ISOLATION AND SEQUENCE ANALYSIS OF A PIGMENT-DISPERSING HORMONE FROM EYESTALKS OF THE CRAB,<i>CANCER MAGISTER</i>

Kleinholz, L. H.; Rao, K. Ranga; Riehm, John P.; Tarr, George E.; Johnson, Linda L.; Norton, Sheila E.

doi:10.2307/1541386

Cited by 34 publications

(9 citation statements)

References 30 publications

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“…Uca pugilator NSELINSILGLPKVMNDA-NH 2 (Rao et al ., 1985) Cancer magister NSELINSILGLPKVMNDA-NH 2 (Kleinholz et al ., 1986) Callinectes sapidus NSELINSILGLPKVMNDA-NH 2 (Mohrherr et al ., 1990) Orconectes immunis NSELINSILGLPKVMNEA-NH 2 (Rao and Riehm, 1993) Penaeus aztecus NSELINSLLGIPKVMNDA-NH 2 (Rao and Riehm, 1993) Pandalus borealis NSGMINSILGIPRVMTEA-NH 2 (Rao and Riehm, 1993) Insect (PDF)…”

Section: Crustacean (Pdh)mentioning

confidence: 99%

Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

Singaravel¹,

Fujisawa²,

Hisada³

et al. 2003

Zoological Science

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-Pigment-dispersing factors (PDFs) are octadeca-peptides widely distributed in insect optic lobes and brain. In this study, we have purified PDF and determined its amino acid sequence in the cricket Gryllus bimaculatus . Its primary structure was NSEIINSLLGLPKVLNDA-NH 2 , homologous to other PDH family members so far reported. When injected into the optic lobe of experimentally blinded adult male crickets, Gryllus -PDF induced phase shifts in their activity rhythms in a phase dependent and dose dependent manner. The resulted phase response curve (PRC) showed delays during the late subjective night to early subjective day and advances during the mid subjective day to mid subjective night. The PRC was different in shape from those for light, serotonin and temperature. These results suggest that PDF plays a role in phase regulation of the circadian clock through a separate pathway from those of other known phase regulating agents.

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Section: Crustacean (Pdh)mentioning

confidence: 99%

Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

Singaravel¹,

Fujisawa²,

Hisada³

et al. 2003

Zoological Science

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“…The major form of this hormone from Pandalus borealis (Fernlund, 1976), identified as an octadecapeptide (DRPH, a-PDH), was shown to elicit pigment dispersion in chromatophores and light-adaptational eye pigment movements (Kleinholz, 1975;Josefsson, 1983). The major form of PDH from brachyuran decapods such as Uca pugilator , Cancer magister (Kleinholz et al, 1986), and Callinectes sapidus (Mohrherr et al, 1990), has been identified as p-PDH.…”

Section: Discussionmentioning

confidence: 99%

“…and designated as P-PDK has been identified as the major form in the eyestalks of the fiddler crab, Uca pugilator . An identical P-PDH (Asn-Ser-Glu-Leu-Ile-Asn-Ser-Ile-Leu-Gly-Leu-Pro-Lys-Val-Met-Asn-Asp-Ala-NH2) is present in other crab species: Cancer magister (Kleinholz et al, 1986) and Callinectes sapidus (Mohrherr et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Primary Structure and Relative Potency of an Analog of β‐PDH (Pigment‐Dispersing Hormone) From the Crayfish Procambarus clarkii

McCALLUM

Rao

Riehm

et al. 1991

Pigment Cell Research

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A pigment-dispersing hormone (PDH) from eyestalks of the crayfish Procambarus clarkii was purified by gel filtration, cation-exchange chromatography, partition chromatography, and reversed-phase HPLC. Based on automated sequencing and by the identical chromatographic behavior of the native PDH and the synthetic amidated form of the deduced sequence, the primary structure of Procambarus PDH has been established as: Asn-Ser-Glu-Leu-Ile-Asn-Ser-Ile-Leu-Gly-Leu-Pro-Lys-Val-Met-Asn-Glu-Ala- NH2. This peptide differs from beta-PDH of the fiddler crab Uca pugilator at a single position, Glu17 in place of Asp17. Because of this substitution, Procambarus PDH was 4 to 7-fold less potent than beta-PDH in causing pigment dispersion in the erythrophores, leucophores, and melanophores of Uca. In contrast, Procambarus PDH was 4-fold more potent than beta-PDH in eliciting pigment dispersion in the erythrophores of Procambarus. These peptides displayed less marked differences in potency in triggering leucophore pigment dispersion and light-adaptational distal eye pigment movement in Procambarus. These findings indicate that the structural requirements for PDH-receptor interactions vary with the species and with the target cell type within a given species.

