inoshita et al (3) and Okuda et al (6) independently reported unique properties of a hemagglutinin (HA) partially purified from the culture medium of B. gingivalis. Inoshita et al (3) reported that the activity of the HA, unlike lectins, is not inhibited by sugars but inhibited by L-arginine and L-arginine-containing peptides.…”

“…Recently, Inoshita et al (3) and Okuda et al (6) independently reported unique properties of a hemagglutinin (HA) partially purified from the culture medium of B. gingivalis. Inoshita et al (3) reported that the activity of the HA, unlike lectins, is not inhibited by sugars but inhibited by L-arginine and L-arginine-containing peptides. Okuda et al (6) obtained similar findings : although sugars were not inhibitory, L-arginine and L-lysine were inhibitory.…”

Restriction fragment length polymorphism analysis of two hemagglutinin loci, serotyping and agglutinating activity of Porphyromonas gingivalis isolates

“…P. gingivalis is a gram-negative, strictly anaerobic rod exhibiting a large number of potential virulence factors, including hemagglutinins, that may be implicated in initiation of periodontal diseases and tissue destruction (23,24). P. gingivalis hemagglutinins have been studied both at the protein (3,6,8,17,18,27,29,(32)(33)(34) and at the molecular genetic levels (20,25,37,41). Progulske-Fox et al (37) were the first to report the cloning of a hemagglutinin gene, defined as hagA, from P. gingivalis strain 381.…”