Isolation and sequencing of swine carbonic anhydrase VI, an enzyme expressed in the swine kidney

Abstract

BackgroundCarbonic anhydrase VI (CA-VI) is produced by the salivary gland and is secreted into the saliva. Although CA-VI is found in the epithelial cells of distal straight tubule of swine kidneys, the exact function of CA-VI in the kidneys remains unclear.ResultsCA-VI was located in the epithelial cells of distal straight tubule of swine kidneys.A full-length cDNA clone of CA-VI was generated from the swine parotid gland by reverse transcription polymerase chain reaction, using degenerate primers designed based on conserved regions of the same locus in human and bovine tissues.The cDNA sequence was 1348 base pairs long and was predicted to encode a 317 amino acid polypeptide with a putative signal peptide of 17 amino acids. The deduced amino acid sequence of mature CA-VI was most similar (77.4%) to that of human CA-VI. CA-VI expression was confirmed in both normal and nephritic kidneys, as well as parotid. As the primers used in this study spanned two exons, the influence of genomic DNA was not detected. The expression of CA-VI was demonstrated in both normal and nephritic kidneys, and mRNA of CA-VI in the normal kidneys which was the normalised to an endogenous β–actin was 0.098 ± 0.047, while it was significantly lower in the diseased kidneys (0.012 ± 0.007). The level of CA-VI mRNA in normal kidneys was 19-fold lower than that of the parotid gland (1.887).ConclusionsThe localisation of CA-VI indicates that it may play a specialised role in the kidney.

Gene transcripts expressed in equine white blood cells are potential biomarkers of extracorporeal shock wave therapy

“…51 It is also produced in the nasal gland 52 and the kidney. 53 It was reported that urinary CAH6 might represent a biomarker for kidney disease in pigs, particularly for disorders affecting distal straight tubules. 54 Its role following ESWT administration is not clear.…”

“…This enzyme is abundantly found in salivary glands, and saliva has been implicated in taste and gastrointestinal dysfunctions, tooth erosion, and caries 51 . It is also produced in the nasal gland 52 and the kidney 53 . It was reported that urinary CAH6 might represent a biomarker for kidney disease in pigs, particularly for disorders affecting distal straight tubules 54 .…”