Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation

Zhang, Yingying; Luo, Xi; Li, Yin; Yin, Fengwei; Zheng, Wei‐Long; Fu, Yongqian

doi:10.3389/fbioe.2023.1183333

Cited by 3 publications

(2 citation statements)

References 31 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Deacetylation of chitin is done through the enzyme chitin deacetylase and deacetylation of chitin is done by alkaline solutions (such as NaOH) ( Zhu et al, 2019 ). The molecular weight of chitosan varies between 300 and 1,000 kDa, depending on the production method and the degree of deacetylation (between 60% and 95%), which makes it suitable for diverse applications ( Zhang et al, 2023 ).…”

Section: Biomaterials Used In Wound Healingmentioning

confidence: 99%

A review of the current state of natural biomaterials in wound healing applications

Ansari,

Darvishi

2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Skin, the largest biological organ, consists of three main parts: the epidermis, dermis, and subcutaneous tissue. Wounds are abnormal wounds in various forms, such as lacerations, burns, chronic wounds, diabetic wounds, acute wounds, and fractures. The wound healing process is dynamic, complex, and lengthy in four stages involving cells, macrophages, and growth factors. Wound dressing refers to a substance that covers the surface of a wound to prevent infection and secondary damage. Biomaterials applied in wound management have advanced significantly. Natural biomaterials are increasingly used due to their advantages including biomimicry of ECM, convenient accessibility, and involvement in native wound healing. However, there are still limitations such as low mechanical properties and expensive extraction methods. Therefore, their combination with synthetic biomaterials and/or adding bioactive agents has become an option for researchers in this field. In the present study, the stages of natural wound healing and the effect of biomaterials on its direction, type, and level will be investigated. Then, different types of polysaccharides and proteins were selected as desirable natural biomaterials, polymers as synthetic biomaterials with variable and suitable properties, and bioactive agents as effective additives. In the following, the structure of selected biomaterials, their extraction and production methods, their participation in wound healing, and quality control techniques of biomaterials-based wound dressings will be discussed.

show abstract

Section: Biomaterials Used In Wound Healingmentioning

confidence: 99%

A review of the current state of natural biomaterials in wound healing applications

Ansari,

Darvishi

2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Chitin is the second-largest natural polysaccharide after cellulose, and its exploitation and utilization is limited because it is insoluble in common solvents [1, 2]. However, traditional physical and chemical treatment methods can no longer meet the increasing demands for environmental protection and benefits, due to their drawbacks such as high energy consumption, environmental pollution, extensive use of strong acids and bases, and difficulty in controlling the structure of products [3–6]. As a result, greener and more efficient biodegradation has emerged as a new hotspot in the development of chitin resources [7, 8].…”

Section: Introductionmentioning

confidence: 99%

Streptomyces fuscus sp. nov., a brown-black pigment producing actinomycete isolated from dry mudflat sand

