Isolation and screening of potassium solubilizing bacteria from saxicolous habitat and their impact on tomato growth in different soil types

Muthuraja, Raji; Muthukumar, Thangavelu

doi:10.1007/s00203-021-02284-9

Cited by 37 publications

(23 citation statements)

References 55 publications

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“…Adding half of K requirements for faba bean in a mineral form with 40 kg of HA and PSB led to 14% and 19% increases in the seed and straw yield compared to the full mineral fertilization without bacterial inoculation. In another study, Raji and Thangavelu (2021) isolated fifteen culturable saxicolous (rock-dwelling) bacterial isolates with varied K solubilizing ability from two sites. Of these, four potential K solubilizer isolates were identified as Bacillus subtilis, Bacillus cereus, Bacillus licheniformis and Burkholderia cenocepacia by 16S rRNA gene sequencing.…”

Section: Types Of Biofertilizersmentioning

confidence: 99%

Biofertilizers: An ecofriendly technology for nutrient recycling and environmental sustainability

Kumar

Diksha

Sindhu

et al. 2022

Current Research in Microbial Sciences

212

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Highlights Agriculture plays an important role in a country's economy. In modern intensive agricultural practices, chemical fertilizers and pesticides are applied on large scale to increase crop production in order to meet the nutritional requirements of the ever-increasing world population. However, rapid urbanization with shrinking agricultural lands, dramatic change in climatic conditions and extensive use of agrochemicals in agricultural practices has been found to cause environmental disturbances and public health hazards affecting food security and sustainability in agriculture. Besides this, agriculture soils are continuously losing their quality and physical properties as well as their chemical (imbalance of nutrients) and biological health due to indiscriminate use of agrochemicals. Plant-associated microbes with their plant growth- promoting traits have enormous potential to solve these challenges and play a crucial role in enhancing plant biomass and crop yield under greenhouse and field conditions. The beneficial mechanisms of plant growth improvement include enhanced availability of nutrients (i.e., N, P, K, Zn and S), phytohormone modulation, biocontrol of phytopathogens and amelioration of biotic and abiotic stresses. This plant-microbe interplay is indispensable for sustainable agriculture and these microbes may perform essential role as an ecological engineer to reduce the use of chemical fertilizers. Various steps involved for production of solid-based or liquid biofertilizer formulation include inoculum preparation, addition of cell protectants such as glycerol, lactose, starch, a good carrier material, proper packaging and best delivery methods. In addition, recent developments of formulation include entrapment/microencapsulation, nano-immobilization of microbial bioinoculants and biofilm-based biofertilizers. Thus, inoculation with beneficial microbes has emerged as an innovative eco-friendly technology to feed global population with available resources. This review critically examines the current state-of-art on use of microbial strains as biofertilizers in different crop systems for sustainable agriculture and in maintaining soil fertility and enhancing crop productivity. It is believed that acquisition of advanced knowledge of plant-PGPR interactions, bioengineering of microbial communities to improve the performance of biofertilizers under field conditions, will help in devising strategies for sustainable, environment-friendly and climate smart agricultural technologies to deliver short and long terms solutions for improving crop productivity to feed the world in a more sustainable manner.

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Section: Types Of Biofertilizersmentioning

confidence: 99%

Biofertilizers: An ecofriendly technology for nutrient recycling and environmental sustainability

Kumar

Diksha

Sindhu

et al. 2022

Current Research in Microbial Sciences

212

View full text Add to dashboard Cite

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“…Among these 110 bacterial isolates, only 13 isolates showed K solubilizing ability by forming a halo zone on Aleksandrov’s agar media ( Figure 1 ). In a previous study, the isolation of 15 K-solubilizing rhizobacteria (KSR) from soil samples collected from hilly areas depending on their capability to solubilize mica as insoluble K source was reported [ 2 ]. Studies have also shown that different species of KSB exist in the soil and play a vital role in the K cycle [ 42 , 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…Serial dilution and pour plating technique was used for isolating the K solubilizing bacteria, followed by incubating the plates at 10 ± 0.2 °C in an inverted position for five days. The colonies that formed hollow zones on the mica amended medium were considered positive for mineral K solubilization and were further purified by repeated streaking on Aleksandrov’s medium, then preserved at 4 °C for further studies [ 2 ]. The standard strain Bacillus mucilaginosus used as a control was obtained from the Department of Soil Science and Agricultural Chemistry, VN Marathwada Agriculture University, Parbhani, Maharashtra, India.…”

Section: Methodsmentioning

confidence: 99%

“…Potassium (K) is one of the main macronutrients and the most abundant cation in higher plants [ 1 ], with significant absorption that plays a vital role in metabolic activity, activation of ~80 enzymes, starch synthesis, sugar degradation, photosynthesis, disease resistance, etc. [ 2 , 3 ]. There are four K forms found in the soil, namely, soil minerals, non-exchangeable, exchangeable, and water soluble [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…An array of such bacteria, such as Bacillus sp. [ 2 , 14 ], Pseudomonas sp. [ 25 ], Paenibacillus sp.…”

