Isolation and pathogenicity identification of bacterial pathogens in bleached disease and their physiological effects on the red macroalga Gracilaria lemaneiformis

Liu, Xiaojuan; Chen, Yang; Zhong, Min; Chen, Weizhou; Lin, Qiang; Du, Hong

doi:10.1016/j.aquabot.2018.11.002

Cited by 12 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The red macroalgae G. lemaneiformis is not only important commercially as a food resourse, but also effective in decreasing eutrophication 29 , 30 . Thus, G. lemaneiformis has been cultivated on a large scale in China's southeast coastal areas, particularly Nan’ao Island, Nanri Island, and Lianjiang County, making it the third largest economic macroalgae after Laminaria and Porphyra 31 .…”

Section: Introductionmentioning

confidence: 99%

Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis

Pei

Aslam

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Macroalgae host various symbionts on their surface, which play a critical role in their growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation of the epiphytic bacterial communities (EBC) composition of red macroalga Gracilariopsis lemaneiformis at different geographic locations and environmental factors (i.e., nitrogen and phosphorus), which shape the EBC composition of G. lemaneiformis. The composition and structure of EBC were characterized using high throughput sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. The results revealed that epiphytic bacteria varied significantly among three different geographic locations in China, i.e., Nan’ao Island (NA), Lianjiang County (LJ), and Nanri Island (NR). Redundancy analysis (RDA) showed that the relative abundance of Bacteroidetes, Firmicutes, Verrucomicrobia, and Epsilonbacteraeota at NR were strongly positively correlated with total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO3-N), and dissolved inorganic nitrogen (DIN), but negatively correlated with nitrite nitrogen (NO2-N). The relative abundance of Cyanobacteria at NA and LJ were strongly positively correlated with NO2-N, but negatively correlated with TN, TP, NO3-N, and DIN. Besides, the Mantel test results indicated that the EBC composition was significantly correlated with these environmental factors, which was also confirmed by Spearman correlation analysis. Thus, environmental factors such as NO3-N and DIN play a key role in the community composition of epiphytic bacteria on G. lemaneiformis. This study provides important baseline knowledge on the community composition of epiphytic bacteria on G. lemaneiformis and shows correlation between different epiphytic bacteria and their surrounding environmental factors.

show abstract

Section: Introductionmentioning

confidence: 99%

Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis

Pei

Aslam

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was also indicated that the reallocation or differential regulation of amino acids synthesis resulted in the degradation of phycobiliprotein, especially phycoerythrin under stress (Liu et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that N deficiency EFFECTS OF NITROGEN ON GRACILARIOPSIS LEMANEIFORMIS could lead to the degradation of algal pigments, for example, phycoerythrin (Msanne et al 2012, Valledor et al 2014, Liefer et al 2018, Sirikhachornkit et al 2018. It was also indicated that the reallocation or differential regulation of amino acids synthesis resulted in the degradation of phycobiliprotein, especially phycoerythrin under stress (Liu et al 2019).…”

Section: Effects Of Nitrogen On Gracilariopsis Lemaneiformismentioning

confidence: 99%

Physiological effects of nitrogen deficiency and recovery on the macroalga Gracilariopsis lemaneiformis (Rhodophyta)

Liu

Wen

Chen

et al. 2019

Journal of Phycology

Self Cite

View full text Add to dashboard Cite

Algal metabolites are the most promising feedstocks for bio‐energy production. Gracilariopsis lemaneiformis seems to be a good candidate red alga for polysaccharide production, especially relating to the agar production industry. Nitrogen deficiency is an efficient environmental pressure used to increase the accumulation of metabolites in algae. However, there are no studies on the physiological effects of G. lemaneiformis in response to nitrogen deficiency and its subsequent recovery. Here we integrated physiological data with molecular studies to explore the response strategy of G. lemaneiformis under nitrogen deficiency and recovery. Physiological measurements indicated that amino acids and protein biosynthesis were decreased, while endogenous NH4+ and soluble polysaccharides levels were increased under nitrogen stress. The expression of key genes involved in these pathways further suggested that G. lemaneiformis responded to nitrogen stress through up‐regulation or down‐regulation of genes related to nitrogen metabolism, and increased levels of endogenous NH4+ to complement the deficiency of exogenous nitrogen. Consistent with the highest accumulation of soluble polysaccharides, the gene encoding UDP‐glucose pyrophosphorylase, a molecular marker used to evaluate agar content, was dramatically up‐regulated more than 4‐fold compared to the relative expression of actin after 4 d of nitrogen recovery. The present data provide important information on the mechanisms of nutrient balance in macroalgae.

show abstract

“…G. lemaneiformis, is commercially importance red macroalgae. This is not only a source of human food and abalone fodder, but also an ideal material to restore the eutrophication of water body 29,30 . G. lemaneiformis has been cultivated on a large scale in southeast coastal areas of China particularly in Nan'ao Island, Nanri Island and Lianjiang County, and has quickly become the third largest economic macroalgae after Laminaria and Porphyra 31 .…”

Section: Also Reportedmentioning

confidence: 99%

Environmental Factors Shape the Epiphytic Bacterial Communities of Gracilariopsis Lemaneiformis

Pei

Aslam

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Macroalgae host variety of symbionts on their surface which play critical role in their growth and development processes. Nevertheless, the complete understanding of this interaction of epiphytic bacteria and host algae is still in its infancy. This study comprehensively analyses epiphytic bacterial communities composition of red macroalga Gracilariopsis lemaneiformis and environmental factors such as nitrogen and phosphorus which shape the composition of epiphytic bacterial communities of G. lemaneiformis and variation of epiphytic bacterial communities composition at different geographical location. The composition and structure of epiphytic bacterial communities were characterized using high throughput sequencing data of the V3-V4 hypervariable region of 16S rRNA gene amplicon sequencing. The epiphytic bacterial communities composition data revealed that epiphytic bacteria varied significantly among three different geographic locations i) Nan’ao Island (NA) (ii) Lianjiang County (LJ) and iii) Nanri Island (NR) in China. Redundancy analysis (RDA) showed that the relative abundance of Bacteroidetes, Firmicutes, Verrucomicrobia and Epsilonbacteraeota at NR were strongly positively correlated with total nitrogen (TN), total phosphorus (TP), nitrate nitrogen (NO3-N), and dissolved inorganic nitrogen (DIN), whereas negatively correlated with nitrite nitrogen (NO2-N). In addition, the relative abundance of cyanobacteria at NA and LJ were strongly positively correlated with NO2-N, whereas negatively correlated with TN, TP, NO3-N, and DIN. Furthermore, the results of Mantel test indicated that the epiphytic bacterial communities composition is significantly correlated with these environmental factors, which are also proved by Pearson correlation analysis. In conclusion, it is proposed that environmental factors such as NO3-N and DIN play key role in the communities composition of epiphytic bacteria in G. lemaneiformis. Our study provides important baseline knowledge for the communities composition of epiphytic bacteria in G. lemaneiformis and their correlation among themselves as well as with their surrounding environmental factors.

show abstract

Isolation and pathogenicity identification of bacterial pathogens in bleached disease and their physiological effects on the red macroalga Gracilaria lemaneiformis

Cited by 12 publications

References 35 publications

Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis

Environmental factors shape the epiphytic bacterial communities of Gracilariopsis lemaneiformis

Physiological effects of nitrogen deficiency and recovery on the macroalga Gracilariopsis lemaneiformis (Rhodophyta)

Environmental Factors Shape the Epiphytic Bacterial Communities of Gracilariopsis Lemaneiformis

Contact Info

Product

Resources

About