Isolation and Purification of Fatty Acid Steryl Esters from Soybean Oil Deodorizer Distillate

Abstract

Depending on the source, deodorizer distillates usually have significantly different characteristics, uses, and value. Soybean oil deodorizer distillate (SODD) has been suggested as an alternative to marine animals as a natural source of squalene and a good raw material for the production of fatty acid steryl esters (FASEs), tocopherols, free phytosterols and fatty acids. The purpose of this work was to isolate and purify natural FASEs from SODD by a suitable method without degradation of the FASEs. Modified Soxhlet extraction, modified silica gel column chromatography, and binary solvent extraction were employed sequentially in this study to obtain FASEs from SODD. FASEs with a purity of 86.74% and a total recovery of 85.32% could be obtained in the final product.

Irresolvable complex mixture of hydrocarbons in soybean oil deodorizer distillate

“…2, and hence it can be concluded that no unsaturated bonds exist in the unknown ICM. 13 C-NMR result in Fig. 2 shows that several peaks appear at 10-40 ppm chemical shifts and thus confirms that C atoms in the unknowns are only attached to either C or H atoms.…”

“…Soybean oil deodorizer distillate (SODD) is a byproduct of the soybean oil refining process. Gunawan et al [12,13] not only succeeded in isolating squalene and fatty acid steryl esters from SODD, but also found that ca. 44 wt% of the non-polar lipid fraction (NPLF) of SODD are unidentified/unknown HCs.…”

Recovery of Phytosterols from Waste Residue of Soybean Oil Deodorizer Distillate

“…Spots for FASEs and steroidal hydrocarbons were detected by spraying with a fresh solution of ferric chloride (50mg) in a mixture of water (90ml), acetic acid (5ml) and sulfuric acid (5ml). After the plates were heated at 100°C for 3-5 min, FASEs and steroidal hydrocarbons were detected by a red-violet color (Gunawan, et al, 2008).…”

“…Guclu-Ustundag and Temelli (2007) developed a semi-continuous column fractionation of canola oil deodorizer distillate using supercritical CO 2 (SCCO 2 ) to determine the feasibility of value-added processing of this feed material for the recovery of bioactive components such as sterols and tocopherols and the effect of operating conditions on extract yield and separation efficiency. Gunawan et al (2008) described a suitable method (without degradation of the FASEs) i.e. modified Soxhlet extraction, modified silica gel column chromatography and binary solvent extraction to isolate and purify natural FASEs from SODD.…”

Production of Steryl Esters from Vegetable Oil Deodorizer Distillates by Enzymatic Esterification

“…[2][3][4] Phytosterols have been found to be effective in lowering plasma cholesterol concentrations and prevention of cardiovascular diseases, 5,6 while tocopherols, which are physiologically active as vitamin E, are natural antioxidants that have an important role in the protection of fats and oils from oxidation. 7,8 Despite previous efforts, [9][10][11][12][13][14][15][16][17][18] the valorization of deodorizer distillates still remains a challenge since the recovery and purification yields of the target compounds are not satisfactory. A high content of pesticides has been found in deodorizer distillates, 19 making their valorization dependent on the removal of pesticides, due to stringent legislation in the majority of countries concerning the pesticide content in food additives.…”