Isolation and sequence analysis of napin seed specific promoter from Iranian Rapeseed (Brassica napus L.)

Sohrabi, Maryam; Zebarjadi, Alireza; Najaphy, Abdollah; Kahrizi, Danial

doi:10.1016/j.gene.2015.03.040

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…This resulted in the production of pBI121-proEmb5:GUS. For negative control, the 54 bp of 35S minimal promoter (Sohrabi et al 2015), which is not expressed in a plant, was used. pBI121, which has the 35S:GUS expression cassette, was used as the positive control in the subsequent Arabidopsis transformation studies.…”

Section: Construction Of Proemb5:gus Plant Expression Vector and The mentioning

confidence: 99%

“…After confirmation by colony PCR and sequencing, the individual DNA fragments were subsequently ligated to pBI121 to generate pBI121-proEmbA-G-GUS constructs. In addition, the individual conserved region of the ProEmb5 was added by overlapping PCR to upstream of the 54 bp 35S minimal promoter (Sohrabi et al 2015). These fragments were subsequently ligated to the pBI121 vector, replacing of the original 35S promoter to result in the production of a series of GUS expression constructs as indicated in Fig.…”

Section: Construction Of Proemb5:gus Plant Expression Vector and The mentioning

confidence: 99%

“…For example, by expressing the promoter region of the peanut 8A4R19G1 gene in the model plants Arabidopsis and tobacco, this groundnut seed promoter (GSP) showed seed-specific activity (Sunkara et al 2014). Similarly, the Napin promoter of Brassica napus has been confirmed to drive seed-specific expression of GUS, and it has been widely used to express many genes in dicotyledonous plants (Sohrabi et al 2015). A few seed-specific promoters from maize have also been isolated recently.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the Promoter of Emb5 from Zea mays Identifies a Region of 523 bp Responsible for Its Embryo-Specific Activity

Yang

Wang

et al. 2020

Plant Mol Biol Rep

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The maize Emb5 is an abscisic acid–responsive gene which is specifically expressed in the late embryo during seed maturity. To further dissect and identify the elements specific for its embryo expression pattern, we investigated the activity of the − 1653 bp upstream of the “full-length” promoter region of this gene in transgenic Arabidopsis plants. We first confirmed that the “full-length” promoter could indeed drive the expression of β-glucuronidase reporter gene (GUS) in the transgenic Arabidopsis seed embryo. Subsequently, DNA fragments of ~ 500 bp in length were generated after a series of progressive deletions from positions − 1653 bp to − 1 bp relative to the transcriptional initiation site. These fragments were fused with GUS and introduced into Arabidopsis. Measurement of the GUS activity in the immature seeds isolated from the transgenic plants revealed that the region between positions − 523 bp and − 1 bp, namely ProEm-D, is absolutely required and sufficient for the temporal and embryo-specific expression of GUS with an activity comparable with the full-length Emb5 promoter in Arabidopsis. Therefore, our results clearly demonstrated that the 523 bp ProEm-D can replace the − 1653 bp Emb5 promoter to drive embryo-specific expression in Arabidopsis seed. Because of its small size and strong embryo-specific activity, it could become the promoter of choice in metabolic pathway engineering to transfer multiple genes for the production of valuable pharmaceutical products in seeds, such as polyunsaturated fatty acids found in fish oils, or pro-vitamin A where at least three transgenes are required to assemble the entire metabolic pathways.

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Section: Construction Of Proemb5:gus Plant Expression Vector and The mentioning

confidence: 99%

Section: Construction Of Proemb5:gus Plant Expression Vector and The mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of the Promoter of Emb5 from Zea mays Identifies a Region of 523 bp Responsible for Its Embryo-Specific Activity

Yang

Wang

et al. 2020

Plant Mol Biol Rep

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“…A few tissue-specific promoters have been isolated and identified in crops, including rice [8,10,[15][16][17][18][19][20][21][22], maize [23][24][25], soybean [26][27][28][29][30], wheat [31,32], as well as some other species [33][34][35][36][37][38][39][40][41][42][43][44]. Among these tissue-specific promoters, the most attention has been paid to seed-specific promoters derived from the promoters of storage-protein-related genes, such as soybean [29,30], rapeseed [36], sunflower [37], and peanut [38]. Endospermspecific, fruit-specific, and seed-coat-specific promoters have also been identified in rice and maize [45][46][47], tomato [33], and Arabidopsis [34], respectively.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean

