Isolation and optimization of a novel thraustochytrid strain for DHA rich and astaxanthin comprising biomass as aquafeed supplement

Pawar, Pratik R.; Velani, Sneha; Kumari, Sujata; Lali, Arvind; Prakash, Gunjan

doi:10.1007/s13205-020-02616-4

Cited by 14 publications

(10 citation statements)

References 22 publications

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“…The answer to this question is because the concentration of astaxanthin complex in prawns may not be sufficient to completely inhibit the PPO activity in vivo. If astaxanthin complex-enriched feed is offered in prawn culture [72][73][74][75][76][77][78], there is a chance that the enriched astaxanthin in prawn tissues may inactivate prawn PPO, thereby preventing prawn melanosis after the harvest. Further research will test this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin Complex as an Antioxidant in Preventing Prawn Blackening or Melanosis

Vinothkumar¹,

Paterson²

2023

Health Risks of Food Additives - Recent Developments and Trends in Food Sector [Working Title]

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Melanosis or blackening in prawns is a common problem faced by seafood markets and industries. The formation of black spots decreases the quality of the prawn, as perceived by consumers, and thus reduces its commercial value. Controlling deterioration owing to blackening therefore poses a major challenge to food scientists. Prevention of prawn blackening is carried out mainly with the use of sulphites. The use of sulphites in food applications is a major problem because of their health-related problems in sensitive individuals. An alternative way of preventing melanosis in prawns without the use of chemicals, especially sulphites is necessary. Recently, the use of natural compounds in food applications is preferred by consumers over the use of artificial chemical additives. The main objective of this study is to perform a preliminary investigation on the inhibitory role of a natural antioxidant, astaxanthin complex against prawn melanosis as a natural alternative to chemicals.

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Section: Discussionmentioning

confidence: 99%

Astaxanthin Complex as an Antioxidant in Preventing Prawn Blackening or Melanosis

Vinothkumar¹,

Paterson²

2023

Health Risks of Food Additives - Recent Developments and Trends in Food Sector [Working Title]

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“…Some green algae, such as Chromochloris zoffingiensis, can accumulate astaxanthin, but the accumulated PUFAs is mainly C18 PUFAs (Liu et al, 2016;Mao et al, 2018). Therefore, some research groups are also attempting to co-produce long-chain PUFAs and astaxanthin in algae cells, making it more cost-effective for commercial production (Pawar et al, 2021).…”

Section: Analysis Of Research Hotspotsmentioning

confidence: 99%

Microalgal polyunsaturated fatty acids: Hotspots and production techniques

Chen

et al. 2023

Front. Bioeng. Biotechnol.

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Algae play a crucial role in the earth’s primary productivity by producing not only oxygen but also a variety of high-value nutrients. One such nutrient is polyunsaturated fatty acids (PUFAs), which are accumulated in many algae and can be consumed by animals through the food chain and eventually by humans. Omega-3 and omega-6 PUFAs are essential nutrients for human and animal health. However, compared with plants and aquatic sourced PUFA, the production of PUFA-rich oil from microalgae is still in the early stages of exploration. This study has collected recent reports on algae-based PUFA production and analyzed related research hotspots and directions, including algae cultivation, lipids extraction, lipids purification, and PUFA enrichment processes. The entire technological process for the extraction, purification and enrichment of PUFA oils from algae is systemically summarized in this review, providing important guidance and technical reference for scientific research and industrialization of algae-based PUFA production.

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“…Thraustochytrids are a promising source of fatty acids, notably omega-3 PUFAs such as eicosapentaenoic acid (EPA) and DHA (Raghukumar, 2008;Pawar et al, 2021). These fatty acids are important for aquatic ecological processes, and as essential nutrients for fishes (Song et al, 2015;Ye et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, DHA and EPA have garnered attention because of their pharmacological and nutritional properties, such as enhancing brain and retina development (Ratledge, 2004), hypertension reduction (Boyer-Diaz et al, 2020), anti-cardiovascular (Liao et al, 2022), and anticancer activities (Kalidasan et al, 2022). Hence the culture of PUFA-producing thraustochytrids has gained prominence for supply of PUFA-rich biomass and oils (Raghukumar, 2008;Raghukumar, 2017;Pawar et al, 2021). Currently, fish and fish oils are major sources for the production of DHA.…”

Section: Introductionmentioning

confidence: 99%

PUFA and carotenoid producing thraustochytrids and their anti-microbial and antioxidant activities

et al. 2023

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Thraustochytrids contribute to the microbiota of mangrove ecosystem, and they hold promise as a potential source of polyunsaturated fatty acids (PUFAs), antimicrobials and antioxidants for their application in pharmaceutical, aquaculture, and human health sectors. However, the thraustochytrids have not been properly studied in Indian mangrove ecosystems for their PUFAs and biological activities, and hence, the present study was carried to isolate the PUFAs-rich thraustochytrids for their pigments, antimicrobial and antioxidant properties. This work isolated and identified the thraustochytrids that are capable of producing PUFAs from decomposing leaves of mangroves at Pichavaram, southeast coast of India. Two predominant isolates were identified as Thraustochytrium sp. and Aurantiochytrium mangrovei based on morphological and molecular characteristics. Thraustochytrium sp., produced the biomass of 4.72 g L-1, containing total lipids of 42.36% and docosahexaenoic acid (DHA) of 32.69% of total lipids, whereas, A. mangrovei produced the biomass of 6.25 g L-1 containing total lipids of 49.81% and DHA of 44.71% of total lipids. Astaxanthin pigment accumulated up to 3.2 µg L-1 in A. mangrovei, whereas the pigment was not detected in Thraustochytrium sp. Further, the biomass extracted in organic solvents was tested for antibacterial activity against seven clinical pathogens along with positive control of ampicillin. Thraustochytrium sp., exhibited the highest antibacterial activity with the zone of inhibition of 78.77% against Staphylococcus aureus and the lowest (20.95%) against Klebsiella pneumonia. Thraustochytrium sp., also showed minimum inhibitory concentration (MIC) of 40 µg L-1 inhibiting the growth of S. aureus. The antioxidant activity of A. mangrovei was tested by using six assays and noted the highest free radical scavenging (87.37 ± 1.22%) and the lowest nitric oxide radical scavenging (75.12 ± 2.22%) activities. Hence, it is clear that the extracts of Thraustochytrium sp., and A. mangrovei are promising sources of lead compounds for biopharma and food industries.

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Isolation and optimization of a novel thraustochytrid strain for DHA rich and astaxanthin comprising biomass as aquafeed supplement

Cited by 14 publications

References 22 publications

Astaxanthin Complex as an Antioxidant in Preventing Prawn Blackening or Melanosis

Astaxanthin Complex as an Antioxidant in Preventing Prawn Blackening or Melanosis

Microalgal polyunsaturated fatty acids: Hotspots and production techniques

PUFA and carotenoid producing thraustochytrids and their anti-microbial and antioxidant activities

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