Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds

Moreira, Renato de Azevedo; Castelo-Branco, Camil; Monteiro, A. C. O.; Tavares, Ricardo O.; Beltramini, Leila Maria

doi:10.1016/s0031-9422(97)00753-x

Cited by 55 publications

(57 citation statements)

References 18 publications

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“…lectin is similar to those of Trichosanthes anguina seeds, 45,000 Da (Anuradha and Bhide, 1999) Artocarpus integrifolia, 46,000 Da, and Maclura pomifera, 44,000 Da (Moreira et al, 1998).…”

Section: Molecular Weightsupporting

confidence: 56%

Purification and Partial Characterization of a Lectin from the Fresh Leaves of Kalanchoe crenata (Andr.) Haw

Kuku

Eretan²

2004

BMB Reports

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A haemagglutinating protein from the saline extracts of Kalanchoe crenata leaves, which agglutinate all human blood types, was purified to homogeneity by ion-exchange chromatography on a DEAE-Cellulose column followed by gel filtration on a Sephadex G-100 column. The purified protein showed one band, both in non-denaturing PAGE and SDS-PAGE. The M r that was determined by SDS-PAGE was 44,000 Da and that estimated from gel filtration was 47,000. Treatment of the haemagglutinating protein with 5 mM EDTA diminished the haemagglutinating activity to 50% of the original level. The addition of divalent cations, 10 mM Mg 2+ , 10 mM Mn 2+ , or 10 mM Ba 2+ , totally restored and enhanced the activity. The protein showed maximum activity over the 3-7 pH range and was heat-resistant. It was also a glycoprotein containing about 1.5% carbohydrate.

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“…lectin is similar to those of Trichosanthes anguina seeds, 45,000 Da (Anuradha and Bhide, 1999) Artocarpus integrifolia, 46,000 Da, and Maclura pomifera, 44,000 Da (Moreira et al, 1998).…”

Section: Molecular Weightsupporting

confidence: 56%

Purification and Partial Characterization of a Lectin from the Fresh Leaves of Kalanchoe crenata (Andr.) Haw

Kuku

Eretan²

2004

BMB Reports

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“…Lima et al (2002) and Teixeira et al (2007) succeeded using natural exudates gums from Anacardium occidentale and Spondias purpurea, respectively to purify lectins. From some seed galactomannans (Moreira et al, 1998, GarrosRosa et al, 2006, Seshagirirao et al, 2005 new matrices showed the ability to isolate galactosebinding lectins with different efficiencies. The studies performed with exudates gums have compared the diverse formulations to form the affinity matrix while, until now, the endospermic studies have cited only one preparation for each galactomannan.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Caesalpinia pulcherrima endospermic gum as affinity matrices for galactose-binding lectins interaction

Braga

Sá

Ribeiro

et al. 2011

Braz. arch. biol. technol.

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“…The only four gum sources that are used as affinity ligands for the isolation of a few galactose-specific lectins are the Arabic gum (Fujita et al, 1975), the guar gum (Goldstein et al, 1976;Appakuttan et al, 1977;Komath et al, 1996;Cavada et al, 1998;Sampaio et al, 1998;Seshagirirao et al, 1999), Adenanthera pavonia galactomannan (Moreira et al, 1998), and quite recently, cashew gum (Lima et al, 2002). Among them, the cross-linked guar gum matrix is the most popularized affinity matrix.…”

Section: Introductionmentioning

confidence: 99%

Cross-linked Leucaena Seed Gum Matrix: An Affinity Chromatography Tool for Galactose-specific Lectins

Seshagirirao¹,

Leelavathi²,

Sasidhar³

2005

BMB Reports

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A cross-linked leucaena (Leucaena leucocephala) seed gum (CLLSG) matrix was prepared for the isolation of galactosespecific lectins by affinity chromatography. The matrix was evaluated for affinity with a known galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina). The matrix preparation was simple and inexpensive when compared to commercial galactose-specific matrices (i.e. about 1.5 US$/100 ml of matrix). The current method is also useful for the demonstration of the affinity chromatography technique in laboratories. Since leucaena seeds are abundant and inexpensive, and the matrix preparation is easy, CLLSG appears to be a promising tool for the separation of galactose-specific lectins.

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Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds

Cited by 55 publications

References 18 publications

Purification and Partial Characterization of a Lectin from the Fresh Leaves of Kalanchoe crenata (Andr.) Haw

Purification and Partial Characterization of a Lectin from the Fresh Leaves of Kalanchoe crenata (Andr.) Haw

Evaluation of Caesalpinia pulcherrima endospermic gum as affinity matrices for galactose-binding lectins interaction

Cross-linked Leucaena Seed Gum Matrix: An Affinity Chromatography Tool for Galactose-specific Lectins

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