“…Genomic DNA from fin clippings was isolated using conventional salt‐extraction protocol as outlined in Aljanabi and Martinez (). Nine species‐specific microsatellite loci were employed for genotyping the C. striata , which consisted of six microsatellite markers (CS774A41, CS1‐A05, CSI‐B07, CSI‐C07, CSI‐E11, CSI‐E12, CS1‐F05) described previously (Jamsari, Tan, & Siti‐Azizah, ) and three novel markers (CS774A19, CS774A21, CS774A41) developed herein to complement existing ones (Table ). The latter were isolated from a microsatellite‐enriched DNA library constructed using the 5′‐anchored primer protocol (Brachet, Jubier, Richard, Jung‐Muller, & Frascaria‐Lacoste, ; Fisher, Gardner, & Richardson, ; Kinsuat & Kumar, ), where firstly, a degenerate oligonucleotide 5′‐KKVRVRV(guanine‐adenine, GA) 10 –3′(where K: G/T, V: G/C/A and R: G/A) was used to polymerase chain reaction (PCR)‐amplify regions containing a target motif, followed by cloning of the fragments using TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA) with blue/white screening.…”