Isolation and preliminary screening of potentially probiotic Weissella confusa strains from healthy human feces by culturomics

Wen-qian, Wang; Liu, Wugao; Chu, Weihua

doi:10.1016/j.micpath.2020.104356

Cited by 14 publications

(15 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H 2 O 2 is the most influential metabolite produced by lactic acid bacteria to inhibit pathogens in the lower female genital tract (FGT) (Hutt et al, 2016). The lactobacilli strain was inoculated onto an agar plate containing tetramethylbenzidine (TMB) and lagan peroxidase incubated at 37°C for 48 h (Wang et al, 2020). The plate was placed in open air and the colonies of the peroxide-producing strain turned to a blue colour.…”

Section: Measurement Of Hydrogen Peroxide Productionmentioning

confidence: 99%

Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing

Jiang

Lessing

et al. 2022

Journal of Applied Microbiology

Self Cite

View full text Add to dashboard Cite

Aim: Lactobacillus species are the dominant microorganisms in the vaginal microbiota of healthy women and play an important role in the defence against pathogens.This study aimed to evaluate probiotic potential of Lactiplantibacillus plantarum strain P1 isolated from healthy woman's vaginal discharge for its further utilization as a promising candidate strain in the treatment of bacterial vaginosis caused by Gardnerella vaginalis.Methods and Results: Ten lactobacilli strains from a woman's vaginal discharge were evaluated for their probiotic potential, including growth capacity at different pH levels (pH 3.5-4.5), acid production, hydrogen peroxide production capacity, antibacterial activity and susceptibility to antibiotics. Moreover, in vitro safety assay haemolytic activity and mutagenicity were investigated for safety assessment. In vivo Caenorhabditis elegans infection model was used to investigate the anti-infection effect of selected isolates. We found that lactobacilli strain P1 showed strong growth ability in low acid environment, produced acid, hydrogen peroxide, had the strongest antibacterial activity against G. vaginalis and was highly susceptible to the tested antibiotics. When assayed for the safety, strain P1 showed no haemolytic activity and had no effect of mutagenicity. Moreover, P1 significantly increased the lifespan of C. elegans against G. vaginalis infection. Combined with the results of 16S rRNA gene sequencing, morphological and physiological characteristic, the strain was identified as Lactiplantibacillus plantarum. Conclusion:Lactiplantibacillus plantarum strain P1 proves to be a promising candidate strain in the treatment of bacterial vaginosis caused by G. vaginalis.Significance and Impact of the Study: Conventional antibiotic therapy for bacterial vaginosis has led to the accelerated process of bacterial drug resistance. Probiotics are potentially an alternative method for bacterial vaginosis therapy. This finding provides bacterial resources for keeping pathogens away from the vagina. We believe L. plantarum P1 may be used as vaginal probiotics and be useful to prevent or treat bacterial vaginitis.

show abstract

Section: Measurement Of Hydrogen Peroxide Productionmentioning

confidence: 99%

Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing

Jiang

Lessing

et al. 2022

Journal of Applied Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Their presence in food matrices and many spontaneous fermentation processes of vegetables, dairy, and cereals (Wang et al, 2011 ; Zannini et al, 2013 , 2018 ; Mun and Chang, 2020 ) reveals their role in these fermentative processes, in which they exert an influence on the quality, and especially on the texture of foods. In addition, they have been isolated from the gut, oral cavity, breast milk (Martín et al, 2007 ; Albesharat et al, 2011 ), and urogenital and gastrointestinal tracts of humans (Lee et al, 2012 ), as well as from many animals, such as giant pandas and rainbow trout, and from the skin, milk, and gastrointestinal tracts of vertebrates (Xiong et al, 2019 ; Mortezaei et al, 2020 ; Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species

Fanelli

Montemurro²,

Chieffi³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

In this study, the genomes of the Weissella (W.) beninensis, W. diestrammenae, W. fabalis, W. fabaria, W. ghanensis, and W. uvarum type strains were sequenced and analyzed. Moreover, the ability of these strains to metabolize 95 carbohydrates was investigated, and the genetic determinants of such capability were searched within the sequenced genomes. 16S rRNA gene and genome-based-phylogeny of all the Weissella species described to date allowed a reassessment of the Weissella genus species groups. As a result, six distinct species groups within the genus, namely, W. beninensis, W. kandleri, W. confusa, W. halotolerans, W. oryzae, and W. paramesenteroides species groups, could be described. Phenotypic analyses provided further knowledge about the ability of the W. beninensis, W. ghanensis, W. fabaria, W. fabalis, W. uvarum, and W. diestrammenae type strains to metabolize certain carbohydrates and confirmed the interspecific diversity of the analyzed strains. Moreover, in many cases, the carbohydrate metabolism pathway and phylogenomic species group clustering overlapped. The novel insights provided in our study significantly improved the knowledge about the Weissella genus and allowed us to identify features that define the role of the analyzed type strains in fermentative processes and their biotechnological potential.

show abstract

“…They have been isolated from various fermented foods, such as yoghurt, sourdough, and kimchi ( Wang et al, 2011 ; Zannini et al, 2018 ; Mun and Chang, 2020 ; Valerio et al, 2020 ), and play important roles in the process of fermentation, thereby influencing the texture and taste of foods. They also exist in the guts of humans and vertebrate animals, such as giant pandas and rainbow trout ( Xiong et al, 2019 ; Mortezaei et al, 2020 ; Wang et al, 2020 ), their functions involve reducing depressive-like behavior ( Sandes et al, 2020 ), influencing gut permeability and intestinal epithelial regeneration ( Prado et al, 2020 ), killing harmful bacteria ( Dey and Kang, 2020 ; Lee et al, 2020 ; Pelyuntha et al, 2020 ), affecting host metabolism ( Elshaghabee et al, 2020 ), and preventing cancer cell proliferation ( Le et al, 2020 ). Some Weissella species may be beneficial to plants because they inhibit the infection of plant pathogenic fungi, such as Fusarium verticillioides ( Quattrini et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis

et al. 2021

View full text Add to dashboard Cite

The genus Weissella is attracting an increasing amount of attention because of its multiple functions and probiotic potential. In particular, the species Weissella confusa is known to have great potential in industrial applications and exhibits numerous biological functions. However, the knowledge on this bacterium in insects is not investigated. Here, we isolated and identified W. confusa as the dominant lactic acid bacteria in the gut of the migratory locust. We named this strain W. confusa LM1, which is the first genome of an insect-derived W. confusa strain with one complete chromosome and one complete plasmid. Among all W. confusa strains, W. confusa LM1 had the largest genome. Its genome was the closest to that of W. confusa 1001271B_151109_G12, a strain from human feces. Our results provided accurate evolutionary relationships of known Weissella species and W. confusa strains. Based on genomic analysis, the pan-genome of W. confusa is in an open state. Most strains of W. confusa had the unique genes, indicating that these strains can adapt to different ecological niches and organisms. However, the variation of strain-specific genes did represent significant correlations with their hosts and ecological niches. These strains were predicted to have low potential to produce secondary metabolites. Furthermore, no antibiotic resistance genes were identified. At the same time, virulence factors associated with toxin production and secretion system were not found, indicating that W. confusa strains were not sufficient to perform virulence. Our study facilitated the discovery of the functions of W. confusa LM1 in locust biology and their potential application to locust management.

show abstract

Isolation and preliminary screening of potentially probiotic Weissella confusa strains from healthy human feces by culturomics

Cited by 14 publications

References 24 publications

Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing

Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing

Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species

Complete Genome Sequence of Weissella confusa LM1 and Comparative Genomic Analysis

Contact Info

Product

Resources

About