Isolation and Partial Characterization of Lactic Acid Bacteria from the Gut Microbiota of Marine Fishes for Potential Application as Probiotics in Aquaculture

Alonso, Sergio Collado; Castro, M. Carmen; Berdasco, Margarita; Banda, Inés García de la; Moreno-Ventas, X.; Rojas, Alma Hernández de

doi:10.1007/s12602-018-9439-2

Cited by 62 publications

(35 citation statements)

References 38 publications

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“…This proves their ability in the bio-preservation of food and can be used as starter culture in the fermentation process under controlled conditions. Therefore, the isolation and characterization of LAB from different traditional fermented foods and products have gained research interest in recent years (Alonso et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Probiotic Properties of Lactic Acid Bacteria Isolated From Neera: A Naturally Fermenting Coconut Palm Nectar

et al. 2019

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Probiotic bacteria were isolated from different traditional fermented foods as there are several such foods that are not well explored for their probiotic activities. Hence, the present study was conducted to find the potential of lactic acid bacteria (LAB) as probiotics that were isolated from the sap extract of the coconut palm inflorescence – Neera, which is a naturally fermented drink consumed in various regions of India. A total of 75 isolates were selected from the Neera samples collected aseptically in the early morning (before sunrise). These isolates were initially screened for cultural, microscopic, and biochemical characteristics. The initial screening yielded 40 Gram-positive, catalase-negative isolates that were further subjected to acid – bile tolerance with resistance to phenol. Among 40 isolates, 16 survived screening using analysis of cell surface hydrophobicity, auto aggregation with adhesion to epithelial cells, and gastric–pancreatic digestion for gastrointestinal colonization. The isolates were also assessed for antimicrobial, antibiotic sensitivity, and anti-oxidative potential. The safety of these isolates was evaluated by their hemolytic and deoxyribonuclease (DNase) activities. Based on these results, seven isolates with the best probiotic attributes were selected and presented in this study. These LAB isolates, with 51.91–70.34% survival at low pH, proved their resistance to gastric conditions. The cell surface hydrophobicity of 50.32–77.8% and auto aggregation of 51.02–78.95% represented the adhesion properties of these isolates. All the seven isolates exhibited good antibacterial and antifungal activity, showing hydroxyl-scavenging activity of 32.86–77.87%. The results proved that LAB isolated from Neera exhibited promising probiotic properties and seem favorable for use in functional fermented foods as preservatives.

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Section: Introductionmentioning

confidence: 99%

Probiotic Properties of Lactic Acid Bacteria Isolated From Neera: A Naturally Fermenting Coconut Palm Nectar

et al. 2019

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“…Probiotics are viable cell preparations that have beneficial effects on the host's health, improving intestinal balance by producing nutrients, enhancing immune responses and improving water quality in aquaculture (Assefa & Abunna, 2018; Chauhan & Singh, 2019; Ringø, Van Doan, Lee, Soltani, et al., 2020; Ringø et al., 2020). In recent years, in order to reduce the uncontrollable usage of antimicrobials in aquaculture products, probiotics have been increasingly applied in aquaculture to prevent diseases and improve tolerance to high stocking densities (Alonso et al., 2019; Nawaz et al., 2018; Tang et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Effects of Clostridium butyricum on growth, digestive enzyme activity, antioxidant capacity and gut microbiota in farmed tilapia ( Oreochromis niloticus )

Zhang

Dong

Lai

et al. 2020

Aquaculture Research

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The effects of a commercial probiotic (Clostridium butyricum) on growth, gut microbiota, digestion‐related enzyme activity, antioxidant capacity and water quality in genetically improved, farmed tilapia (GIFT; Oreochromis niloticus) were assessed in a closed‐circuit, container culture system. A basal diet containing commercially available C. butyricum at 3.0 × 1010, 1.5 × 1011 and 3.0 × 1011 CFU/kg, and a control diet were fed to tilapia fries for 90 days. Growth performances and water quality were improved by probiotic treatment. Activities of digestive enzymes (amylase, lipase and trypsin) were improved, especially with 1.5 × 1011 CFU/kg (p < 0.05). After feeding for 45 days and 90 days, antioxidant capacity in the liver, spleen and head kidney, and the plasma immunity in the treatment groups improved. The analysis of 16S rRNA indicated that C. butyricum did not significantly increase its abundance in the hindgut, but could modulate gut microbial communities and significantly increase functions relating to nitrogen metabolism, phosphorylation and proteinases. These results support the administration of C. butyricum, especially at 1.5 × 1011 CFU/kg. As a probiotic for high‐density tilapia, C. butyricum improves water quality, growth performance, antioxidant capacity and the immune response of GIFT under crowding stress.

