Isolation and sequence of a genomic clone encoding the basic form of pathogenesis-related protein 1 fromNicotiana tabacum

Payne, George B.; Middlesteadt, Wayne; Desai, Nalini; Williams, Shericca; Dincher, Sandra; Carnes, Michael G.; Ryals, John

doi:10.1007/bf00036973

Cited by 44 publications

(33 citation statements)

References 4 publications

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“…They exhibited differential antifungal activity in vitro and in vivo (leaf disc assay) toward P. infestam. The protein with the higher antifungal activity, P14c, showed only low sequence similarity (65%) to the initially described P14 (Lucas et al, 1985) but high similarity (94%) to the basic tobacco PR-1 predicted from a cDNA clone (Cornelissen et al, 1987) and from genomic DNA clones (Payne et al, 1989;Eyal et al, 1992). This putative basic PR protein, named PR-lg, was then isolated from tobacco leaves reacting hypersensitively to tobacco mosaic virus, along with the three well-known acidic proteins PR-la, PR-lb, and PR-lc.…”

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confidence: 88%

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Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Niderman¹,

Genetet²,

Bruyère³

et al. 1995

Plant Physiol.

389

193

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Three distinct basic 14-kD proteins, P14a, P14b, and P14c, were isolated from tomato (Lycopersicon esculentum Mill. cv Baby) leaves infected with Phytophthora infestans. They exhibited antifungal activity against P. infestam both in vitro (inhibition of zoospore germination) and in vivo with a tomato leaf disc assay (decrease in infected leaf surface). Serological cross-reactions and amino acid sequence comparisons showed that the three proteins are members of the PR-1 group of pathogenesis-related (PR) proteins. P14a and P14b showed high similarity to a previously characterized P14, whereas P14c was found to be very similar to a putative basic-type PR-1 from tobacco predicted from isolated DNA clones. This protein, named PR-1 g, was purified from virus-infected tobacco (Nicotiana tabacum Samsun NN) leaves and characterized by amino acid microsequencing, along with the well-known acidic tobacco PR-la, PR-lb, and PR-lc. l h e various tomato and tobacco PR-1 proteins were compared for their biological activity and found to display differential fungicidal activity against P. infestam in both the in vitro and in vivo assays, the most efficient being the newly characterized tomato P14c and tobacco PR-1 g.

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mentioning

confidence: 88%

“…Protein PR-lg was predicted from sequence data (Cornelissen et al, 1987;Payne et al, 1989;13yal et al, 1992) to be a basic protein. Therefore, we used a procedure similar to the one that allowed the isolation of c ther basic tobacco PR proteins (Geoffroy et al, 1990;Heitz et al, 1994b).…”

Section: Purification Of Tobacco Pr-1 Proteinsmentioning

confidence: 99%

Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Niderman¹,

Genetet²,

Bruyère³

et al. 1995

Plant Physiol.

389

193

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“…The PR-1-related cDNA was isolated by cross-hybridization to tobacco PR-1 acidic and basic cDNAs at low stringency (Payne et al, 1988b(Payne et al, , 1989. The sequence of the longest of three identical clones is shown in Figure 5A.…”

Section: Biological Responses Of Arabidopsis To Chemical Lmmunizationmentioning

confidence: 99%

“…The Arabidopsis PR-1 cDNA was isolated by screening the cDNA library at low stringency with a mixture of both the PR-la (Payne et al, 1988b) and PR-lbasic (Payne et al, 1989) cDNAs from tobacco as probes. The cDNA library was plated at 5000 plaque-forming units per plate, and filter lifts were taken with nitrocellulose filters (Ausubel et al, 1987).…”

Section: Pr-5 Proteinmentioning

confidence: 99%

Acquired resistance in Arabidopsis.

et al. 1992

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Acquired resistance is an important component of the complex disease resistance mechanism in plants, which can result from either pathogen infection or treatment with\synthetic, resistance-inducing compounds. In this study, Arabidopsis, a tractable genetic system, is shown to develop resistance to a bacterial and a fungal pathogen following 2,6-dichloroisonicotinic acid (INA) treatment. Three proteins that accumulated to high levels in the apoplast in response to INA treatment were purified and characterized. Expression of the genes corresponding to these proteins was induced by INA, pathogen infection, and salicylic acid, the latter being a putative endogenous signal for acquired resistance.Arabidopsis should serve as a genetic model for studies of this type of immune response in plants.

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“…PR-1 genes are expressed due to various external stimuli like pathogens, injury, chemical elicitors, hormones, UV light (9) and salicylic acid. PR-1 proteins contain a hydrophobic N-terminal region of 30 amino acids that probably function as a signal peptide for the translocation to the endoplasmic reticulum (38). The FORESTs database has sequences (EGSBRT3122B04.g and EGBMCL1291G04.g) similar to C. annum PR-1 and a precursor protein of maize, respectively (Table 5).…”

Section: Pathogenesis-related Proteins (Pr-proteins)mentioning

confidence: 99%

Eucalyptus ESTs involved in mechanisms against plant pathogens and environmental stresses

et al. 2010

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RESUMOEucalypt plantation has high economical importance in Brazil; however, it has been attacked by various pathogens under different environmental stress conditions. Disease resistance and survival under unfavorable environmental conditions have revealed that the eucalypt has developed highly efficient defense systems. t o o t h e r a l r e a d y k n o w n m o l e c u l e s , s u c h a s p l a n t -s i g n a l i n g molecules, phytoalexins, lignin biosynthesis pathways, PR-proteins an d p u tati v e g en es co r resp o n d i n g to e n zymes i n v o l v ed in t h e detox ification of reactiv e o xyg en species, were id entified. We a l s o p r e s e n t g e n e r a l c o n s i d e r a t i o n s a bout the mechanisms of Eucalyptus defense against biotic and abiotic stresses. These data are of extreme importance for future eucalypt breeding programs aimed at developing plants with enhanced resistance against pathogens and environmental stresses.por agentes estressantes, identificou-se os grupos de seqüências de cDNA similares a outras moléculas já conhecidas, como moléculas de plantas-sinalizadoras, fitoalexinas e biossíntese de vias de lignina, proteínas-RP e supostos genes correspondendo às enzimas envolv idas na detoxificação de moléculas de ox igênio reativo. Apresentamos também, considerações gerais sobre os mecanismos de defesa do Eucalipto contra o estresse biótico e abiótico.

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Isolation and sequence of a genomic clone encoding the basic form of pathogenesis-related protein 1 fromNicotiana tabacum

Cited by 44 publications

References 4 publications

Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Acquired resistance in Arabidopsis.

Eucalyptus ESTs involved in mechanisms against plant pathogens and environmental stresses

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