Isolation and purification of a tissue plasminogen activator and its comparison with urokinase

Plasminogen activator activity in cultures from human tissues. An immunological and histochemical study

“…In vivo, UK is found in urine while tissues and other body fluids yield activators that differ from UK immunologically (8) and in other properties (3,12). Although precise origin and interrelationship of the various naturally occurring activators in man, particularly tissue, plasma, and urine activators have not been entirely clarified, it has been shown that UK is produced by kidney cells (8,9).…”

“…Ac-tivator in urine, urokinase (UK), is produced by kidney cells (9) but may also be derived from other sources (10,11). Urokinase, tissue, and plasma activators differ from each other immunologically (8) and in other properties (3,12). However, exact relationship among these activators, their origin, and their role in blood and local fibrinolytic events are not clear.…”

Thrombolysis with human extrinsic (tissue-type) plasminogen activator in dogs with femoral vein thrombosis.

“…"Extrinsic" or "tissue-type" plasminogen activators extracted from blood (8,9), vessel perfusates (10), or from tissues (11)(12)(13) have the property to preferentially activate plasminogen in the presence of fibrin (14)(15)(16), thereby confining the activation process to the immediate environment of a formed thrombus.…”

Purification of a Plasminogen Activator Inhibitor Indistinguishable from α₁-Antitrypsin and an Urokinase Inhibitor in Pregnancy Plasma

“…M-Partigen® a r antitrypsin immunodiffusion plate and protein Standard plasma, lyophilized (Behringwerke). Preparations of bovine, plasmin ogen-rich fibrinogen, bovine thrombin (Leo Pharmaceuticals, Copenhagen, Denmark), human urokinase (Leo Pharmaceuticals) and tissue plasminogen activator from pregnant hog ovaries [24] were the same as those previously reported [34]. Plasmin: glycerol-acti vated human plasmin, 25 CU per vial (Kabi, Stockholm, Sweden).…”