Isolation and Optimization of PHB (Poly-β-hydroxybutyrate) Based Biodegradable Plastics from Chlorella vulgaris

Robert, Rebecca; Iyer, Priya

doi:10.4172/2155-6199.1000433

Cited by 9 publications

(4 citation statements)

References 3 publications

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“…This study covered qualitative analysis of PHB production with Sudan black dye. Black-stained colonies were considered as positive isolates for PHB production, which was matched with the findings of Robert and Iyer (2018) .…”

Section: Discussionmentioning

confidence: 99%

Community-forming traits play role in effective colonization of plant-growth-promoting bacteria and improved plant growth

Pathak,

Suman,

Sharma

et al. 2024

Front. Plant Sci.

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Community-forming traits (CFts) play an important role in the effective colonization of plant-growth-promoting bacterial communities that influence host plants positively by modulating their adaptive functions. In this study, by considering plant-growth-promoting traits (PGPts) and community-forming traits (CFts), three communities were constructed, viz., SM1 (PGPts), SM2 (CFts), and SM3 (PGPts+CFts). Each category isolates were picked up on the basis of their catabolic diversity of different carbon sources. Results revealed a distinctive pattern in the colonization of the communities possessed with CF traits. It was observed that the community with CFts colonized inside the plant in groups or in large aggregations, whereas the community with only PGPts colonized as separate individual and small colonies inside the plant root and leaf. The effect of SM3 in the microcosm experiment was more significant than the uninoculated control by 22.12%, 27.19%, and 9.11% improvement in germination percentage, chlorophyll content, and plant biomass, respectively. The significant difference shown by the microbial community SM3 clearly demonstrates the integrated effect of CFts and PGPts on effective colonization vis-à-vis positive influence on the host plant. Further detailed characterization of the interaction will take this technology ahead in sustainable agriculture.

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Section: Discussionmentioning

confidence: 99%

Community-forming traits play role in effective colonization of plant-growth-promoting bacteria and improved plant growth

Pathak,

Suman,

Sharma

et al. 2024

Front. Plant Sci.

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“…was described as a good candidate for PHB production; the yield was 21% of the cell dry weight in 15 days (Martins et al, 2017). Recently, PHB was detected in C. vulgaris by its extraction from the biomass using hot chloroform (Robert & Iyer, 2018) and it was quantified in a different study from 0.6 g of C. vulgaris biomass using mechanical (sonication) and chemical (NaClO) cell disruption methods, and the best yield was 37% (Setyorini & Dianursanti, 2021). Furthermore, C. vulgaris can be utilized in multiple production processes to make the process more sustainable and economical by utilizing the algal biomass completely without any production of algal waste.…”

Section: Polyhydroxyalkanoatesmentioning

confidence: 99%

“…Polyhydroxybutyrate (PHB) is another polyester that belongs to the PHAs family and is produced by bacteria (McAdam et al, 2020) and microalgae (Robert & Iyer, 2018a). However, PHA has better chemical and physical properties and is comparable to petroleum‐based plastics, whereas PHB has less elasticity, is very brittle, and its thermal properties are unsuitable for rigid products (Singh et al, 2015).…”

Section: Applicationsmentioning

confidence: 99%

New insights into Chlorella vulgaris applications

Al‐Hammadi,

Güngörmüşler

2024

Biotech & Bioengineering

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Environmental pollution is a big challenge that has been faced by humans in contemporary life. In this context, fossil fuel, cement production, and plastic waste pose a direct threat to the environment and biodiversity. One of the prominent solutions is the use of renewable sources, and different organisms to valorize wastes into green energy and bioplastics such as polylactic acid. Chlorella vulgaris, a microalgae, is a promising candidate to resolve these issues due to its ease of cultivation, fast growth, carbon dioxide uptake, and oxygen production during its growth on wastewater along with biofuels, and other productions. Thus, in this article, we focused on the potential of Chlorella vulgaris to be used in wastewater treatment, biohydrogen, biocement, biopolymer, food additives, and preservation, biodiesel which is seen to be the most promising for industrial scale, and related biorefineries with the most recent applications with a brief review of Chlorella and polylactic acid market size to realize the technical/nontechnical reasons behind the cost and obstacles that hinder the industrial production for the mentioned applications. We believe that our findings are important for those who are interested in scientific/financial research about microalgae.

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“…Therefore, understanding and exploring the biochemical profile of each microalgal species would help us to design a sustainable and cost-effective method to produce bio-fuels, bio-ethanol, protein, and omega fatty acid supplements. Within past few years, multiple studies have tried to explore various strategies and methods to explore and enhance either lipid accumulation, [12][13][14] or accumulation of various pigments, 15 or certain value-added secondary metabolites [16][17][18][19][20][21] in microalgal cells. However, only a handful of reports are available which compare entire biomass profile of various microalgal species at different growth phases.…”

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confidence: 99%

Bioprospecting of native algal strains with unique lipids, proteins, and carbohydrates signatures: A time dependent study

Samadhiya

Ghosh

Kashyap

et al. 2021

Env Prog and Sustain Energy

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Aim of this study is uncovering potential strains of microalgae from Indore, Madhya Pradesh situated in central India with a diverse climate for sustainable production of different bio-products. Assessing the entire biochemical profile is crucial to select species-specific metabolites for sustainability of algal bio-refinery processes. In the current study, seven algal species (C. proboscideum, Desmodesmus psuedocommunis, Asterarcys quadricellulare, Ettlia texensis, Pediludiela daitoensis, Coelastrella sp., Pectinodesmus sp.) were isolated and identified using Internal Transcribed Spacer (ITS) and 18 s rRNA sequences, and these species found to be from the Chlorophyta phylum. The highest biomass productivity (51.25 ± 1.96 μg/ml/day) with chlorophyll content (33.07 ± 0.63 μg/ml) was observed in A. quadricellulare on 21st day (P < 0.0001). Biochemical profiling of isolated species showed each species is unique in its biochemical composition (total lipids, carbohydrates, and proteins). Coelastrella sp. and Pectinodesmus sp. was found to be accumulating considerable quantities of (P < 0.0001) carbohydrates (303.3 ± 1.11 μg/mg dry cell weight) and lipids (212.67 ± 2.53 μg/mg per dry cell weight), respectively. Whereas P. daitoensis was found best (P < 0.0001) for protein production (205.78 ± 2.56 μg/mg dry cell weight), on 21st day. Highest total fatty acid content was detected in Pectinodesmus sp. (115.74 μg/ mg) on 21st day, which has shown good saturated fatty acid (SFA) and mono unsaturated fatty acid (MUFA) percentage, inflicting high quality biodiesel production. Confocal microscopy further supported the data. Assessing the biochemical profile at different phases of growth for each species therefore reduce the production time of biofuels as well as make this process much more cost-effective.

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Isolation and Optimization of PHB (Poly-β-hydroxybutyrate) Based Biodegradable Plastics from Chlorella vulgaris

Cited by 9 publications

References 3 publications

Community-forming traits play role in effective colonization of plant-growth-promoting bacteria and improved plant growth

Community-forming traits play role in effective colonization of plant-growth-promoting bacteria and improved plant growth

New insights into Chlorella vulgaris applications

Bioprospecting of native algal strains with unique lipids, proteins, and carbohydrates signatures: A time dependent study

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