Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes.

Stremmel, Wolfgang; Strohmeyer, G; Borchard, F; Kochwa, Shaul; Berk, Paul D.

doi:10.1073/pnas.82.1.4

Cited by 310 publications

(197 citation statements)

References 37 publications

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“…That these proteins are similar and mediate FFA uptake has been clearly demonstrated. By immunofluorescence, an antibody raised in rabbits against the rat liver FABPpM bound specifically to the plasma membranes of cardiac myocytes, adipocytes, and jejunal enterocytes (12)(13)(14). This antibody also inhibited oleate uptake, noncompetitively and in a dosedependent fashion, in rat hepatocytes (9), adipocytes (12) and, in preliminary studies, in the gut (15).…”

Section: Discussionmentioning

confidence: 99%

“…More recently, we and others have shown that FFA uptake has the kinetic characteristics of a carrier-mediated process in hepatocytes (8,9) and adipocytes (10)(11)(12), and have isolated and characterized basic, 40-kD FABPpM from these and other tissues with high transmembrane fluxes of fatty acids (13,14,20). That these proteins are similar and mediate FFA uptake has been clearly demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…Forthe uptake studies myocytes were washed three times and resuspended (-1 X 106/ml) in Hanks' buffer containing 10 (20). After dilution (1:1) and centrifugation (2,500 g for 30 min at 40C) the supernatant was passed through an oleate agarose affinity column (14). After washing extensively with (14,20).…”

Section: Methodsmentioning

confidence: 99%

“…After dilution (1:1) and centrifugation (2,500 g for 30 min at 40C) the supernatant was passed through an oleate agarose affinity column (14). After washing extensively with (14,20). The assay was performed on samples subjected to SDS-PAGE (20).…”

Section: Methodsmentioning

confidence: 99%

“…Hence, the nature of the uptake process in cardiac myocytes remains uncertain. By contrast, studies from several laboratories, including our own, have documented that FFA uptake by hepatocytes (8,9) and adipocytes (10-12) is unequivocally carrier mediated, and that a 40-kD plasma membrane fatty acid binding protein (fPABPpM)' isolated from these tissues and from the gut (12)(13)(14) plays an essential role in fatty acid uptake (8,9,12,15). The aims of the present study were, therefore (a) to study the uptake ofa representative FFA,…”

Section: Introductionmentioning

confidence: 99%

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Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane fatty acid binding protein similar to that in liver, adipose tissue, and gut.

Sorrentino

Stump²,

Potter³

et al. 1988

J. Clin. Invest.

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185

102

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Uptake of V3Hjoleate by canine or rat cardiac myocytes is saturable, displays the countertransport phenomenon, and is inhibited by phloretin and trypsin. Cardiac myocytes contain a basic (pI 9.1) 40-kD plasma membrane fatty atid binding protein (FABPpM) analogous to those recently isolated from liver, adipose tissue, and gut, unrelated to the 12-14-kD cytosolic FAIRP in these same tissues. An antibody to rat liver FABPpM selectively inhibits specific uptake of [3Hfoleate by rat heart myocytes at 370C, but has no influence on nonspecific VHoleate uptake at 40C or on specific uptake of VHjglucose. Uptake of long-chain free fatty acids by cardiac muscle cells, liver, and adipose tissue and absorption by gut epithelial cells is a facilitated process mediated by identical or closely related plasma membrane FABPs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane fatty acid binding protein similar to that in liver, adipose tissue, and gut.

Sorrentino

Stump²,

Potter³

et al. 1988

J. Clin. Invest.

Self Cite

185

102

View full text Add to dashboard Cite

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Transport, metabolism and distribution space of octanoate in the perfused rat liver

Ferraresi-Filho

Ishii–Iwamoto

Bracht

1997

Cell Biochem. Funct.

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The scope of the present work was to investigate the metabolism and the passage of octanoate from albumin into the phospholipid bilayer of the plasma membrane and from thence into the cell space. The experiments were done in the isolated perfused rat liver with infusions of albumin and octanoate at various concentrations. Once steady-state conditions were attained, trace amounts of [1-14C]-octanoate, [131 I]-albumin and [3H]-water were injected simultaneously and the effluent perfusate was fractionated. The normalized dilution curves were used for model analysis. The model which gives the best fit to the experimental results and which also produces the most consistent parameters is one that presupposes a rapid distribution of octanoate into the cell membrane and a slow transfer from the cell membrane into the cytosol. The concentration dependence of the distribution between the membrane and the extracellular space is parabolic, suggesting that octanoate changes the properties of the cell membrane when present at higher concentrations. The passage from the cell membrane into the cell space is relatively slow and limits metabolic transformation partly or totally, depending on the octanoate concentration in the plasma membrane. The rapid transfer of octanoate from the albumin space into the plasma membrane corroborates previous measurements of the dissociation of the albumin-octanoate complex.

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Fatty acids as regulators of lipid metabolism

Wolfrum

Spener

2000

Eur. J. Lipid Sci. Technol.

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Fatty acids as regulators of lipid metabolismStructurally defined fatty acid species, which are the straight-chain monounsaturated and polyunsaturated and the branched-chain building blocks of dietary fats and oils, have the potential to regulate lipid metabolism. Focussing on the situation in rodents and in man we describe first the non-enzymic proteins that confer regulatory properties to fatty acids. These are the ligand activated receptors in the nuclei (peroxisome proliferator activated receptors, hepatic nuclear factor 4, liver-X-receptor), sterol regulatory element binding proteins and the soluble and membrane-bound transport proteins for fatty acids and derivatives (fatty acid binding proteins, acyl-CoA binding protein, fatty acid translocator, fatty acid translocator proteins). Then we follow the path of the dietary fatty acids from digestion to their ultimate fate in the cell and critically address their regulatory roles. Fatty acids and/or derivatives interact either directly with enzymes to affect activity, or with the nuclear transcription factors, or affect the stability of mRNAs encoding proteins involved in lipid metabolism. Knowledge of the effects of fatty acid species on the genetic machinery as a whole could become a starting point for individualization of nutritional needs.

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Isolation and partial characterization of a fatty acid binding protein in rat liver plasma membranes.

Cited by 310 publications

References 37 publications

Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane fatty acid binding protein similar to that in liver, adipose tissue, and gut.

Oleate uptake by cardiac myocytes is carrier mediated and involves a 40-kD plasma membrane fatty acid binding protein similar to that in liver, adipose tissue, and gut.

Transport, metabolism and distribution space of octanoate in the perfused rat liver

Fatty acids as regulators of lipid metabolism

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