Isolation and phenotypical characterization of mesenchymal stem cells from human fetal thymus

Rzhaninova, A. A.; Gornostaeva, Svetlana; Goldshtein, D. V.

doi:10.1007/s10517-005-0231-4

Cited by 30 publications

(20 citation statements)

References 14 publications

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“…1). Examples of tissues where MSCs have been characterized include, but are not limited to: mesodermalbone marrow, trabecular bone, synovium, cartilage, fat, muscle, and tonsil (Trabecular bone: Noth et al, 2002;Synovium: De Bari et al, 2001;Djouad et al, 2005;Cartilage: Hiraoka et al, 2006;Fat: Zuk et al, 2001;Sekiya et al, 2004;Muscle: Mauro, 1961;Lee et al, 2000;Nesti et al, 2008;Tonsil: Janjanin et al, 2008), endodermal-thymus (Thymus: Rzhaninova et al, 2005), ectodermal-skin, hair follicle, dura mater, and dental pulp (mesoderm and ectoderm-derived) (Skin: Shih et al, 2005; Hair follicle: Cotsarelis et al, 1990;Sieber-Blum and Grim, 2004;Dura mater: Petrie et al, 2008;Dental pulp: Perry et al, 2008), prenatal and perinatal tissues-umbilical cord, umbilical cord blood, and placenta (Umbilical cord: Sarugaser et al, 2005; Umbilical cord blood: Lee et al, 2004a;Placenta: Yen et al, 2005). Stem cells derived from these tissues and organs are commonly referred to as MSCs; however, in this review we will use the term MSCs to specifically refer to those derived from bone marrow.…”

Section: Tissue Sources For Mesenchymal Stem Cellsmentioning

confidence: 99%

Regulation of stemness and stem cell niche of mesenchymal stem cells: Implications in tumorigenesis and metastasis

Kuhn

Tuan

2009

Journal Cellular Physiology

240

184

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Human mesenchymal stem cells (MSCs) derived from adult tissues have been considered a candidate cell type for cell-based tissue engineering and regenerative medicine. These multipotent cells have the ability to differentiate along several mesenchymal lineages and possibly along non-mesenchymal lineages. MSCs possess considerable immunosuppressive properties that can influence the surrounding tissue positively during regeneration, but perhaps negatively towards the pathogenesis of cancer and metastasis. The balance between the naïve stem state and differentiation is highly dependent on the stem cell niche. Identification of stem cell niche components has helped to elucidate the mechanisms of stem cell maintenance and differentiation. Ultimately, the fate of stem cells is dictated by their microenvironment. In this review, we describe the identification and characterization of bone marrow-derived MSCs, the properties of the bone marrow stem cell niche, and the possibility and likelihood of MSC involvement in cancer progression and metastasis.

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Section: Tissue Sources For Mesenchymal Stem Cellsmentioning

confidence: 99%

Regulation of stemness and stem cell niche of mesenchymal stem cells: Implications in tumorigenesis and metastasis

Kuhn

Tuan

2009

Journal Cellular Physiology

240

184

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“…In the search for more optimal donor site substitutes, MPCs have been isolated from a number of adult tissues, including adipose tissue [8], synovial membrane [9], muscle [10,11], dermis [11], skin [12], trabecular bone [13], thymus [14], salivary gland [15], palatine tonsil [16] and, most recently, nasal mucosa [17]. …”

