Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus

Nishida, Yukiko; Suzuki, Ken-ichi; Kumagai, Yuya; Tanaka, Hiroyuki; Inoue, Akira; Ojima, Takao

doi:10.1016/j.biochi.2007.03.015

Cited by 79 publications

(65 citation statements)

References 34 publications

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“…SnEG54 cDNAs without a stop codon were amplified from pCR2-SnEG54 cDNA [17] by PCR with KOD (Toyobo, Tokyo, Japan) using the following primers: 5'-GCCATGTTGCCTTTAATCTTG-3' (SnEG54-sense) and 5'-CATGTTGAGTTGCTTGAGGCC-3' (SnEG54-antisense). The amplified fragments were subcloned into pBluescript II SK+ (Stratagene, La Jolla, CA) at the EcoRV site (pBS-SnEG54).…”

Section: Methodsmentioning

confidence: 99%

“…Next, we examined cellulase activity of SnEG54 prepared from the cytosol and the culture medium as reported previously [17]. pcDNA3-MycHis-SnEG54 (20 μg) or Mock was transfected into HEK293T cells (3 x 10 6 cells/10 ml culture medium).…”

Section: Cellulase Activity Of Sneg54 Expressed In Mammalian Cellsmentioning

confidence: 99%

“…1,4--glucosidase (EC 3.2.1.21) is required for complete de-polymerization of cellulose to glucose. These enzymes have been isolated from bacteria and fungi [1], plants [2], molds [3], microbes from animal intestines [4], and herbivorous invertebrates such as arthropods [5][6][7][8][9][10], nematodes [10], mollusks [11][12][13][14][15][16] and an echinoderm [17].…”

Section: Introductionmentioning

confidence: 99%

“…The majority of animal cellulases belong to GHF9 and a cellulase from sea urchin, SnEG54, was also classified under GHF9 [17]. SnEG54 exhibited a high level of hydrolytic activity toward carboxymethyl cellulose (CMC) at 35°C and pH 6.5 and also degraded a cellulose Introduction of retroviral vectors containing the gene of interest into suitable packaging cells enables production of infectious retroviruses, and these particles can infect target cells and stably transmit the gene of interest into chromosomes.…”

Section: Introductionmentioning

confidence: 99%

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Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines

Okumura

Kameda

Ojima

et al. 2010

Biochemical and Biophysical Research Communications

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We previously identified the cellulase SnEG54 from Japanese purple sea urchin Strongylocentrotus nudus, the molecular mass of which is about 54 kDa on SDS-PAGE.It is difficult to express and purify a recombinant cellulase protein using bacteria such as E. coli or yeast. In this study, we generated mammalian expression vectors encoding SnEG54 to transiently express SnEG54 in mammalian cells. Both SnEG54 expressed in mammalian cells and SnEG54 released into the culture supernatant showed hydrolytic activity toward carboxymethyl cellulose. By using a retroviral expression system, we also established a mammalian cell line that constitutively produces SnEG54.Unexpectedly, SnEG54 released into the culture medium was not stable, and the peak time showing the highest concentration was approximately 1 to 2 days after seeding into fresh culture media. These findings suggest that non-mammalian sea urchin cellulase can be generated in human cell lines but that recombinant SnEG54 is unstable in culture medium due to an unidentified mechanism.3

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Section: Methodsmentioning

confidence: 99%

Section: Cellulase Activity Of Sneg54 Expressed In Mammalian Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines

Okumura

Kameda

Ojima

et al. 2010

Biochemical and Biophysical Research Communications

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“…Besides, a-amylase and cellulase were purified and characterized from the digestive organ of Strongylocentrotus nudus (Nakatani and Kobayashi, 1996;Nishida et al, 2007). More recently, we purified subtilase from the digestive organ of the short-spined sea urchin (Strongylocentrotus intermedius) by a combination of ion-exchange chromatography and gel filtration.…”

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confidence: 99%

Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius

Ura

Hasegawa

Tanaka

et al. 2011

The Anatomical Record

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Subtilase, a major protease in the short-spined sea urchin (Strongylocentrotus intermedius), was isolated and used as antigen for the subsequent production of a specific polyclonal antibody. Immunoreactive cells were observed by immunohistochemical analysis in granules in the anterior and posterior stomach and the anterior intestine. These granules, which were most numerous in the anterior stomach, also stained intensely with methylene blue-Azure II. However, granules in cells of the esophagus, posterior intestine, and rectum were not stained by this antibody. We conclude that subtilase mainly localizes in the stomach and anterior intestine of the sea urchin. Anat Rec, 295:73-77, 2012. V V C 2011 Wiley Periodicals, Inc.

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Waste paper to bioethanol: Current and future prospective

Al-Azkawi

Elliston²,

Al-Bahry

et al. 2019

Biofuels Bioprod Bioref

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Bioethanol is considered to be a possible substitute for non‐renewable fossil fuels. Biofuels, including bioethanol, are gradually replacing coal and oil for energy production. It is expected that, by 2040, fossil fuels’ contribution to the world's energy demands will only be 25% with the remaining contribution made up by biofuels. One of the major factors affecting ethanol production is the cost of raw materials, which can be substantially reduced by utilizing industrial or agricultural lignocellulosic wastes. Using waste paper as a feedstock for bioethanol production would reduce the production cost and may eliminate problems in managing this waste material in the environment. The cellulose, hemicellulose, and lignin in waste paper are less complicated and so only mild pretreatment is needed before enzymatic hydrolysis and fermentation. This review covers topics such as bioethanol as a promising alternative to petroleum fuels, feedstocks for bioethanol production, lignocellulosic technology and challenges for ethanol production, advantages and disadvantages of pretreatments methods, waste paper as feedstock for ethanol production, processing of waste paper to ethanol, and the different integration methods of hydrolysis and fermentation. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.

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Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus

Cited by 79 publications

References 34 publications

Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines

Expression of recombinant sea urchin cellulase SnEG54 using mammalian cell lines

Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius

Waste paper to bioethanol: Current and future prospective

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