Isolation and Purification of Kaempferol-3,7-O-α-L-Dirhamnopyranoside from<i>Siraitia grosvenori</i>Leaves by High-Speed Counter-Current Chromatograph and Its Free Radical Scavenging Activity

Li, Ying; Wei, Liuxin; Zhu, Zhiren; Pan, Yingming; Wang, Hengshan; Liu, Peizhen

doi:10.1080/01496395.2011.556101

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…HSCCC is used to isolate small numbers of compounds from plant matrices. However, the amount of ground plant powder needed is high, and can vary from 5 g (Liang et al 2011) up to 2 kg (Zhang et al 2011). The resulting eluents (large volumes of 90-95% ethanol or methanol) are evaporated and extracts are diluted in water.…”

Section: Purification Of Flavonoidsmentioning

confidence: 99%

Assessing the response of plant flavonoids to UV radiation: an overview of appropriate techniques

et al. 2014

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Flavonoids are a large group of plant secondary metabolites that are present in most plants, and are vital for plant growth, development and protection. Among the many functions of these compounds is their contribution to stress amelioration. The accurate identification and quantification of total or individual flavonoids in plants exposed to stressful conditions (e.g. ultraviolet radiation) is challenging due to their structural diversity. The present review provides the up to date knowledge and highlights trends in plant flavonoid analysis. The review covers all steps from the field to the laboratory, focussing on UV-B effects on flavonoids, and identifying critical issues concerning sample collection, pre-treatment, and extraction techniques and quantitative or qualitative analysis. A well-planned sampling and sample prehandling strategy is vital when capturing organ, tissue and developmentalstage dependent changes in flavonoids, as well as the dynamic changes due to time of UV-exposure and diurnal or seasonal parameters. A range of advanced extraction and purification techniques can facilitate the quantitative recovery transfer of flavonoids to solvents. The advantages and disadvantages of analytical methods, including chromogenic assays, liquid and thin-layer chromatography, mass spectrometry, NMR detection, and non-destructive in situ fluorescent analysis need to be consciously evaluated in the context of the specific biological question posed. Thus, no one method can be applied to every single study of flavonoid. The message of this review is that researchers will need to carefully consider the biological process that they intend to study, and select an analytical method that optimally matches their specific objectives.

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Section: Purification Of Flavonoidsmentioning

confidence: 99%

Assessing the response of plant flavonoids to UV radiation: an overview of appropriate techniques

et al. 2014

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“…They used 90 mg of the plant extract in a two-phase solvents system. Yang and his coauthors isolated kaempferol-3,7-O-a-L-dirhamnopyranoside from a plant extract and its structure was identified using 1 H-NMR, MS and 13 C-NMR [ 19 ].…”

Section: Isolation Of Kaempferol From Natural Sourcesmentioning

confidence: 99%

Kaempferol as a Dietary Anti-Inflammatory Agent: Current Therapeutic Standing

et al. 2020

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Inflammation is a physiological response to different pathological, cellular or vascular damages due to physical, chemical or mechanical trauma. It is characterized by pain, redness, heat and swelling. Current natural drugs are carefully chosen as a novel therapeutic strategy for the management of inflammatory diseases. Different phytochemical constituents are present in natural products. These phytochemicals have high efficacy both in vivo and in vitro. Among them, flavonoids occur in many foods, vegetables and herbal medicines and are considered as the most active constituent, having the ability to attenuate inflammation. Kaempferol is a polyphenol that is richly found in fruits, vegetables and herbal medicines. It is also found in plant-derived beverages. Kaempferol is used in the management of various ailments but there is no available review article that can summarize all the natural sources and biological activities specifically focusing on the anti-inflammatory effect of kaempferol. Therefore, this article is aimed at providing a brief updated review of the literature regarding the anti-inflammatory effect of kaempferol and its possible molecular mechanisms of action. Furthermore, the review provides the available updated literature regarding the natural sources, chemistry, biosynthesis, oral absorption, metabolism, bioavailability and therapeutic effect of kaempferol.

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“…The use of preparative-scale CCS is also important, as bioactive natural products may be present as minor metabolites in an organism’s metabolome. Table S10, Supporting Information, summarizes the use of CCS to prepare fractions for bioassay screening of natural products in articles published since 2007. ,,,,,,,,,,,– ,,,,,,,,, ,,– ,,,– …”

Section: Bioactivity and Nonplant Applicationsmentioning

confidence: 99%

“…When planned and optimized properly, the selectivity and separation potential of CCS may yield impressive results. This can be exemplified by the relatively large number of recent articles reporting on the purification of one natural product in a single CCS step (Table S12, Supporting Information). ,,,,,,,,,,,− While rigorous purity evaluations remain the exception in these studies, they still demonstrate the growing role of CCS in natural product research laboratories and how researchers have explored successfully the potential of CSS as a high-resolution preparative separation method.…”

Section: Conclusion and Outlookmentioning

confidence: 99%

Countercurrent Separation of Natural Products: An Update

et al. 2015

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This work assesses the current instrumentation, method development, and applications in countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC), collectively referred to as countercurrent separation (CCS). The article provides a critical review of the CCS literature from 2007 since our last review (J. Nat. Prod.2008, 71, 1489–1508), with a special emphasis on the applications of CCS in natural products research. The current state of CCS is reviewed in regard to three continuing topics (instrumentation, solvent system development, theory) and three new topics (optimization of parameters, workflow, bioactivity applications). The goals of this review are to deliver the necessary background with references for an up-to-date perspective of CCS, to point out its potential for the natural product scientist, and thereby to induce new applications in natural product chemistry, metabolome, and drug discovery research involving organisms from terrestrial and marine sources.

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Isolation and Purification of Kaempferol-3,7-O-α-L-Dirhamnopyranoside fromSiraitia grosvenoriLeaves by High-Speed Counter-Current Chromatograph and Its Free Radical Scavenging Activity

Cited by 9 publications

References 24 publications

Assessing the response of plant flavonoids to UV radiation: an overview of appropriate techniques

Assessing the response of plant flavonoids to UV radiation: an overview of appropriate techniques

Kaempferol as a Dietary Anti-Inflammatory Agent: Current Therapeutic Standing

Countercurrent Separation of Natural Products: An Update

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