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“…Brachyuran crabs show the opposite response to Caridea, and their dark chromatophore pigments aggregate in the absence of a pigment‐dispersing hormone (PDH) (Lambert and Fingerman, ; Kulkarni and Fingerman, ). This octadecapeptide exists as two isoforms, α‐PDH (Asn‐Ser‐Gly‐Met‐Ile‐Asn‐Ser‐Ile‐Leu‐Gly‐Ile‐Pro‐Arg‐Val‐Met‐Thr‐Glu‐Ala‐NH 2 ; Fernlund, ,; Josefsson, ), and β‐PDH (Asn‐Ser‐Glu‐Leu‐Ile‐Asn‐Ser‐Ile‐Leu‐Gly‐Leu‐Pro‐Lys‐Val‐Met‐Asn‐Asp‐Ala‐NH 2 ; Rao, ; Kleinholtz et al., ; Mohrherr et al., ). Although a role for PDH in pigment dispersion is well known in crabs (Oguro, ; Castrucci and Mendes, ; Rao, ) and isopods (Fingerman, ; Oguro, ; Castrucci and Mendes, ), it is not clear whether PDH induces pigment dispersion in caridean shrimp chromatophores (Tuma et al., ) or if the dispersion mechanism is triggered simply by RPCH uncoupling from its plasma membrane receptor.…”

Section: Introductionmentioning

confidence: 99%

Pigment Translocation in Caridean Shrimp Chromatophores: Receptor Type, Signal Transduction, Second Messengers, and Cross Talk Among Multiple Signaling Cascades

et al. 2016

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Pigment aggregation in shrimp chromatophores is triggered by red pigment concentrating hormone (RPCH), a neurosecretory peptide whose plasma membrane receptor may be a G-protein coupled receptor (GPCR). While RPCH binding activates the Ca /cGMP signaling cascades, a role for cyclic AMP (cAMP) in pigment aggregation is obscure, as are the steps governing Ca release from the smooth endoplasmic reticulum (SER). A role for the antagonistic neuropeptide, pigment dispersing homone (α-PDH) is also unclear. In red, ovarian chromatophores from the freshwater shrimp Macrobrachium olfersi, we show that a G-protein antagonist (AntPG) strongly inhibits RPCH-triggered pigment aggregation, suggesting that RPCH binds to a GPCR, activating an inhibitory G-protein. Decreasing cAMP levels may cue pigment aggregation, since cytosolic cAMP titers, when augmented by cholera toxin, forskolin or vinpocentine, completely or partially impair pigment aggregation. Triggering opposing Ca /cGMP and cAMP cascades by simultaneous perfusion with lipid-soluble cyclic nucleotide analogs induces a "tug-of-war" response, pigments aggregating in some chromatosomes with unpredictable, oscillatory movements in others. Inhibition of cAMP-dependent protein kinase accelerates aggregation and reduces dispersion velocities, suggesting a role in phosphorylation events, possibly regulating SER Ca release and pigment aggregation. The second messengers IP and cADPR do not stimulate SER Ca release. α-PDH does not sustain pigment dispersion, suggesting that pigment translocation in caridean chromatophores may be regulated solely by RPCH, since PDH is not required. We propose a working hypothesis to further unravel key steps in the mechanisms of pigment translocation within crustacean chromatophores that have remained obscure for nearly a century.

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ISOLATION AND SEQUENCE ANALYSIS OF A PIGMENT-DISPERSING HORMONE FROM EYESTALKS OF THE CRAB,CANCER MAGISTER

Cited by 34 publications

References 30 publications

Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

Primary Structure and Relative Potency of an Analog of β‐PDH (Pigment‐Dispersing Hormone) From the Crayfish Procambarus clarkii

Pigment Translocation in Caridean Shrimp Chromatophores: Receptor Type, Signal Transduction, Second Messengers, and Cross Talk Among Multiple Signaling Cascades

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