Section: Introductionmentioning

confidence: 99%

Human ethmoid sinus mucosa: a promising novel tissue source of mesenchymal progenitor cells

et al. 2014

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IntroductionThe identification of new progenitor cell sources is important for cell-based tissue engineering strategies, understanding regional tissue regeneration, and modulating local microenvironments and immune response. However, there are no reports that describe the identification and isolation of mesenchymal progenitor cells (MPCs) from paranasal sinus mucosa, and compare the properties of MPCs between tissue sources within the sinonasal cavity. We report here the identification of MPCs in the maxillary sinus (MS) and ethmoid sinus (ES). Furthermore, we contrast these MPCs in the same individuals with MPCs from two additional head and neck tissue sources of the inferior turbinate (IT) and tonsil (T).MethodsThese four MPC sources were exhaustively compared for morphology, colony-forming potential, proliferation capability, immunophenotype, multilineage differentiation potential, and ability to produce soluble factors.ResultsMS-, ES, IT-, and T-MPCs showed similar morphologies and surface phenotypes, as well as adipogenic, osteogenic, and chondrogenic differentiation capacity by immunohistochemistry and qRT-PCR for defined lineage-specific genes. However, we noted that the colony-forming potential and proliferation capability of ES-MPCs were distinctly higher than other MPCs. All MPCs constitutively, or upon stimulation, secrete large amounts of IL-6, IL-8, IL-10, IFN-γ, and TGF-β. After stimulation with TNF-α and IFN-γ, ES-MPCs notably demonstrated significantly higher secretion of IL-6 and IL-10 than other MPCs.ConclusionsES-MPCs may be a uniquely promising source of MPCs due to their high proliferation ability and superior capacity toward secretion of immunomodulatory cytokines.

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“…MPCs were initially identified and isolated from bone marrow (BM) and are characterized by the expression of a number of cell surface markers [3-5]. Based on their clonogenic and multipotent differentiation activities, to date, MPCs have been isolated from a number of adult tissues, including trabecular bone [6], fat [7,8], synovium [9,10], skin [11], thymus [11,12], periodontal ligament [13], as well as prenatal and perinatal sources such as umbilical cord blood [14], umbilical cord [15], palatine tonsil [16], and placenta [17]. The diversity of sources facilitates MPC accessibility, but also raises questions about possible phenotypic and functional discrepancies that must be addressed for their clinical use.…”

Section: Introductionmentioning

confidence: 99%

Activin A expression regulates multipotency of mesenchymal progenitor cells

et al. 2010

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IntroductionBone marrow (BM) stroma currently represents the most common and investigated source of mesenchymal progenitor cells (MPCs); however, comparable adult progenitor or stem cells have also been isolated from a wide variety of tissues. This study aims to assess the functional similarities of MPCs from different tissues and to identify specific factor(s) related to their multipotency.MethodsFor this purpose, we directly compared MPCs isolated from different adult tissues, including bone marrow, tonsil, muscle, and dental pulp. We first examined and compared proliferation rates, immunomodulatory properties, and multidifferentiation potential of these MPCs in vitro. Next, we specifically evaluated activin A expression profile and activin A:follistatin ratio in MPCs from the four sources.ResultsThe multidifferentiation potential of the MPCs is correlated with activin A level and/or the activin A:follistatin ratio. Interestingly, by siRNA-mediated activin A knockdown, activin A was shown to be required for the chondrogenic and osteogenic differentiation of MPCs. These findings strongly suggest that activin A has a pivotal differentiation-related role in the early stages of chondrogenesis and osteogenesis while inhibiting adipogenesis of MPCs.ConclusionsThis comparative analysis of MPCs from different tissue sources also identifies bone marrow-derived MPCs as the most potent MPCs in terms of multilineage differentiation and immunosuppression, two key requirements in cell-based regenerative medicine. In addition, this study implicates the significance of activin A as a functional marker of MPC identity.

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Isolation and phenotypical characterization of mesenchymal stem cells from human fetal thymus

Cited by 30 publications

References 14 publications

Regulation of stemness and stem cell niche of mesenchymal stem cells: Implications in tumorigenesis and metastasis

Regulation of stemness and stem cell niche of mesenchymal stem cells: Implications in tumorigenesis and metastasis

Human ethmoid sinus mucosa: a promising novel tissue source of mesenchymal progenitor cells

Activin A expression regulates multipotency of mesenchymal progenitor cells

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