Isolation and purification of glutathione peroxidase

Shulgin, K. K.; Попова, Т. Н.; Rakhmanova, T. I.

doi:10.1134/s0003683808030034

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…It has been shown that catalase from camel liver, SOD from sea cucumber, and GSHPx from rat liver had the optimal temperature of 45°C, 40°C, and 32°C, respectively ( Shul'Gim et al., 2008 , Chafik et al., 2017 , Li et al., 2018 ). GSHPx was found to be stable after heating to 63°C and lose its activity when heated to 80°C–90°C, catalase had a similar sensitivity, whereas SOD had a somewhat higher resistance to heat ( Mei et al., 1994 , Lindmark-Månsson et al., 2001 ).…”

Section: Resultsmentioning

confidence: 99%

Lipid oxidation induced by heating in chicken meat and the relationship with oxidants and antioxidant enzymes activities

et al. 2020

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The aim of the current research was to examine lipid oxidation in chicken meat heated to different temperatures followed by refrigerator storage and the factors contributing to lipid oxidation. It showed that lipid oxidation was significantly promoted when meat was heated up to 70°C and stored for 2 and 4 D as measured by thiobarbituric acid reactive substance. The monounsaturated fatty acids and polyunsaturated fatty acids also decreased significantly ( P < 0.05) with the increase of heating temperature. The liberation of nonheme iron and increase of hydroxyl radical were observed in heated chicken meat, and the activities of antioxidant enzymes was decreased considerably at higher temperatures. The changes of these prooxidants and antioxidants might constitute a possible mechanism for the stronger lipid oxidation in heated meat.

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Section: Resultsmentioning

confidence: 99%

Lipid oxidation induced by heating in chicken meat and the relationship with oxidants and antioxidant enzymes activities

et al. 2020

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“…Thus a compartmentalization of its conversion might be beneficial limiting its indirect strong oxidizing capacity. Actually, the nature, the site and the moment of production of protective ROS are Importantly, the optimal pH for SOD, GPX and CAT activity is reported to be around 7.8, 7.4 and 7.0, respectively [25]; see also Sigma-Aldrich Product Information and references therein (www.sigma-aldrich.com). The optimal pH may explain why only CAT increases its activity when acidosis (pH lower than 7.4) persists in early reperfusion [1,2,4].…”

Section: Discussionmentioning

confidence: 99%

Acidic infusion in early reperfusion affects the activity of antioxidant enzymes in postischemic isolated rat heart

Penna

Perrelli

Tullio

et al. 2013

Journal of Surgical Research

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Distribution of selected essential (Co, Cu, Fe, Mn, Mo, Se, and Zn) and nonessential (Cd, Pb) trace elements among protein fractions from hepatic cytosol of European chub (Squalius cephalus L.)

Krasnići

Dragun

Erk

et al. 2012

Environ Sci Pollut Res

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Association of selected essential (Co, Cu, Fe, Mn, Mo, Se, and Zn) and nonessential (Cd, Pb) trace elements with cytosolic proteins of different molecular masses was described for the liver of European chub (Squalius cephalus) from weakly contaminated Sutla River in Croatia. The principal aim was to establish basic trace element distributions among protein fractions characteristic for the fish living in the conditions of low metal exposure in the water. The fractionation of chub hepatic cytosols was carried out by size exclusion high performance liquid chromatography (SE-HPLC; Superdex™ 200 10/300 GL column), and measurements were performed by high resolution inductively coupled plasma mass spectrometry (HR ICP-MS). Elution profiles of essential elements were mostly characterized by broad peaks covering wide range of molecular masses, as a sign of incorporation of essential elements in various proteins within hepatic cytosol. Exceptions were Cu and Fe, with elution profiles characterized by sharp, narrow peaks indicating their probable association with specific proteins, metallothionein (MT), and ferritin, respectively. The main feature of the elution profile of nonessential metal Cd was also single sharp, narrow peak, coinciding with MT elution time, and indicating almost complete Cd detoxification by MT under the conditions of weak metal exposure in the water (dissolved Cd concentration ≤0.3 μg L(-1)). Contrary, nonessential metal Pb was observed to bind to wide spectrum of proteins, mostly of medium molecular masses (30-100 kDa), after exposure to dissolved Pb concentration of ~1 μg L(-1). The obtained information within this study presents the starting point for identification and characterization of specific metal/metalloid-binding proteins in chub hepatic cytosol, which could be further used as markers of metal/metalloid exposure or effect on fish.

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Isolation and purification of glutathione peroxidase

Cited by 5 publications

References 6 publications

Lipid oxidation induced by heating in chicken meat and the relationship with oxidants and antioxidant enzymes activities

Lipid oxidation induced by heating in chicken meat and the relationship with oxidants and antioxidant enzymes activities

Acidic infusion in early reperfusion affects the activity of antioxidant enzymes in postischemic isolated rat heart

Distribution of selected essential (Co, Cu, Fe, Mn, Mo, Se, and Zn) and nonessential (Cd, Pb) trace elements among protein fractions from hepatic cytosol of European chub (Squalius cephalus L.)

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