Isolation and partial characterization of crispacin A, a cell-associated bacteriocin produced by Lactobacillus crispatus JCM 2009

Tahara, Takatsugu; Kanatani, Kazuo

doi:10.1111/j.1574-6968.1997.tb10255.x

Cited by 26 publications

(12 citation statements)

References 12 publications

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“…The biochemical characteristics of our isolates are comparable with vaginal Lactobacillus isolated from other studies (Alpay et al, 2002;Aslim and Kilic, 2006;Anukam and Reid, 2007;Martin et al, 2008;Pascual et al, 2008a;Gordana et al, 2011 (Pascual et al, 2008a;Tahara and Kanatani, 1997;Bogovic-Matijasic et al, 1998;Alpay et al, 2002;Razak et al, 2011). Similarly, BLIS produced by our isolates were able to inhibit the test organism (GBS).…”

Section: Discussionsupporting

confidence: 72%

Effect of Bacteriocin-like Inhibitory Substances Produced by <i>Vaginal Lactobacilli</i> on Group B Streptococcus

Alajekwu¹,

Ike²

2017

Nig. J. Biotechnol

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Reduction of vaginal Lactobacillus population leads to overgrowth of opportunistic organisms such as Streptococcus agalactiae (Group B Streptococcus, GBS), which causes life threatening neonatal infections. The activities of bacteriocin-like inhibitory substances (BLIS) produced by Lactobacillus species isolated from the vagina of pregnant women were tested on GBS. Crude Bacteriocins were produced from seven Lactobacillus isolates using de Man Rogosa Sharpes media. Partial purification was done using ammonium sulphate saturation (90%) and dialysis methods. The antibacterial effects of the crude and partially purified BLIS were carried out by disc Agar diffusion method. The BLIS from strains R5 and L2 gave the highest and lowest inhibition zone diameter (IZD) of 16.9 mm and 12.1 mm, respectively, for the crude extracts and 18.5 mm and 14.2 mm, respectively, for the partially purified extracts. All the BLIS had their strongest activity at 30°C and decreased with increase in temperature. BLIS from strains L3 and L4 lost their activities at 100°C. The activities of all the BLIS were higher at acidic pH. The IZD of the combined crude extract was 19.06 mm, while that of the combined partially purified extracts was 21.07 mm. The combined BLIS had higher activity than each of the single compound. There were no statistical difference between the sensitivities of the partially purified BLIS and the crude BLIS (P > 0.05). The findings of this study show that BLIS produced by vaginal Lactobacilli spp. can be used as probiotics for the control of GBS and that combining the BLIS may be more effective.

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Section: Discussionsupporting

confidence: 72%

Effect of Bacteriocin-like Inhibitory Substances Produced by <i>Vaginal Lactobacilli</i> on Group B Streptococcus

Alajekwu¹,

Ike²

2017

Nig. J. Biotechnol

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“…It has been noticed that 60–70% of the bacteriocins recovered in this study were cell-wall associated peptides bound to the producer cells while the rest of the inhibitory peptides were secreted extracellularly into the liquid media. Cell-associated bacteriocins produced by lactic acid bacteria had been reported previously [44] , [45] . Hence, this class of bacteriocins can be recovered from producer cells grown in liquid media.…”

Section: Discussionmentioning

confidence: 84%

Variable Characteristics of Bacteriocin-Producing Streptococcus salivarius Strains Isolated from Malaysian Subjects

