Isolation and Purification of Various Mammalian Cells: Single Cell Isolation

Mukherjee, Srirupa; Malik, Parth; Mukherjee, Tapan Kumar

doi:10.1007/978-981-19-1731-8_7-1

Cited by 1 publication

(2 citation statements)

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“…These techniques are today those with the highest levels of specificity, purity and performance. 9 However, the high investment and operating costs, as a consequence of the equipment and reagents they require, have limited their use at a laboratory scale. In addition, the use of antibodies can cause changes in the structure of the cell membrane, and subsequently an additional step of detachment and recovery of the used antibodies is required.…”

Section: Extraction Methodsmentioning

confidence: 99%

“…However, these methods have multiple inefficiencies and are time‐consuming, leading to the development of immunochemical sorting technologies, including fluorescent antibody separation (FACS) and the use of magnetic beads (MACS). These techniques are today those with the highest levels of specificity, purity and performance 9 . However, the high investment and operating costs, as a consequence of the equipment and reagents they require, have limited their use at a laboratory scale.…”

Section: Introductionmentioning

confidence: 99%

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Aqueous two‐phase systems as a novel alternative in the primary recovery of human mesenchymal cells obtained from adipose tissue

Freire‐Flores,

Llanovarced‐Kawles,

Andrews

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUND: In recent years, therapies based on regenerative medicine have gained wide interest due to their potential in organ transplantation and in the treatment of multiple diseases. These cell‐based therapies rely mainly on the therapeutic potential of stem cells due to their self‐renewal, differentiation, capacities and immunosuppressive properties. Adipose tissue contains a large number of mesenchymal stem cells compared to other tissues and is easily accessible through non‐invasive techniques; hence the primary recovery of adipose‐derived mesenchymal stem cells (ASC) becomes a critical step. Aqueous two‐phase systems (ATPS) have shown promise as a separation technique for mesenchymal stem cells (MSC) due to their scalability, cost‐effectiveness, and compatibility. RESULTS: In this study, we investigated the partitioning behavior of the stromal vascular fraction cells, including ASC, in different ATPS compositions (polyethylene glycol, dextran, ficoll and Ucon). Among the three ATPS tested, most of the ASC consistently migrate to the top phase as indicated by immunostaining studies for CD73, CD90 and CD105 stem cell markers. Nevertheless, the Ficoll‐DEX system retrieves the greatest quantity of ASC recovered in the top phase (89% of total ASC), while the Ucon‐DEX system effectively eliminates the most contaminating cells (38%). CONCLUSIONS: Our findings provide insights into the conditions under which ASC and contaminant cells concentrate in opposite phases in different ATPS, which can contribute to the development of efficient and scalable separation systems for MSC in regenerative medicine and tissue engineering applications.This article is protected by copyright. All rights reserved.

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Section: Extraction Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%