Section: Introductionmentioning

confidence: 99%

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Psychrotolerant Mesorhizobium sp. Isolated from Temperate and Cold Desert Regions Solubilizes Potassium and Produces Multiple Plant Growth Promoting Metabolites

et al. 2021

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Soil potassium (K) supplement depends intensively on the application of chemical fertilizers, which have substantial harmful environmental effects. However, some bacteria can act as inoculants by converting unavailable and insoluble K forms into plant-accessible forms. Such bacteria are an eco-friendly approach for enhancing plant K absorption and consequently reducing utilization of chemical fertilization. Therefore, the present research was undertaken to isolate, screen, and characterize the K solubilizing bacteria (KSB) from the rhizosphere soils of northern India. Overall, 110 strains were isolated, but only 13 isolates showed significant K solubilizing ability by forming a halo zone on solid media. They were further screened for K solubilizing activity at 0 °C, 1 °C, 3 °C, 5 °C, 7 °C, 15 °C, and 20 °C for 5, 10, and 20 days. All the bacterial isolates showed mineral K solubilization activity at these different temperatures. However, the content of K solubilization increased with the upsurge in temperature and period of incubation. The isolate KSB (Grz) showed the highest K solubilization index of 462.28% after 48 h of incubation at 20 °C. The maximum of 23.38 µg K/mL broth was solubilized by the isolate KSB (Grz) at 20 °C after 20 days of incubation. Based on morphological, biochemical, and molecular characterization (through the 16S rDNA approach), the isolate KSB (Grz) was identified as Mesorhizobium sp. The majority of the strains produced HCN and ammonia. The maximum indole acetic acid (IAA) (31.54 µM/mL) and cellulase (390 µM/mL) were produced by the isolate KSB (Grz). In contrast, the highest protease (525.12 µM/mL) and chitinase (5.20 µM/mL) activities were shown by standard strain Bacillus mucilaginosus and KSB (Gmr) isolate, respectively.

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Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

Issifu,

Naitchede,

Ateka

et al. 2023

Agrosystems Geosci & Env

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Plant growth‐promoting rhizobacteria enhances nutritional availability during root colonization. Pseudomonas is the most popular bioinoculant due to its synergistic effects on plant development. This study evaluated the impact of four Pseudomonas strain substrate inoculations on tomato phenology, yield, and selected phytochemical components relevant to human health. The bacterial isolates have been characterized morphologically, biochemically, physiologically, and phylogenetically. Tomato plants on cocopeat substrate were inoculated individually with a bacterial suspension (optical density [OD]600 2.0). Controls included nitrogen–phosphate–potassium (NPK) fertilizer and tap water. The greenhouse experiment was laid out in a completely randomized design with five replications. Data were taken 30, 60, 90, and 120 days after inoculation. All four bacterial isolates could fix nitrogen, solubilize phosphate, and potassium and synthesize indole acetic acid in vitro. The 16S rRNA of the strains BF1P2‐6, BF2P1‐2, BF2P5‐1, and BF4P2‐5 were identified as Pseudomonas putida Y22, Pseudomonas taiwanensis GDLB‐32, Pseudomonas monteilii Bil‐NOR3_15, and Pseudomonas fluorescens Y5, respectively. The strains grew best at pH 7, NaCl 0.0%, and 35°C. Inoculated tomato plants tested over a period of 4 months in a greenhouse showed a significant increase in growth, leaf variables, and yield attributes. Pseudomonas‐inoculated plants were the highest in tannins (46.96 mg/100 g), followed by lycopene (45.57 mg/100 g) and Carotenoids (31.87 mg/100 g). The antioxidant activity of tomato fruit extracts showed the potential for free radicals scavenging activity against 2, 2‐diphenyl‐1‐picrylhydrazyl. Conditions such as pH and electrical conductivity for the cocopeat substrate were within acceptable limits. Bacterial strains and NPK increased cocopeat nitrogen, phosphate, and potassium bioavailability. This study reveals that Pseudomonas strains improve plant growth, yield, and health‐related phytochemical substances in tomato fruits, which could broaden their application in fruit and vegetable crops.

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Isolation and screening of potassium solubilizing bacteria from saxicolous habitat and their impact on tomato growth in different soil types

Cited by 37 publications

References 55 publications

Biofertilizers: An ecofriendly technology for nutrient recycling and environmental sustainability

Biofertilizers: An ecofriendly technology for nutrient recycling and environmental sustainability

Psychrotolerant Mesorhizobium sp. Isolated from Temperate and Cold Desert Regions Solubilizes Potassium and Produces Multiple Plant Growth Promoting Metabolites

Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

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