Isolation and screening of antibiotic producing actinomycetes from rhizosphere and agricultural soils

Muleta, Atsede; Assefa, Fassil

doi:10.5897/ajb2017.16080

Cited by 12 publications

(10 citation statements)

References 26 publications

(29 reference statements)

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“…Soil samples were collected from ten different spots in the area of study. Samples were collected from 10 cm down the soil surface with the aid of sterile spatula in a sterile universal container and taken to the laboratory for culture (Muleta & Assefa, 2018).…”

Section: Sample Collectionmentioning

confidence: 99%

“…Thus, it is indispensable in the management of infectious agents especially now that drug resistant forms of these agents are on the increase. Since man's hope for success in the fight against infectious agents is a continuous process, microorganisms being known to produce numerous secondary useful metabolites will continue to be investigated in different habitats including sewage contaminated soil for new antibiotics (Muleta & Assefa, 2018;Rafiq et al, 2018;Ladan, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic Activity of a Newly Discovered Aspergillus Species Isolated from Sewage Dump Site

M. O.,

C. O.,

C. U.

et al. 2024

African Journal of Biology and Medical Research

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Infectious agents causing diseases are becoming resistant to drugs produced to manage them. This has continued to spur scientific investigations for newer and better antibiotics to aid and/or replace existing ones. Soil samples were collected from sewage dump sites with the sole aim of isolating and screening fungi species for antibacterial substances using the cultural method. A newly discovered fungi species of Aspergillus was used to produce crude antibiotic, fractionated by column chromatography and tested on clinical isolates – Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Candida albicans and Pseudomonas aeruginosa. The result of zones of inhibition was 45 mm, 47 mm, 48 mm, 49 mm and 47 mm in order of listing of organisms. Gas chromatography mass spectrometry analysis of the fractionated extract revealed the following compounds as being responsible for the observed inhibition – methylene chloride, heptanoic acid, octanoic acid, methyl hydrogen phthalate, 2,4-di-tert-butylphenol, dodecanoic acid, z-10-tetradecen-1-ol acetate, tetradecanoic acid, 2-tetradecyloxy-ethanol, n-hexadecanoic acid, hexadecanamide, octadecenamide and octadecanamide. A cocktail of organic compounds – fatty acids and amides that displayed strong antimicrobial ability, if well tapped, hold a future in the development of new antibiotics.

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Section: Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibiotic Activity of a Newly Discovered Aspergillus Species Isolated from Sewage Dump Site

M. O.,

C. O.,

C. U.

et al. 2024

African Journal of Biology and Medical Research

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“…Aspergillus, Fusarium, Penicillium, Verticillium, and Trichoderma are among the most common fungi genera in the soil [69,70]. Due to its fundamental function in suppressing pathogens, as well as endophytes, rhizospheric fungi and bacteria, these microorganisms have attracted the attention of researchers as a new source of valuable bioactive metabolites with antimicrobial activity [71][72][73]. Since antibiotic resistance is a serious global health concern [74], exploring the potential of these microorganisms to discover novel medicine is also of great urgency.…”

Section: Rhizospheric Microorganisms: An Overviewmentioning

confidence: 99%

Plant-Associated Microorganisms as a Potent Bio-Factory of Active Molecules against Multiresistant Pathogens

Cruz¹,

Bogas²,

Sousa³

2021

Antimicrobial Resistance - A One Health Perspective

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Antibiotic-resistant pathogens are a public health threat that has rapidly spread over decades due to continuous and uncontrolled administration of antimicrobial medicines, becoming an ever-increasing worldwide concern. Since the past decade, no significant innovations have been made, so the search for new compounds that face multidrug-resistant pathogens is critically important. Plant-symbiont microorganisms are capable of producing a variety of bioactive natural products, making it possible to treat several infectious diseases. Biotechnological processes using microorganisms have been increasing in recent years since the discovery of Paclitaxel, an important antimitotic produced by the endophyte Taxomyces andreanae. It was isolated for the first time from the native tree of Pacific Taxus brevifolia. Several studies have demonstrated the isolation and characterization of promising and potent substances capable of inhibiting these pathogens. In addition, both rhizospheric and endophytic communities represent an unexplored reserve of unique chemical structures for drug development. This chapter focuses on the potential of plant-derived microorganisms as a source of bioactive substances and the perspectives for further studies and their application.

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“…Antibiotics are widely distributed in the nature, where they play an important role in regulating the microbial population of soil, water, sewage and compost. Antimicrobial agents are natural products and produced by various types of bacteria and fungi [5].…”

Section: Introductionmentioning

confidence: 99%

“…Antibiotics in one form or the other has been in use for centuries [1]. Hundreds of these natural products have been identified and developed as therapeutic agents against many infectious diseases [5]. Routine screening is ongoing on bacteria for new bioactive compounds because they have provided many important bioactive compounds of high commercial value [6].…”

Section: Introductionmentioning

confidence: 99%

Screening for antibacterial producing bacteria isolated from soil in Keffi, Nasarawa, State, Nigeria

Makut¹,

Agu²,

Okey-Ndeche³

et al. 2023

GSC Biol. Pharm. Sci.

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Antibiotics are secondary metabolites that inhibit other competing cells to give competitive advantage to the microorganisms that produce them. This study is aimed at screening for antibacterial producing bacteria isolated from soil in Keffi. Standard microbiological methods were used in isolation and screening for antimicrobial of activity of crude extracts. Molecular identification of bacteria isolated were carried out using 16srRNA method. The antibacterial activity was carried out using agar well diffusion methods. The highest bacteria were Isolated from maize rhizosphere (90.0%) and the least was from groundnut rhizosphere (50.0%). Bacterial isolated and identified using 16srRNA were Pseudomonas fluorescens, Pseudomonas antarctica, Bacillus megaterium and Acetobacter aceti. The screening for antimicrobial producing bacteria showed that bacterial with laboratory code Gn3 (Pseudomonas fluorescens) showed activity against E. coli (8.57±1.01 mm), S. typhi (5.10±0.11 mm) and S. aureus (6.12±0.32 mm). Ma1 had activity against E. coli (8.57±1.01 mm), S. typhi (5.10±0.11 mm) and S. aureus (6.12±0.32 mm). Ma2 had activity against E. coli (7.56±1.33 mm) and S. typhi (4.54±0.34 mm) and Rc2 had activity only against S. aureus (12.12±0.98 mm) The Antibacterial activity of bacteria isolated and grown in complex medium with nitrogen source revealed that Pseudomonas fluorescens showed activity against the test organism E. coli (14.03±1.06mm), S. typhi (9.11±1.11mm) and S. aureus (5.12±1.02 mm). It was observed in this study that bacterial isolated from soil in Keffi were able to produce substance that that inhibit other bacterial.

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Isolation and screening of antibiotic producing actinomycetes from rhizosphere and agricultural soils

Cited by 12 publications

References 26 publications

Antibiotic Activity of a Newly Discovered Aspergillus Species Isolated from Sewage Dump Site

Antibiotic Activity of a Newly Discovered Aspergillus Species Isolated from Sewage Dump Site

Plant-Associated Microorganisms as a Potent Bio-Factory of Active Molecules against Multiresistant Pathogens

Screening for antibacterial producing bacteria isolated from soil in Keffi, Nasarawa, State, Nigeria

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