Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Liu, Yao-Chia; Ni, Nian-Tong; Chang, Ju-Chun; Li, Yi-Hsuan; Lee, Mi Rong; Nai, Yu‐Shin

doi:10.3791/62882

Cited by 7 publications

(11 citation statements)

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“…To avoid environmental pollution, economical pest management using EPFs was accepted as effective alternative agents to chemical control (Bamisile et al 2021). EPFs isolated from the soil of certain region are more efficient in controlling local pests in the same area (Liu et al 2021). Meyling (2007) accepted the insect bait technique which used in this study by G. mellonella larvae as the most successful method for isolating Page 5 of 8 Ahmed et al Egyptian Journal of Biological Pest Control (2022) 32:67 EPF from soil.…”

Section: Discussionmentioning

confidence: 99%

Identification and evaluation of isolated entomopathogenic fungus from Egyptian soil against the black cutworm larvae of Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)

Ahmed

Khalil

Sahab

2022

Egypt J Biol Pest Control

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Background The black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), is considered as one of the most destructive pests of many field crops in Egypt. Entomopathogenic fungi (EPF) have long been accepted as relatively safe alternatives to chemical insecticides. In the past decades, many researchers have compared the effective local isolates of EPF to the formulated product of the same fungus and reported that local isolates were more efficient than formulations when used against target insects. Results The present study discussed the pathogenic activity of local isolate after scientific characterization. Two insect species [Galleria mellonella L. and Agrotis ipsilon (Hufn.)] were used in this evaluation. The fungus was isolated from soil samples by trapping method, and fourth-instar larvae of Galleria were used to evaluate the pathogenicity of the isolate. The isolate was identified as Beauveria bassiana. Different levels of the fungus development depending on the test temperatures (20, 25, 30, 35 and 40 °C) were tested. Optimal growth of the isolate fungus was recorded at 25 °C. The corrected mortality rate for fourth-instar larvae of G. mellonella treated with the isolate spores at 6.4 × 105 spores/ml was (96%) after 10 days, while the mortality of last instar larvae of A. ipsilon was (48 and 100%) at the concentrations of 9.2 × 104 and 2.9 × 106 spores/ml, respectively, after 14 days compared to (24%) in the control. Both pupation and the sex ratio in adults were affected at different levels according to the concentration. The effect was also very clear in the increased rates of malformation in adults. Conclusion The EPF, B. bassiana, isolated from the soil, was highly effective against G. mellonella larvae in storage and A. ipsilon larvae in soil; therefore, it could be recommended as an alternative control agent for chemical pesticides.

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Section: Discussionmentioning

confidence: 99%

Identification and evaluation of isolated entomopathogenic fungus from Egyptian soil against the black cutworm larvae of Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)

Ahmed

Khalil

Sahab

2022

Egypt J Biol Pest Control

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“…Through the ECN analysis, the potential fungal isolates, which harbor more tolerance to environmental stresses, would be selected for application in the field [ 14 , 15 ]. It is notable that all the tested fungal isolates showed higher thermotolerance compared to the results of the previous studies obtained with B. bassiana and M. anisopliae [ 14 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, six fungal isolates were isolated from diseased F. taiwana and subjected to the selection system based on Liu et al, 2021. The pathogenicity screening by using mealworm ( T. molitor ) was applied to evaluate the insect-killing activity of these fungal isolates [ 14 , 15 ]. The mortality of the six fungal isolates against mealworms was 100%, indicating the insecticidal potential of the isolates.…”

Section: Discussionmentioning

confidence: 99%

“…Totally, six fungal isolates (two fungal isolates from last stage of larvae and four from emerging adults) were purified using single-spore method and stored in SDA slants at 4 °C for further studies. Pathogenicity screening was based on the Tenebrio molitor -mediated system followed by the method from Tettor-Barsch et al and others [ 11 , 12 , 13 , 14 , 15 ]. The mortality of treated T. molitor larvae was observed and recorded daily for 10 days.…”

Section: Methodsmentioning

confidence: 99%

“…At each time point, 5 mm of agar block was detached from the center of the colony and put in 1 mL 0.03% Silwet TM in a 1.5 mL tube. The sample was vortexed at 3000 rpm at room temperature for 15 min and the number of conidia was calculated by a hemocytometer under light microscopy [ 14 , 15 ]. Each treatment was replicated thrice.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of the Potential Entomopathogenic Fungi Purpureocillium lilacinum and Fusarium verticillioides for Biological Control of Forcipomyia taiwana (Shiraki)

Liao

et al. 2022

JoF

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Forcipomyia taiwana (Diptera: Ceratopogonidae) is a nuisance blood-sucking pest to humans in Taiwan. An F. taiwana bite causes itching and redness and usually causes serious harassment to human outdoor activity. In terms of F. taiwana control, chemical pesticides are ineffective. Therefore, other efforts are needed. Fungal mycosis in the larvae, pupae, and emerging F. taiwana adults was found during the rearing of F. taiwana. In this study, six fungal isolates were isolated from infected cadavers and subjected to molecular identification. In addition, their biocontrol potential was evaluated against different life stages of F. taiwana. Based on the pathogenicity screening, two fungal isolates, NCHU-NPUST-175 and -178, which caused higher mortality on the fourth instar larvae of F. taiwana, were selected for virulence tests against different life stages of F. taiwana larvae. The results of the phylogenetic analysis indicated that the NCHU-NPUST-175 and -178 belonged to Purpureocillium lilacinum and Fusarium verticillioides, respectively. Bioassay against different life stages of F. taiwana with different spore concentrations (5 × 105 to 5 × 107 conidia/mL) revealed a dose-dependent effect on larvae for both fungal isolates, while only 38% and 50% mortality was found in highest concentration (5 × 107 conidia/mL) at fourth instar larvae by Pl-NCHU-NPUST-175 and Fv-NCHU-NPUST-178, respectively. Moreover, reductions in egg-hatching rate and adult emergence rate were found, when the last stage of F. taiwana was inoculated with both fungal isolates, indicating the ovicidal potential and the impact of entomopathogenic fungi on the development of F. taiwana. In conclusion, Pl-NCHU-NPUST-175 and Fv-NCHU-NPUST-178 showed larvicidal activity, ovicidal activity, and impact on adult emergence on F. taiwana.

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Potential of a combination of entomopathogenic fungal strains and a non-ionic surfactant to control the fall armyworm (Spodoptera frugiperda)

Tseng

Yang

et al. 2022

Journal of Asia-Pacific Entomology

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Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Cited by 7 publications

References 0 publications

Identification and evaluation of isolated entomopathogenic fungus from Egyptian soil against the black cutworm larvae of Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)

Identification and evaluation of isolated entomopathogenic fungus from Egyptian soil against the black cutworm larvae of Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)

Evaluation of the Potential Entomopathogenic Fungi Purpureocillium lilacinum and Fusarium verticillioides for Biological Control of Forcipomyia taiwana (Shiraki)

Potential of a combination of entomopathogenic fungal strains and a non-ionic surfactant to control the fall armyworm (Spodoptera frugiperda)

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