Isolation and partial characterization of a 30 kDa adhesin from Paracoccidioides brasiliensis

Andreotti, Patrícia Ferrari; Silva, Juliana Leal Monteiro da; Bailão, Alexandre Mello; Soares, Célia Maria de Almeida; Benard, Gil; Soares, Christiane Pienna; Mendes-Giannini, María José Soares

doi:10.1016/j.micinf.2005.02.005

Cited by 64 publications

(68 citation statements)

References 35 publications

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“…brasiliensis uses a sequence of different mechanisms to establish successful infections, beginning with the first contact with host cells and extending through the later stages of disease. 48,49 The reduced initial adhesion observed in this study suggests that the Pb14-3-3 gene products strongly influence the initial adhesion, as reported by Andreotti et al, 2005, 27 Da Silva et al, 2013 28 and De Oliveira et al, 2015, 50 but the requirement for these proteins was bypassed following extended contact with the host cell.…”

Section: Discussionsupporting

confidence: 76%

“…[29][30][31] Previous studies focused on elucidating the function of the 14-3-3 protein in the pathogenesis of P. brasiliensis. 27,28 Those studies were limited to the use of the recombinant 14-3-3 protein and the anti-14-3-3 antibody, and they therefore indirectly explored the role of 14-3-3 without manipulating gene expression. This is the first report to use a strain of P. brasiliensis with reduced transcript and protein levels of 14-3-3 to elucidate the role of this protein in host-pathogen interactions and in the biology and virulence of P. brasiliensis.…”

Section: Discussionmentioning

confidence: 99%

“…The ability to adhere is a biological phenomenon that is widely distributed and shared by many pathogens, enabling the colonization of their respective habitats. 15,16 Some adhesins have been described for P. brasiliensis, such as gp43, 17,18 glyceraldehyde-3-phosphate dehydrogenase, 19 triose phosphate isomerase, 20 enolase, 21,22 a 32-Kda hydrolase, 23,24 malate synthase, 25 isocitrate lyase 26 and the 14-3-3 protein (30 KDa) 27,28 ; this last adhesin is the focus of the present study.…”

Section: Introductionmentioning

confidence: 95%

“…27 Additionally, immunocytochemical studies using both in vitro and in vivo models have demonstrated the ubiquitous distribution (cytosol and cell membrane) of the 14-3-3 protein in the yeast form of P. brasiliensis. A significant increase in the amount of 14-3-3 was observed in the fungal cell wall during interaction with pneumocytes and in an experimental acute infection in mice.…”

Section: Introductionmentioning

confidence: 99%

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Decreased expression of 14-3-3 inParacoccidioides brasiliensisconfirms its involvement in fungal pathogenesis

et al. 2016

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Decreased expression of 14-3-3 inParacoccidioides brasiliensisconfirms its involvement in fungal pathogenesis

et al. 2016

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“…brasiliensis to adhere to and invade epithelial cells (Mendes-Giannini et al, 2008), and the adhesion phenomenon is variable depending on the isolate (Andreotti et al, 2005;Hanna et al, 2000). Adhesion of P. brasiliensis to host tissues is a crucial step in the establishment of PCM.…”

Section: R Peres Da Silva and Othersmentioning

confidence: 99%

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Silva

Matsumoto

Braz

et al. 2011

Journal of Medical Microbiology

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Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, one of the most important systemic fungal diseases in Latin America. This initiates in lung tissue and can subsequently disseminate to other tissues. Clinical manifestations range from localized forms to disseminated disease that can progress to lethality, probably depending on the relationships among the virulence of the fungus, the immune response and the ability to interact with the surface structures and invade epithelial cells and mononuclear cells of the host. It is generally regarded as a multifocal disease, with oral lesions as the prominent feature. The aim of this study was to evaluate P. brasiliensis yeast infection in normal oral keratinocytes (NOKs). The differential expression of mRNAs and proteins was also determined when the fungus was placed in contact with the cell in order to characterize differentially expressed genes and proteins during P. brasiliensis infection. After contact with NOKs, the fungus appeared to induce alterations in the cells, which showed cellular extensions and cavitations, probably resulting from changes in the actin cytoskeleton seen at 5 and 8 h after infection. Levels of protein expression were higher after reisolation of the fungus from infected NOK culture compared with culture of the fungus in medium. The analysis identified transcripts related to 19 proteins involved in different biological processes. Transcripts were found with multiple functions including induction of cytokines, protein metabolism, alternative carbon metabolism, zinc transport and the stress response during contact with NOKs. The proteins found suggested that the yeast was in a stress situation, as indicated by the presence of RDS1. Nevertheless, the yeast seemed to be proliferating and metabolically active, as shown by the presence of a proteasome, short-chain acetylator, glucosamine-6-phosphate isomerase and ADP/ATP carrier transcripts. Additionally, metabolic pathways may have been activated in order to eliminate toxic substances from the cell as a zinc transporter was detected, which is a potential target for the development of future drugs. INTRODUCTIONParacoccidioides brasiliensis is a thermodimorphic pathogenic fungus, the aetiological agent of paracoccidioidomycosis (PCM), an endemic disease geographically limited to Latin America and one of the most prevalent human deep systemic mycoses found in Brazil, Colombia and Venezuela (San-Blas et al., 2002). The transition from mycelium at 25 u C to yeast at 37 u C is essential for P. brasiliensis to establish the disease (Franco et al., 1997). P. brasiliensis may be inhaled and, once in the lungs, the fungus initiates a process of morphological transition into the pathogenic yeast form (San-Blas et al., 2002).PCM has a multiplicity of clinical presentations, from cutaneous to systemic forms, and can attack various tissues, especially the lungs (Benard & Franco, 2007). Oral mucosal lesions are commonly multiple, with a granular, mulberry-like surface and microulcerations, and ...

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Simultaneous estimation of E‐ and Z‐isomers of guggulsterone in rabbit plasma using liquid chromatography tandem mass spectrometry and its application to pharmacokinetic study

Bhatta

Kumar

Chhonker

et al. 2011

Biomedical Chromatography

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A sensitive and selective liquid chromatography/tandem mass spectrometric method was developed for simultaneous determination of E- and Z-guggulsterone isomers (antihyperlipidemic drug) in rabbit plasma. Both the isomers were resolved on a Symmetry-Shield C(18) (5 µm, 4.6 × 150 mm) column, using gradient elution comprising a mobile phase of methanol, 0.5% v/v formic acid and acetonitrile. With dexamethasone as internal standard, plasma samples were extracted by an automated solid-phase extraction method using C(18) cartridges. Detection was performed by electrospray ionization in multiple reaction monitoring (MRM) in positive mode. The calibration curve was linear over the concentration range of 1.56-200 ng/mL (r(2) ≥ 0.998) for both analytes. The intra-day and inter-day accuracy and precision were within -0.96 to 4.12 (%bias) and 2.73 to 8.00 (%RSD) respectively. The analytes were stable after three freeze-thaw cycles. The method was successfully applied to study steriospecific pharmacokinetics of E- and Z-guggulsterone in NZ rabbit.

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Isolation and partial characterization of a 30 kDa adhesin from Paracoccidioides brasiliensis

Cited by 64 publications

References 35 publications

Decreased expression of 14-3-3 inParacoccidioides brasiliensisconfirms its involvement in fungal pathogenesis

Decreased expression of 14-3-3 inParacoccidioides brasiliensisconfirms its involvement in fungal pathogenesis

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Simultaneous estimation of E‐ and Z‐isomers of guggulsterone in rabbit plasma using liquid chromatography tandem mass spectrometry and its application to pharmacokinetic study

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