Xie,

Zhang,

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A novel Gram-stain-positive, aerobic actinobacterial strain, designated GXMU-J15T, was isolated from dry mudflat sand. A polyphasic approach was employed for its taxonomic characterization. The strain developed extensively branched yellowish white to light yellow substrate mycelia and white aerial mycelia, and produced smooth cylindrical spores in a loose straight spore chain on International Streptomyces Project 2–7 agar media. Strain GXMU-J15T grew at 20–50 °C (optimum, 35 °C), at pH 5.0–8.0 (optimum, pH 7.0) and in the presence of 0–8 % (w/v) NaCl. Analysis of 16S rRNA gene sequences indicated that strain GXMU-J15T represents a member of the genus Streptomyces . Strain GXMU-J15T showed the highest 16S rRNA gene sequence similarity to Streptomyces lusitanus CGMCC 4.1745T (99.1 %) and Streptomyces thermocarboxydus CGMCC 4.1883T (98.8 %). Phylogenetic tree analysis based on multilocus sequence analysis (MLSA) and whole genome sequence construction revealed that strain GXMU-J15T was most closely related to Streptomyces cupreus PSKA01T, Streptomyces cinnabarinus DSM 40467T and Streptomyces davaonensis JCM 4913T. The MLSA and genome-to-genome distances between strain GXMU-J15T and its relatives were 0.0418, 0.0443 and 0.0485 and 0.1237, 0.1188 and 0.1179, respectively. The results of orthologous average nucleotide identity and digital DNA–DNA hybridization analysis corroborated the results of the MLSA and whole genome sequence evolution analysis, indicating that the novel isolate represents a distinct species of the genus Streptomyces . The whole-cell sugars of strain GXMU-J15T were xylose, glucose and galactose. The characteristic diamino acid in the cell-wall hydrolysate was ll-diaminopimelic acid. The lipids contained diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylglycerides, phosphatidylcholine, two phospholipids of an unknown structure containing glucosamine, one unknown phospholipid and two unknown lipids. The major cellular fatty acid components were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The main respiratory quinone types were MK-9(H6) and MK-9(H8). The whole genome size of strain GXMU-J15T was 8.68 Mbp, with 71.23 mol% G+C content. Genomic analysis indicated that strain GXMU-J15T has the potential to synthesize polyketides, terpenes and a series of important antibiotics besides the gene cluster for melanin synthesis. Based on these genotypic and phenotypic data, strain GXMU-J15T is proposed to represent a new species of the genus Streptomyces named Streptomyces fuscus sp. nov. The type strain is GXMU-J15T (=MCCC 1K08211T=JCM 35917T).

show abstract

A new strain of Rhodococcus indonesiensis T22.7.1T and its functional potential for deacetylation of chitin and chitooligsaccharides

Xie,

Yin,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionChitin, abundant in marine environments, presents significant challenges in terms of transformation and utilization. A strain, T22.7.1T, with notable chitin deacetylation capabilities, was isolated from the rhizosphere of Acanthus ebracteatus in the North Sea of China. Comparative 16S rDNA sequence analysis showed that the new isolate had the highest sequence similarity (99.79%) with Rhodococcus indonesiensis CSLK01-03T, followed by R. ruber DSM 43338T, R. electrodiphilus JC435T, and R. aetherivorans 10bc312T (98.97%, 98.81%, and 98.83%, respectively). Subsequent genome sequencing and phylogenetic analysis confirmed that strain T22.7.1T belongs to the R. indonesiensis species. However, additional taxonomic characterization identified strain T22.7.1T as a novel type strain of R. indonesiensis distinct from CSLK01-03T.MethodsThis study refines the taxonomic description of R. indonesiensis and investigates its application in converting chitin into chitosan. The chitin deacetylase (RiCDA) activity of strain T22.7.1T was optimized, and the enzyme was isolated and purified from the fermentation products.ResultsThrough optimization, the RiCDA activity of strain T22.7.1T reached 287.02 U/mL, which is 34.88 times greater than the original enzyme’s activity (8.0 U/mL). The natural CDA enzyme was purified with a purification factor of 31.83, and the specific activity of the enzyme solution reached 1200.33 U/mg. RiCDA exhibited good pH and temperature adaptability and stability, along with a wide range of substrate adaptabilities, effectively deacetylating chitin, chitooligosaccharides, N-acetylglucosamine, and other substrates.DiscussionProduct analysis revealed that RiCDA treatment increased the deacetylation degree (DD) of natural chitin to 83%, surpassing that of commercial chitosan. Therefore, RiCDA demonstrates significant potential as an efficient deacetylation tool for natural chitin and chitooligosaccharides, highlighting its applicability in the biorefining of natural polysaccharides.

show abstract

Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation

Cited by 3 publications

References 31 publications

A review of the current state of natural biomaterials in wound healing applications

A review of the current state of natural biomaterials in wound healing applications

Streptomyces fuscus sp. nov., a brown-black pigment producing actinomycete isolated from dry mudflat sand

A new strain of Rhodococcus indonesiensis T22.7.1T and its functional potential for deacetylation of chitin and chitooligsaccharides

Contact Info

Product

Resources

About