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“…Besides, probiotics could promote digestion and enhance nutrient absorption by altering the intestinal microflora, resulting in better growth performance (Balcazar et al, 2006; Falcinelli et al, 2015). Lactic acid bacteria (LAB) and Bacillus species are among the most commonly used probiotic candidates (Cao et al, 2011; Banerjee and Ray, 2017; Alonso et al, 2018; Yi et al, 2018). LAB strains that have been used for fish pathogens control usually include the genera Lactobacillus , Leuconostoc , Streptococcus , and Pediococcus (Perez-Ramos et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

A New Isolate of Pediococcus pentosaceus (SL001) With Antibacterial Activity Against Fish Pathogens and Potency in Facilitating the Immunity and Growth Performance of Grass Carps

Gong

et al. 2019

Front. Microbiol.

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Probiotic-feeding continues to be a promising strategy to control the bacterial pathogens in aquaculture. A new Pediococcus pentosaceus strain (SL001) was isolated from 1000s of soil samples, which exhibited wide antimicrobial spectrum of against fish pathogens, involving Aeromonas hydrophila , Aeromonas veronii , Aeromonas sobria , Edwardsiella tarda , Lactococcus garvieae , and Plesiomonas shigelloide . The challenge test against A. hydrophila showed that the survival rate of SL001-supplemented group was significantly higher than that of control group ( P < 0.05). Moreover, SL001 could stably colonize in gut of grass carp and increased mucus-secreting goblet cells and extended intestinal villi could be observed in SL001-supplemented group ( P < 0.05). Feeding with SL001 supplemented diet could significantly enhance the growth rate ( P < 0.05) and markedly affect gut microbiota structure of grass carps, resulting in reduced potential pathogens and increased potential probiotics. Furthermore, feeding grass carps with SL001 caused the up-regulated expression of insulin-like growth factor (IGF-1 and IGF-2) and down-regulated expression of myostatin (MSTN-1 and MSTN-2) ( P < 0.05), which probably also account for the increased growth rate of SL001-fed group. Meanwhile, relative mRNA expression levels of immune-related genes in liver, spleen, and head kidney were analyzed in grass carps after feeding for 30 days with SL001 supplemented diets. In all three immune organs, the expression levels of immunoglobulin M (IgM) and complement 3 (C3) were significantly increased ( P < 0.05), whereas the interleukin-8 (IL-8) was down-regulated ( P < 0.05). Besides, whole genome sequencing revealed several probiotics properties of SL001, including organic acid synthesis, bacteriocin synthesis (coagulin), superoxide dismutase, and digestive enzymes. In conclusion, P. pentosaceus SL001 which could enhance immunity and promoter growth rate of grass carps, is prospective to be used as a dietary probiotic in freshwater fish aquaculture.

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Isolation and Partial Characterization of Lactic Acid Bacteria from the Gut Microbiota of Marine Fishes for Potential Application as Probiotics in Aquaculture

Cited by 62 publications

References 38 publications

Probiotic Properties of Lactic Acid Bacteria Isolated From Neera: A Naturally Fermenting Coconut Palm Nectar

Probiotic Properties of Lactic Acid Bacteria Isolated From Neera: A Naturally Fermenting Coconut Palm Nectar

Effects of Clostridium butyricum on growth, digestive enzyme activity, antioxidant capacity and gut microbiota in farmed tilapia ( Oreochromis niloticus )

A New Isolate of Pediococcus pentosaceus (SL001) With Antibacterial Activity Against Fish Pathogens and Potency in Facilitating the Immunity and Growth Performance of Grass Carps

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