Isolation and Morphological Identification of Fresh Water Green Algae from Organic Farming Habitats in Korea

Kim, Minjeong; Shim, Chang-Su; Kim, Yong-Ki; Hong, Sung-Jun; Park, Jong-Ho; Han, Eun‐Jung; Jee, Hyeong-Jin; Yun, Jong-Chul; Kim, Suk-Chul

doi:10.11625/kjoa.2014.22.4.743

Cited by 7 publications

(7 citation statements)

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“…The Chlorella strain used in this study was Chlorella fusca CHK0059 isolated from an organic rice paddy field before being cultured to purify in our laboratory [ 21 ]. The culture of chlorella was prepared by adding 5 ml of chlorella exclusive culture medium (F & B Nature Co. Ltd., Korea) modified with Bold’s basal media and BG11 media [ 21 ] to 8 L of commercial mineral water, and then inoculating uncontaminated C. fusca using “the chlorella farmer’s self-light incubator” at the farmer’s greenhouse in Nonsan. After inoculation, artificial light was irradiated over the chlorella at more than 2,000 lux, approximately equal to sunlight at 28~30°C, while air was blown over 5 L/min using a home bubble generator.…”

Section: Methodsmentioning

confidence: 99%

Effect of the MicroalgaChlorella fuscaCHK0059 on Strawberry PGPR and Biological Control of Fusarium Wilt Disease in Non-Pesticide Hydroponic Strawberry Cultivation

Kim

Shim

2020

J. Microbiol. Biotechnol.

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The purpose of this study was to identify strawberry wilt pathogens and evaluate the efficacy of Chlorella fusca CHK0059 for improving plant growth and suppressing Fusarium wilt. We identified 10 isolates of wilt pathogens of non-pesticide Seolhyang strawberry plant, including Fusarium oxysporum f. sp. fragariae , using morphological and molecular analysis. On the 15 th day after 0.4% CHK0059 treatment, the plant height of the untreated control strawberry plants was significantly greater than that of the CHK0059-treated strawberry plants. After 85 days, both treatments showed a similar tendency regarding the height of the strawberry plants. However, the thickness of strawberry leaves treated with the CHK0059 was found to be 1 mm thicker than that of the untreated control. The flowering percentage of the CHK0059 plants was also 40.2% higher on average than that of the untreated control. The chlorophyll content of strawberry leaves treated with the CHK0059 was also, on average, 6.63% higher than that of the untreated control. After 90 days of the CHK0059 treatment, the incidence of Fusarium wilt in the CHK0059-treated plants had reduced by 9.8% on average compared to the untreated control. The population density of F. oxysporum f. sp. fragariae was also reduced by approximately 86.8% in the CHK0059-treated plants by comparison to the untreated control at 70 days after treatment. The results indicate that the microalga C. fusca CHK0059 is an efficient biological agent for improving strawberry plant growth and suppressing Fusarium wilt disease in organic strawberries.

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Section: Methodsmentioning

confidence: 99%

Effect of the MicroalgaChlorella fuscaCHK0059 on Strawberry PGPR and Biological Control of Fusarium Wilt Disease in Non-Pesticide Hydroponic Strawberry Cultivation

Kim

Shim

2020

J. Microbiol. Biotechnol.

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“…It has also been found that Chlorella extracts contain cytokinin, which could be the reason for the increase in photosynthetic biomass [13]. A morpho-biometric study was conducted on lettuce seedlings by monitoring their chlorophyll, carotenoid, and total protein levels after application of chlorella extracts, foliar sprays, and root drenches [14,15]. According to the results, Chlorella extract significantly increased the dry matter, chlorophyll, carotenoid, and protein contents in lettuce seedlings [14].…”

Section: Resultsmentioning

confidence: 99%

Chlorella vulgaris improves growth of Abrometiella scapigera, Abrometiella brevifolia and Abrometiella chloranta and reduces root attack by nematodes

Domenico Prisa

2024

GSC Adv. Res. Rev.

