Isolation and purification of intralamellar vesicles from <i>Lemna minor</i> L. chloroplasts

Picher, Maryse; Grenier, Gilles; Purcell, Marc; Proteau, L.; Beaumont, G.

doi:10.1111/j.1469-8137.1993.tb03774.x

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…Linolenic acid accounted for <3% of the total fatty acid complement in phloem, but constituted 20-40% of the total fatty acids in leaf microsomes and cytosol. Moreover, linolenic acid typically contributes up to 70% of galactolipid fatty acids in plastoglobuli (Picher et al 1993). Similarly, storage triacylglycerols of oil bodies contain high levels of unsaturated fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of lipid in phloem sap of canola

Madey

Nowack

Thompson

2002

Planta

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Phloem isolated from canola (Brassica napus L.) stems was found to contain phospholipid, diacylglycerol, triacylglycerol, steryl and wax esters, and comparatively high concentrations of unesterified fatty acids. Indeed, the composition of phloem lipid was markedly different from that of microsomal membranes and cytosol isolated from both leaves and stems. Specifically, phloem lipid consisted predominantly of unesterified fatty acids and was enriched in medium-chain fatty acids, in particular, lauric, myristic and pentadecanoic acids. This unique composition also distinguished phloem lipid from that of well-characterized cytosolic lipid particles such as oil bodies found in plant cells. Moreover, levels of medium-chain fatty acids in the phloem increased when canola plants were stressed by exposure to sublethal doses of ultraviolet irradiation. Phloem levels of lauric acid, for example, increased by 11-fold upon treatment with sublethal ultraviolet irradiation. Spherical lipid particles were discernible in isolated phloem sap by electron microscopy, suggesting that the lipid in phloem is in the form of lipid particles. The presence of lipid in phloem may be reflective of long-distance lipid transport in plants, primarily in the form of free fatty acids.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of lipid in phloem sap of canola

Madey

Nowack

Thompson

2002

Planta

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“…Moreover, Satoh et al (1998) have shown that in vitro the protein is able to remove Chl from thylakoids and, hence, to dismantle pigment-protein complexes. A contrasting possibility of Chl transfer from thylakoids across the stroma to the envelope is suggested by the presence of Chl in the plastoglobules (Young et al, 1991;Picher et al, 1993). Plastoglobules contain a specific set of proteins (Kessler et al, 1999) and should not be underestimated as products of passive accumulation of lipophilic material.…”

Section: Detoxification Of Chlorophyllmentioning

confidence: 96%

Senescence and Cell Death in Plant Development: Chloroplast Senescence and its Regulation

Matile

2001

Regulation of Photosynthesis

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“…The plastoglobules of wild-type chloroplasts had a translucent appearance, in contrast to those of the mutant chloroplasts, which had strongly osmiophilic structures. In addition to increases in their number and size, a loss of the osmiophilic character of plastoglobules has been shown to be associated with chloroplast senescence, reflecting an alteration in the lipid composition of the plastoglobules (Picher et al, 1993;Špundova et al, 2003). This alteration probably reflects the exchange of lipid molecules between thylakoids and plastoglobules, including deposition of compounds released during thylakoid breakdown (Austin et al, 2006).…”

Section: Researchmentioning

confidence: 99%

The thylakoid protease Deg2 is involved in stress‐related degradation of the photosystem II light‐harvesting protein Lhcb6 in Arabidopsis thaliana

et al. 2011

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Summary• The thylakoid protease Deg2 is a serine-type protease peripherally attached to the stromal side of the thylakoid membrane. Given the lack of knowledge concerning its function, two T-DNA insertion lines devoid of Deg2 were prepared to study the functional importance of this protease in Arabidopsis thaliana.• The phenotypic appearance of deg2 mutants was studied using a combination of stereo and transmission electron microscopy, and short-stress-mediated degradation of apoproteins of minor light-harvesting antennae of photosystem II (PSII) was analysed by immunoblotting in the mutants in comparison with wild-type plants.• Deg2 repression produced a phenotype in which reduced leaf area and modified chloroplast ultrastructure of older leaves were the most prominent features. In contrast to the wild type, the chloroplasts of second-whorl leaves of 4-wk-old deg2 mutants did not display features typical of the early senescence phase, such as undulation of the chloroplast envelope and thylakoids. The ability to degrade the photosystem II light-harvesting protein Lhcb6 apoprotein in response to brief high-salt, wounding, high-temperature and high-irradiance stress was demonstrated to be impaired in deg2 mutants.• Our results suggest that Deg2 is required for normal plant development, including the chloroplast life cycle, and has an important function in the degradation of Lhcb6 in response to short-duration stresses.

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Isolation and purification of intralamellar vesicles from Lemna minor L. chloroplasts

Cited by 8 publications

References 30 publications

Isolation and characterization of lipid in phloem sap of canola

Isolation and characterization of lipid in phloem sap of canola

Senescence and Cell Death in Plant Development: Chloroplast Senescence and its Regulation

The thylakoid protease Deg2 is involved in stress‐related degradation of the photosystem II light‐harvesting protein Lhcb6 in Arabidopsis thaliana

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