Isolation and phylogenetic analysis of Getah virus from a commercial modified live vaccine against porcine reproductive and respiratory syndrome virus

Zhou, Feng; Wang, Aojie; Chen, Lu; Wang, Xingang; Cui, Dandan; Chang, Hongtao

doi:10.1016/j.mcp.2020.101650

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…1I and 1J), and housed in a cage with their GETV-free littermates. Total RNA was extracted from tissue, including spleen, lung, cerebral cortex, and various lymph nodes using TRIzol reagent after 5 days post inoculation (DPI) (Kumanomido et al, 1988), and subjected to reverse transcriptionpolymerase chain reaction (RT-PCR) for GETV detection using specific primers (GETV-F: 5′-ACCGAAGAAGCCGAAGAA-3′, and GETV-R: 5′-GCACTCRAGGTCATACTTG-3′) (Zhou et al, 2020) (Supplemental Fig. 1G and 1H).…”

Section: Infectiousness Of Mature Getv V1 Strain In Micementioning

confidence: 99%

“…In previous studies, we have isolated a new GETV strain, V1 strain, from pregnant sows had abortions, and have demonstrated the V1 strain of GETV had a strong cytopathic effect and higher proliferation titer of vaccine (Zhou et al, 2020). In this study, we utilized mice model to investigate the infectiousness and pathogenicity of mature GETV.…”

Section: Introductionmentioning

confidence: 99%

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Structure of Infective Getah Virus at 2.8 Å-resolution Determined by Cryo-EM

Wang

Zhou

Liu

et al. 2021

Preprint

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Getah virus (GETV) is a mosquito-borne pathogen that can cause a mild illness and reproductive losses in animals. Although antibodies to GETV have been found in humans, there are no reports of clinical symptom associated with GETV. However, antivirals or vaccine against GETV is still unavailable due to lack of knowledge of the structure of GETV virion. Here, we present the structure of mature GETV at a resolution of 2.8 Å with capsid protein, envelope glycoproteins E1 and E2. Glycosylation and S-acylation sites in E1 and E2 are identified. The surface-exposed glycans demonstrated their impact on the viral immune evasion and host cell invasion. The S-acylation sites strongly stabilize the virion. In addition, a cholesterol and phospholipid molecule are observed in transmembrane hydrophobic pocket, together with two more cholesterols surround the pocket. These structural information are helpful for structure-based antivirals and vaccine design.

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Section: Infectiousness Of Mature Getv V1 Strain In Micementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structure of Infective Getah Virus at 2.8 Å-resolution Determined by Cryo-EM

Wang

Zhou

Liu

et al. 2021

Preprint

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“…The occurrence of abortion is related to female animals and closely related to the semen quality of male animals ( 13 , 14 ). The reproductive effects of GETV on male animals have not been reported ( 15 , 16 ). Therefore, we explored the effects of the Getah virus on testis, epididymis, and sperm in mice to reveal the reproductive effects of GETV in male mice.…”

Section: Introductionmentioning

confidence: 99%

Getah Virus Infection Rapidly Causes Testicular Damage and Decreases Sperm Quality in Male Mice

Zhang

et al. 2022

Front. Vet. Sci.

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Getah virus (GETV) is a zoonotic arbovirus that can cause infection in many animals. It can cause pyrexia and reproductive losses in animals. The objective of the study was to explore the effects of GETV on male reproductive ability. Male mice were injected with 100 × TCID50/0.1 ml in a volume of 100-μL GETV in their hindquarter muscle, resulting in decreased semen quality and testicular histopathological changes, and the virus was detected in the testes. At 0.5 dpi (day post-infection), male mice showed decreased sperm density, motility, and decreased serum testosterone concentration, an increased sperm malformation rate, vacuoles in spermatogonial cells/spermatocytes in spermatogenic tubules, and the highest virus copies in testis. At 2 dpi, the sperm density and motility reached the lowest value of 3.99 × 106/ml and 62.03%, and the malformation rate reached 43.67%. At 28 dpi, the sperm indexes of the experimental group gradually approached that of the control group, but there were still significant differences. Since then, histopathological changes have worsened, with the most severe histopathological changes at 7 dpi and gradual recovery. Up to 14 dpi, the virus was detected by qRT-PCR and immunohistochemistry, which showed that the virus was only present in the testicular interstitium. GETV infection can rapidly enter the testis of mice and reduce the semen quality of mice, which needs to be paid attention to in the prevention and control of GETV.

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“…In swine, GETV infection often result in the abortion of piglets(Xing et al 2020). Recently, GETV was detected in a commercial live-attenuated vaccine preparation aimed at preventing infections with Porcine reproductive and respiratory syndrome virus (PRRSV)(Zhou et al 2020). The contamination occurred during the manufacturing and might result in PPRSV vaccination-related GETV infections(Zhou et al 2020).…”

mentioning

confidence: 99%

“…Recently, GETV was detected in a commercial live-attenuated vaccine preparation aimed at preventing infections with Porcine reproductive and respiratory syndrome virus (PRRSV)(Zhou et al 2020). The contamination occurred during the manufacturing and might result in PPRSV vaccination-related GETV infections(Zhou et al 2020). Pigs immunized with these commercial live PPRSV-vaccines might be a source for further GETV spread to other vertebrate species that likely serve as reservoir host for GETV.Thus, there is a great need for a safe and effective GETV vaccine.…”

mentioning

confidence: 99%

Emerging animal viruses in China, from discovery to control measures

Miao¹

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Isolation and phylogenetic analysis of Getah virus from a commercial modified live vaccine against porcine reproductive and respiratory syndrome virus

Cited by 9 publications

References 13 publications

Structure of Infective Getah Virus at 2.8 Å-resolution Determined by Cryo-EM

Structure of Infective Getah Virus at 2.8 Å-resolution Determined by Cryo-EM

Getah Virus Infection Rapidly Causes Testicular Damage and Decreases Sperm Quality in Male Mice

Emerging animal viruses in China, from discovery to control measures

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