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Research objective: The aim of this study is to investigate the effects of Chlorella extract as a biofertiliser on the growth of Abrometiella spp. seedlings and to assess whether it has a repellent effect against nematodes Materials and Methods: The experiments, which began in November 2024, were conducted in the CREA-OF greenhouses in Pescia (PT), Tuscany, Italy (43°54′N 10°41′E) on Abrometiella scapigera, Abrometiella brevifolia and Abrometiella chloranta. The plants were placed in ø12 cm pots; 30 plants per thesis, divided into 3 replicates of 10 plants each. The experimental groups were: i) group without algae, irrigated with water and previously fertilised substrate; ii) group with Chlorella vulgaris, previously fertilised substrate. On 2 April 2024, plant height, leaves number, vegetative weight, root weight, root lenght, number of new plantlets, chlorophyll content, leaf area, control of the nematodes Meloidogyne javanica and Meloidogyne incognita were measured. Results and Discussion: The experiment showed that the use of Chlorella vulgaris extracts can improve the vegetative and root growth of Abrometiella scapigera, Abrometiella brevifolia and Abrometiella chloranta plants grown in pots. The treatment also led to an increase in plant height, number of leaves, length of root hairs, leaf area and number of new seedlings. The trial also showed that the use of Chlorella in plant growing media can also increase the photosynthesis rate (SPAD index) and significantly reduce the presence of nematodes, in particular Meloidogyne javanica and Meloidogyne incognita. According to some researchers, the influence of Chlorella extract on cell metabolisms is mainly due to the physiological effect of major and minor nutrients, amino acids, vitamins, and plant growth regulators on cellular metabolisms in treated plants, which increase crop yields and growth. Chlorella plants have very high photosynthesis rates, dozens of times higher than other plants. The main reason why Chlorella can grow rapidly is because it contains Chlorella Growth Factor (CGF), which is quite rich in nucleoproteins, nucleic acids, ribonucleic acids (RNA), deoxyribonucleic acids (DNA), vitamins, amino acids, polysaccharides, complex protein bodies, enzymes, glycoproteins, plant hormones, etc. Its ability to promote plant growth may be due to this factor. Conclusions: Chlorella extract significantly increased Abrometiella seedling height, leaf number and leaf area. In particular, Abrometiella seedlings treated with chlorella extract developed better root system, chlorophyll a increased significantly and the root length and number of new seedlings also improved significantly. Based on the above results that Chlorella extracts contribute to the growth and development of Abrometiella plants, we can conclude that with the treatment of Chlorella extracts, the application of chemical fertilisers in the production of succulents such as Abrometiella can be reduced.

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“…The Chlorella vulgaris was separately cultivated in Bold Basal culture medium (BBM) according to the method of Kim et al 30 Briefly, the strain of Chlorella vulgaris was placed in a control room at constant temperature (30 °C) for 6 days in a shaking incubator at the speed of 180 rpm, while the pH of the culture medium was 6.0. After seven days, the Chlorella was harvested when its dry biomass concentration was about 180 g/L in 7.5 L bench scale fermenter 29 .…”

Section: Methodsmentioning

confidence: 99%

“…The condensed ethanol extract was dried at ambient temperature before being placed in an airtight amber vial and kept at 4 °C in a desiccator. While using the extract, it was first solubilized with dimethyl sulfoxide (DMSO) and then Chlorella extracts were diluted with distilled water to a concentration of 0.4 mg/L 30 . The leaves of pepper seedlings were sprayed with extracts solution, whereas the untreated control plants were sprayed with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Effects of Chlorella extracts on growth of Capsicum annuum L. seedlings

Tian

Khan

Zheng

et al. 2022

Sci Rep

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The long-term application of chemical fertilizers has caused to the farmland soil compaction, water pollution, and reduced the quality of vegetable to some extent. So, its become a trend in agriculture to find new bio-fertilizers. Chlorella extract is rich in amino acids, peptides, nucleic acids, growth hormones, potassium, calcium, magnesium, iron, zinc ions, vitamin E, B1, B2, C, B6, folic acid, free biotin and chlorophyll. Chlorella extract can promote biological growth, mainly by stimulating the speed of cell division, thereby accelerating the proliferation rate of cells and playing a role in promoting plant growth. Whether Chlorella extract can be used to improve the growth of pepper (Capsicum annuum), needs to be verified. In current study, a pepper variety 'Chao Tian Jiao' was used as experiment material, by determining the changes of the related characteristics after spraying the seedlings with Chlorella extract, and its effect on growth of Capsicum annuum plants was investigated. The results showed that the Chlorella extract significantly increased plant height of pepper seedlings (treatment: 32.2 ± 0.3 cm; control: 24.2 ± 0.2 cm), stem diameter (treatment: 0.57 ± 0.02 cm; control: 0.41 ± 0.03 cm) and leaf area (treatment: 189.6 ± 3.2 cm2; control: 145.8 ± 2.5 cm2). Particularly, the pepper seedlings treated with Chlorella extract, developed the root system in better way, significantly increased the chlorophyll a, and the activities of SOD, POD and CAT enzymes were also improved significantly. Based on our results, we can speculate that it is possible to improve the growth of Capsicum annuum seedlings and reduce the application of chemical fertilizers in pepper production by using Chlorella extract.

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Isolation and Morphological Identification of Fresh Water Green Algae from Organic Farming Habitats in Korea

Cited by 7 publications

References 0 publications

Effect of the MicroalgaChlorella fuscaCHK0059 on Strawberry PGPR and Biological Control of Fusarium Wilt Disease in Non-Pesticide Hydroponic Strawberry Cultivation

Effect of the MicroalgaChlorella fuscaCHK0059 on Strawberry PGPR and Biological Control of Fusarium Wilt Disease in Non-Pesticide Hydroponic Strawberry Cultivation

Chlorella vulgaris improves growth of Abrometiella scapigera, Abrometiella brevifolia and Abrometiella chloranta and reduces root attack by nematodes

Effects of Chlorella extracts on growth of Capsicum annuum L. seedlings

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