Zhang

Guan

et al. 2023

Genes

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Promoters play a crucial role in controlling the spatial and temporal expression of genes at transcriptional levels in the process of higher plant growth and development. The spatial, efficient, and correct regulation of exogenous genes expression, as desired, is the key point in plant genetic engineering research. Constitutive promoters widely used in plant genetic transformation are limited because, sometimes, they may cause potential negative effects. This issue can be solved, to a certain extent, by using tissue-specific promoters. Compared with constitutive promoters, a few tissue-specific promoters have been isolated and applied. In this study, based on the transcriptome data, a total of 288 tissue-specific genes were collected, expressed in seven tissues, including the leaves, stems, flowers, pods, seeds, roots, and nodules of soybean (Glycine max). KEGG pathway enrichment analysis was carried out, and 52 metabolites were annotated. A total of 12 tissue-specific genes were selected via the transcription expression level and validated through real-time quantitative PCR, of which 10 genes showed tissue-specific expression. The 3-kb 5′ upstream regions of ten genes were obtained as putative promoters. Further analysis showed that all the 10 promoters contained many tissue-specific cis-elements. These results demonstrate that high-throughput transcriptional data can be used as effective tools, providing a guide for high-throughput novel tissue-specific promoter discovery.

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“…Oil seeds are the second global food resources among which Brassica napus L. is the third annual oil seed in the world (1)(2)(3)(4). Oilseed rapes are the world's third most important source of vegetable oils after palm and soybean (5)(6)(7)(8)(9). Low temperatures are important environmental factors limiting plant distribution, survival, and crop yields worldwide.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous responses of photosystem II and soluble proteins of rapeseed to cold acclimation

Arminian

Bidgoli

2019

Cell Mol Biol (Noisy-le-grand)

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This experiment was conducted to assess the quantitative and qualitative changes in soluble proteins as well as some chlorophyll fluorescence parameters in the leaves of a winter canola (Brassica napus L., cv. Licord) under continuous low temperature. Over the experiment, seedlings were initially grown at 15/10 °C (d/n). At fourth fully expanded leafy stage (day 30), a part of the plants were transferred to 4/2°C for 4 weeks. Plants were sampled for protein extraction from leaves in which chlorophyll fluorescence parameters (F o , F v , F m , F v/ F o, F m/ F o, F v /F m , F o´, F V´, F m´ and some other calculated) were also measured. The results showed a clear increase in soluble proteins quantity caused by cold treatment. The enhancements appeared abruptly following the cold exposure to 4°C and lasted. The electrophoretic protein patterns showed changes in the intensity of some polypeptides, besides, induction a new probable protein weighing 47-kW in response to cold treatment. Cold-triggered reduction in maximum quantum yield of PSII (F v /F m) was connected especially with drastic decreasing F v and F m. Interestingly, high quantitative amounts of soluble proteins along with induction of the new probable polypeptide induced at cold temperature, were attributed to low deduction of maximum quantum yield of PSII. Additionally, more imperative chlorophyll fluorescence parameters changed e.g. qP, NPQ, qL, Y(II) or ф PSII etc at light. Nowadays, radar charts or spider plots are the most sophisticated multivariate statistical tools representing physiological responses of plants to abiotic stress conditions or even morphophysiological studies of plants. In rapeseed many researches performed by applying the radar charts for low temperature stresses and interpreted their effects more advancely than common statistical tools. We observed a good representation of the chl fluorescence parameters fluctuations using radar plots. Overall, cold-induced soluble proteins accumulated after longer cold-acclimation, can contribute in photosynthetic apparatus protection against low-temperature damages.

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Isolation and sequence analysis of napin seed specific promoter from Iranian Rapeseed (Brassica napus L.)

Cited by 7 publications

References 26 publications

Analysis of the Promoter of Emb5 from Zea mays Identifies a Region of 523 bp Responsible for Its Embryo-Specific Activity

Analysis of the Promoter of Emb5 from Zea mays Identifies a Region of 523 bp Responsible for Its Embryo-Specific Activity

Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean

Simultaneous responses of photosystem II and soluble proteins of rapeseed to cold acclimation

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