Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize

Kirihara, Julie Anderson; Petri, Jean Bernhard; Messing, Joachim

doi:10.1016/0378-1119(88)90053-4

Cited by 104 publications

(52 citation statements)

References 42 publications

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“…The zein genomic clone pZ10-H3 (Kirihara et al, 1988) was digested with XbaI and then blunt-ended by fill-in reaction with Klenow. The reaction was incubated with NcoI, which releases the 10-kD zein coding sequence.…”

Section: T-dna Vector Construction and Rice Transformationmentioning

confidence: 99%

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Hamada

Ishiyama

Sakulsingharoj

et al. 2003

THE PLANT CELL ONLINE

107

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Prolamine and glutelin RNAs are localized to two subdomains of the cortical endoplasmic reticulum (ER), the protein body ER and the cisternal ER, in developing rice seeds. The addition of nearly full-length prolamine sequences at the 3 untranslated region of a reporter RNA redirects its localization from the cisternal ER to the protein body ER. Deletion analysis of prolamine RNA sequences indicates the presence of two partially redundant cis elements required for protein body ER targeting. The addition of glutelin 3 untranslated region to protein body ER cis sequences, however, redirects RNA localization to the cisternal ER. These results indicate that there are at least two regulated RNA transport pathways as well as a constitutive pathway to the cortical ER.

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Section: T-dna Vector Construction and Rice Transformationmentioning

confidence: 99%

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Hamada

Ishiyama

Sakulsingharoj

et al. 2003

THE PLANT CELL ONLINE

107

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“…Two cloned γ zeins with a molecular weight of 16 and 27 kDa are very high in proline (25%; Prat et al, 1987). The two δ zeins with a molecular weight of 10 and 18 kDa are very high in methionine (23% and 28%, respectively; Kirihara et al, 1988;Swarup et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Amplicons of maize zein genes are conserved within genic but expanded and constricted in intergenic regions

Llaca

Messing

1998

The Plant Journal

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“…The tissue-and development-specific 10-kD zein promoter (Kirihara et al, 1988) was used for expression of the SacB gene. The promoter and a CaMV 3' end (Pietrzak et al, 1986) were ligated to the SacB coding regions, using unique restriction enzyme sites included in PCR primers.…”

Section: Construction Of Endosperm-specific Sacb Expression Vectorsmentioning

confidence: 99%

“…Three expression vectors were constructed containing the B. nmyloliquefaciens SacB gene directed by a 10-kD zein seed storage promoter (Kirihara et al, 1988); these are represented in Figure 1. Targeting the bacterial protein to vacuoles in maize endosperm was accomplished by transformation with expression vectors containing secretion and targeting sequences fused to the mature SacB coding sequence.…”

Section: Construction Of Sacb Expression Vectorsmentioning

confidence: 99%

Fructan Accumulation and Sucrose Metabolism in Transgenic Maize Endosperm Expressing a Bacillus amyloliquefaciens SacB Gene

et al. 1996

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Over 40,000 species of plants accumulate fructan, p-2-1-and P-2-6-linked polymers of fructose as a storage reserve. Due to their high fructose content, severa1 commercial applications for fructans have been proposed. However, plants that accumulate these polymers are not agronomically suited for large-scale cultivation or processing. This study describes the transformation of a Bacillus amyloliguefaciens SacB gene into maize (Zea mays 1.) callus by particle bombardment. Tissue-specific expression and targeting of the SacB protein to endosperm vacuoles resulted in stable accumulation of high-molecular-weight fructan in mature seeds. Accumulation of fructan in the vacuole had no detectable effect on kernel development or germination. Fructan levels were found to be approximately 9-fold higher in sh, mutants compared to wild-type maize kernels. In contrast to vacuole-targeted expression, starch synthesis and endosperm development in mature seeds containing a cytosolically expressed SacB gene were severely affected. The data demonstrate that hexose resulting from cytosolic SacB activity was not utilized for starch synthesis. Transgenic seeds containing a chimeric SacB gene provide further evidence that the dominant pathway for starch synthesis in maize endosperm is through uridine diphosphoglucose catalyzed by the enzyme sucrose synthase.

show abstract

Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize

Cited by 104 publications

References 42 publications

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Dual Regulated RNA Transport Pathways to the Cortical Region in Developing Rice Endosperm

Amplicons of maize zein genes are conserved within genic but expanded and constricted in intergenic regions

Fructan Accumulation and Sucrose Metabolism in Transgenic Maize Endosperm Expressing a Bacillus amyloliquefaciens SacB Gene

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