Isolation and partial characterization of a growth factor from human cementum

Yonemura, Kensuke; Narayanan, A. Sampath; Miki, Yoshiharu; Page, Roy C.; Okada, Hiroshi

doi:10.1016/0169-6009(92)90806-o

Cited by 39 publications

(19 citation statements)

References 29 publications

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“…Other studies reported the identification of phosphopro- teins in cementum or tooth roots (Glimcher and Lefteriou, 1989;McCurdy et al, 1990McCurdy et al, , 1992. More recent investigations have used biochemical assays and histochemical and immunohistochemical approaches to identify and localize NCPs and other organic components during odontogenesis, and it has now been demonstrated that OPN, BSP, osteonectin (SPARC), fibronectin, tenascin, cementum-derived attachment protein (CAP), and growth factors are all present to some degree in cementum or at the tooth root surface (Thesleff et al, 1981;Miki et al, 1987;Bronckers et al, 1989;Nishimura et al, 1989;Slavkin et al, 1989;Vaino et al, 1989;McAllister et al, 1990;Olson et al, 1991;Sasano et al, 1992;Yonemura et al, 1992;Somerman et al, 1993;MacNeil et al, 1994;McKee and Nanci, 1995;Pitaru et al, 1995). Here, we have advanced these earlier studies by using transmission electron microscopy together with high-resolution immunocytochemistry and lectin cytochemistry to definitively identify specific, glycosylated organic constituents in both acellular and cellular cementum, and to localize these constituents within fine, structural features of these two types of cementum.…”

Section: Organic Composition Of Cementummentioning

confidence: 97%

Extracellular matrix in tooth cementum and mantle dentin: Localization of osteopontin and other noncollagenous proteins, plasma proteins, and glycoconjugates by electron microscopy

1996

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Section: Organic Composition Of Cementummentioning

confidence: 97%

Extracellular matrix in tooth cementum and mantle dentin: Localization of osteopontin and other noncollagenous proteins, plasma proteins, and glycoconjugates by electron microscopy

1996

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“…Laminin and fibronectin can also function as adhesion proteins, together with tenascin (137), bone sialoprotein (38, 142), osteopontin (25), and a 55‐kDa cementum‐attachment protein (196, 263). The presence of other bioactive proteins, such as enamel‐like proteins (235, 234), osteonectin/SPARC (201), and mitogenic factors (157, 269), have also been reported in the cementum. In addition to these proteins, cementoblasts synthesize and secrete several glycosaminoglycans (such as chondroitin‐4‐sulfate, chondroitin‐6‐sulfate and dermatan sulfate, and collagen fibrils), which are present in the cemento–dentinal junction (88, 264, 265).…”

Section: Development Of the Periodontiummentioning

confidence: 99%

Regeneration of periodontal tissues: cementogenesis revisited

Zeichner‐David¹

2006

Periodontology 2000

117

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“…The differences in the onset and time course of collagen I expression between the periodontal bone and some primary osteoblast cultures is most likely due to the variability of phe- (Rodan, 1981;McCulloch et al, 1987McCulloch et al, , 1989McCulloch and Heersche, 1988 (Pavlin et al, 1998(Pavlin et al, , 2001a (TakanoYamamoto et al, 1994), BSP (Lekic et al, 1996), and osteonectin (Tung et al, 1985)-are also expressed in cementoblasts (MacNeil et al, 1995;D'Errico et al, 1997), while other markers, such as cementum-derived attachment protein and cementumderived growth factor, are specifically associated with cementoblasts (Arzate et al, 1992;Yonemura et al, 1992Yonemura et al, , 1993Pitaru et al, 1993;Liu et al, 1997;Metzger et al, 1998;Saito and Narayanan, 1999). However, there are several differences between bone and cementum, the most obvious being the lack of vascularity and innervation in cementum.…”

Section: (I) Introductionmentioning

confidence: 99%

Effect of Mechanical Loading On Periodontal Cells

Pavlin

Gluhak-Heinrich²

2001

Critical Reviews in Oral Biology & Medicine

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Mechanical loading is an important regulatory factor in alveolar bone homeostasis, and plays an essential role in maintaining the structure and mass of the alveolar processes throughout lifetime. A better understanding of the cellular and molecular responses of periodontal cells is a prerequisite for further improvements of therapeutic approaches in orthodontics, periodontal and alveolar bone repair and regeneration, implantology, and post-surgical wound healing. The purpose of this review is to provide an insight into some cell culture and animal models used for studying the effects of mechanical loading on periodontal cells, and into the recent developments and utilization of new in vivo animal models. There has been an increased awareness about the need for improvement and development of in vivo models to supplement the widely used cell culture models, and for biological validation of in vitro results, especially in the light of evidence that developmental models may not always reflect bone homeostasis in an adult organism. Due to the limitations of in vivo models, previous studies on mechanical regulation of alveolar bone osteoblasts and cementoblasts mostly focused on proliferative responses, rather than on the stimulation of cell differentiation. To address this problem, we have recently characterized and implemented a mouse osteoinductive tooth movement model for studying mechanically induced regulation of osteoblast-and cementoblast-associated genes. In this model, a defined and reproducible mechanical osteogenic loading is applied during a time course of up to two weeks. Regulation of gene expression in either wild-type or transgenic animals is assessed by a relative quantitative measurement of the level of target mRNAs directly within the subpopulations of periodontal cells. To date, results demonstrate a defined temporal pattern of cellspecific gene regulation in periodontal osteoblasts mechanically stimulated to differentiate and deposit bone matrix. The responses of osteoblast-associated genes to mechanical loading were 10-to 20-fold greater than the increase in the numbers of these cells, indicating that the induction of differentiation and an increase of cell function are the primary responses to osteogenic loading. The progression of the osteoblast phenotype in the intact mouse periodontium was several-fold faster compared with that in cultured cells, suggesting that the mechanical signal may be targeting osteoblast precursors in the state of readiness to respond to an environmental challenge, without the initial proliferative response. An early response of alkaline phosphatase and bone sialoprotein genes was detected after 24 hrs of treatment, followed by a concomitant stimulation of osteocalcin and collagen I between 24 and 48 hrs, and deposition of osteoid after 72 hrs. Although cementoblasts constitutively express biochemical markers similar to those of osteoblasts, distinct responses of osteocalcin, collagen I, and bone sialoprotein genes to mechanical loading were observed in the ...

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Isolation and partial characterization of a growth factor from human cementum

Cited by 39 publications

References 29 publications

Extracellular matrix in tooth cementum and mantle dentin: Localization of osteopontin and other noncollagenous proteins, plasma proteins, and glycoconjugates by electron microscopy

Extracellular matrix in tooth cementum and mantle dentin: Localization of osteopontin and other noncollagenous proteins, plasma proteins, and glycoconjugates by electron microscopy

Regeneration of periodontal tissues: cementogenesis revisited

Effect of Mechanical Loading On Periodontal Cells

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