Isolation and plaque purification of a syncytial forming component of myxoma virus

Bijlenga, G.; Duclos, Philippe

doi:10.1016/0147-9571(89)90053-2

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“…The other strain SA, which shows genetic relationship with the strain Lu, induces large syncytia on cell culture, which is suggestive according to some authors of lower pathogenicity of the strain (Bulenga and Duclos, 1989). This assumption was not confirmed in the challenge strain SA.…”

Section: Discussionmentioning

confidence: 99%

Atypical Myxomatosis – Virus Isolation, Experimental Infection of Rabbits and Restriction Endonuclease Analysis of the Isolate

Pšikal¹,

Smíd

Rodák

et al. 2003

Journal of Veterinary Medicine, Series B

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Atypical form of myxomatosis, which caused non-lethal and clinically mild disease in domestic rabbits 1 month after immunization with a commercially available vaccine MXT, is described. The isolated myxoma virus designated as Litovel 2 (Li-2) did not induce systemic disease following subcutaneous and intradermal applications in susceptible experimental rabbits but led to the immune response demonstrated by ELISA. No severe disease was induced in those Li-2 inoculated rabbits by challenge with the virulent strains Lausanne (Lu) or Sanar (SA), while the control animals showed nodular form of myxomatosis with lethal course of the illness. Restriction fragment length polymorphism (RFLP) of genomic DNA with KpnI and BamHI endonucleases was used for genetic characterization of the Li-2 isolate, the vaccine strain MXT and both virulent strains Lu and SA, respectively. In general, RFLP analysis has shown to be informative for inferring genetic relatedness between myxoma viruses. Based on restriction endonuclease DNA fragment size distribution, it was evident that the pathogenic strain SA is genetically related to the reference strain Lu and the isolate Li-2 is more related, but not identical, to the vaccination strain MXT.

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Section: Discussionmentioning

confidence: 99%