Barbour

Philip

2014

PLoS ONE

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BackgroundSalivaricins are bacteriocins produced by Streptococcus salivarius, some strains of which can have significant probiotic effects. S. salivarius strains were isolated from Malaysian subjects showing variable antimicrobial activity, metabolic profile, antibiotic susceptibility and lantibiotic production.Methodology/Principal FindingsIn this study we report new S. salivarius strains isolated from Malaysian subjects with potential as probiotics. Safety assessment of these strains included their antibiotic susceptibility and metabolic profiles. Genome sequencing using Illumina’s MiSeq system was performed for both strains NU10 and YU10 and demonstrating the absence of any known streptococcal virulence determinants indicating that these strains are safe for subsequent use as probiotics. Strain NU10 was found to harbour genes encoding salivaricins A and 9 while strain YU10 was shown to harbour genes encoding salivaricins A3, G32, streptin and slnA1 lantibiotic-like protein. Strain GT2 was shown to harbour genes encoding a large non-lantibiotic bacteriocin (salivaricin-MPS). A new medium for maximum biomass production buffered with 2-(N-morpholino)ethanesulfonic acid (MES) was developed and showed better biomass accumulation compared with other commercial media. Furthermore, we extracted and purified salivaricin 9 (by strain NU10) and salivaricin G32 (by strain YU10) from S. salivarius cells grown aerobically in this medium. In addition to bacteriocin production, S. salivarius strains produced levan-sucrase which was detected by a specific ESI-LC-MS/MS method which indicates additional health benefits from the developed strains.ConclusionThe current study established the bacteriocin, levan-sucrase production and basic safety features of S. salivarius strains isolated from healthy Malaysian subjects demonstrating their potential for use as probiotics. A new bacteriocin-production medium was developed with potential scale up application for pharmaceuticals and probiotics from S. salivarius generating different lantibiotics. This is relevant for the clinical management of oral cavity and upper respiratory tract in the human population.

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“…There have been other reports of possible, cell-associated bacteriocins (Banerjee et al, 2013; Barbour and Philip, 2014; Burmølle et al, 2006; Daba et al, 1994; Dang and Lovell, 2016; Tahara and Kanatani, 1997; Yang et al, 1992), but these prior cases are unlikely to represent contact-dependent killing systems. In most of these cases, significant killing activity was also found in cell-free supernatants and the bacteriocins were typically easily detected in supernatants, suggesting they are primarily secreted into the environment and not exclusively cell-associated as with CdzC and CdzD.…”

Section: Discussionmentioning

confidence: 99%

Contact-dependent killing by Caulobacter crescentus via cell surface-associated, glycine zipper proteins

García-Bayona

Guo

Laub

2017

eLife

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Most bacteria are in fierce competition with other species for limited nutrients. Some bacteria can kill nearby cells by secreting bacteriocins, a diverse group of proteinaceous antimicrobials. However, bacteriocins are typically freely diffusible, and so of little value to planktonic cells in aqueous environments. Here, we identify an atypical two-protein bacteriocin in the α-proteobacterium Caulobacter crescentus that is retained on the surface of producer cells where it mediates cell contact-dependent killing. The bacteriocin-like proteins CdzC and CdzD harbor glycine-zipper motifs, often found in amyloids, and CdzC forms large, insoluble aggregates on the surface of producer cells. These aggregates can drive contact-dependent killing of other organisms, or Caulobacter cells not producing the CdzI immunity protein. The Cdz system uses a type I secretion system and is unrelated to previously described contact-dependent inhibition systems. However, Cdz-like systems are found in many bacteria, suggesting that this form of contact-dependent inhibition is common.DOI: http://dx.doi.org/10.7554/eLife.24869.001

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Isolation and partial characterization of crispacin A, a cell-associated bacteriocin produced by Lactobacillus crispatus JCM 2009

Cited by 26 publications

References 12 publications

Effect of Bacteriocin-like Inhibitory Substances Produced by <i>Vaginal Lactobacilli</i> on Group B Streptococcus

Effect of Bacteriocin-like Inhibitory Substances Produced by <i>Vaginal Lactobacilli</i> on Group B Streptococcus

Variable Characteristics of Bacteriocin-Producing Streptococcus salivarius Strains Isolated from Malaysian Subjects

Contact-dependent killing by Caulobacter crescentus via cell surface-associated, glycine zipper proteins

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