Isolation and some properties of Thiobacillus ferrooxidans strains with differing levels of mercury resistance from natural environments

Takeuchi, Fumiaki; Iwahori, Kenji; Kamimura, Kazuo; Sugio, Tsuyoshi

doi:10.1016/s1389-1723(99)80215-1

Cited by 26 publications

(13 citation statements)

References 16 publications

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“…ferrooxidans has been characterized. It is apparent that purified cytochrome-c oxidase from resistant strains is more tolerant to increased levels Hg(II), which, due to its importance in Fe(II)-oxidizing activity, likely contributes to the resistant strain's increased growth rate (Takeuchi et al, 1999).…”

Section: Mercurymentioning

confidence: 99%

Growth in sulfidic mineral environments: metal resistance mechanisms in acidophilic micro-organisms

et al. 2003

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Acidophilic micro-organisms inhabit some of the most metal-rich environments known, including both natural and man-made ecosystems, and as such are ideal model systems for study of microbial metal resistance. Although metal resistance systems have been studied in neutrophilic micro-organisms, it is only in recent years that attention has been placed on metal resistance in acidophiles. The five metal resistance mechanisms identified in neutrophiles are also present in acidophiles, in some cases utilizing homologous proteins, but in many cases the degree of resistance is greater in acidophiles. This review summarizes the knowledge of acidophile metal resistance and presents preliminary in silico studies on a few known metal resistance systems in the sequenced acidophile genomes.

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Section: Mercurymentioning

confidence: 99%

Growth in sulfidic mineral environments: metal resistance mechanisms in acidophilic micro-organisms

et al. 2003

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“…It has been known that A. ferrooxidans cells are in general resistant to many heavy metals including iron, copper, zinc, and nickel, but sensitive to mercury, silver, molybdenum, and tungsten. [4][5][6][7][8][9][10] We have reported the growth inhibition of A. ferrooxidans cells by mercury, silver, molybdenum, and tungsten and clariˆed inhibition sites for these toxic metals. [7][8][9][10][11] Among these toxic metals mercury is highly toxic for almost all organisms because they have a strong a‹nity for thiol groups in proteins.…”

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confidence: 99%

Volatilization of Mercury by an Iron Oxidation Enzyme System in a Highly Mercury-resistantAcidithiobacillus ferrooxidansStrain MON-1

Sugio¹,

Fujii²,

Takeuchi³

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…One volume of crude extract was added into MRAS solution and incubated at room temperature for various interval durationof 30, 60, 90, 120 and 150 minutes. Mercury reductase activity was measured spectrophotometrically at 340 nm (Takeuchi et al, 1999). A one unit of mercury reductase activity was defined as one molar of oxidized NADH 2 produced per total cell per minute in the assay condition (Zeroual et al, 2003).…”

Section: Mercury Reductase Assay System (Mras)mentioning

confidence: 99%

MERCURY REDUCTASE ACTIVITY OF AN INDIGENOUS MERCURY RESISTANT BACTERIAL ISOLATE (Bacillus sp. S1) FROM KALIMAS-SURABAYA AS A POTENTIAL REDUCING AGENT FOR MERCURIAL ION (HG2+)

Zulaika

Sembiring

2015

KLS

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Mercury reductase activity and Hg 2+ lowering capacity of a Mercury Resistant Bacteria (MRB) (Bacillus sp. S1) which was isolated from Kalimas River of Surabaya, Indonesia were studied. The activity was determined by Mercury Reductase Assay System (MRAS) in a solution mixture contained 50 mM PBS (pH ± 7.0), 0.5 mM EDTA, 200 µM MgSO4, 0.1% (v/v) ß-merchaptoethanol, 200 µM NADH2 and 25 mg/L HgCl 2 and one volume of crude extract incubated at room temperature for various interval period of time. Mercury reductase activity was measured spectrophotometrically at 340 nm. One unit of reductase activity was defined as one molar of oxidized NADH 2 produced per total cell per minute. Results of study showed that, the isolate could resist the concentration of HgCl 2 up to 11 mg/L. At 30 minute incubation period at room temperature, the highest mercury reductase activity and the Hg 2+ lowering capacity was found to be 0.006 unit/109cell and 1.48 mg/L/109cell/minute, respectively with the reduction efficiency of Hg 2+ to Hg 0 of 0.18% per minute. Therefore, it can be concluded that the Bacillus sp. S1 isolate could be assumed to be exellent mercury bioremediation agent since it was found to be highly mercury resistant and very efficient to reduce cationic mercury (Hg 2+ ) to elemental mercury (Hg 0 ).

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Isolation and some properties of Thiobacillus ferrooxidans strains with differing levels of mercury resistance from natural environments

Cited by 26 publications

References 16 publications

Growth in sulfidic mineral environments: metal resistance mechanisms in acidophilic micro-organisms

Growth in sulfidic mineral environments: metal resistance mechanisms in acidophilic micro-organisms

Volatilization of Mercury by an Iron Oxidation Enzyme System in a Highly Mercury-resistantAcidithiobacillus ferrooxidansStrain MON-1

MERCURY REDUCTASE ACTIVITY OF AN INDIGENOUS MERCURY RESISTANT BACTERIAL ISOLATE (Bacillus sp. S1) FROM KALIMAS-SURABAYA AS A POTENTIAL REDUCING AGENT FOR MERCURIAL ION (HG2+)

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