Isolation and molecular identification of Aspergillus flavus and the study of its potential for malathion biodegradation in water

Derbalah, Aly; Khattab, Ismael; Allah, Moustafa Saad

doi:10.1007/s11274-020-02869-4

Cited by 11 publications

(9 citation statements)

References 34 publications

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“…They examined dense hyphae of A. flavus that produced conidia under SEM. In contrary to present study, (Derbalah, Khattab, & Saad, 2020) reported the morphological description of A. flavus on Czapek Dox agar. They described flat and granular colonies with yellow green to dark green color on Czapek Dox agar.…”

Section: Discussioncontrasting

confidence: 85%

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Seerat

Akram

Qureshi

et al. 2022

Microscopy Res & Technique

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Maize (Zea mays L.) is considered as one of the main cereals, used as a source of food, forage, and processed products. The loss of maize productivity is reported due to effect on roots, stalks, ears, and kernels mainly caused by many fungi. Among these fungal pathogens of maize, Aspergillus flavus (A. flavus) are the most prevalent that produces highly toxigenic aflatoxins that are highly carcinogenic to the consumers.The present study is confined to isolate and characterize the A. flavus from maize seeds for accurate identification that can be helpful for determination and management of aflatoxins in maize crop. Eighty stored seed samples of maize were collected from warehouses where seeds are stored for food and feeding purposes. For the isolation of A. flavus, Potato Dextrose Agar was used. Isolated fungi were identified macro and microscopically using light microscope and scanning electron microscope.A total of 212 Aspergillus isolates were identified based on macro-morphological and micro-morphological characteristics. The results showed that A. flavus colonies were granular, flat with yellow-green to deep yellow-green colony color having a white border and compact, spherical spore heads. Rapid rate of growth was observed maturing in about 3-5 days. In microscopic features, A. flavus have apically swollen conidiophores with various conidia bearing cells in long and dry chains. Spherical conidial heads were split into several columns ranging 300-400 μm in diameter. This will be helpful for farmers, researchers and traders in future for correct identification of sources of aflatoxins. Research Highlights• Maize seed samples were collected from Pothohar region of Pakistan.• The fungi were isolated on PDA.• Aspergillus flavus was identified macro-morphologically by observing growth rate, colony color and texture.• Furthermore, these fungi were identified micro-morphologically by using light and scanning electron microscope.• The 212 Aspergillus flavus strains were isolated and identified.

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Section: Discussioncontrasting

confidence: 85%

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Seerat

Akram

Qureshi

et al. 2022

Microscopy Res & Technique

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“…Many different types of esterase have been observed in bacteria and fungi causing degradation of OP insecticides 4,7,8 . Liu et al 35 .…”

Section: Resultsmentioning

confidence: 99%

“…Chlorpyrifos, malathion and dimethoate are among the first 10 ranked insecticides as per market estimate 6 . Hydrolytic degradation of malathion, 8 dimethoate 26,35 and chlorpyrifos 37 has been reported. However, Liu et al 35 .…”

Section: Resultsmentioning

confidence: 99%

“…Derbalah et al 8 . observed that degradation of malathion by A. flavus increased directly with applied insecticide concentration up to 5 mg L −1 causing removal of about 20% parent compound during the first 10 days.…”

Section: Resultsmentioning

confidence: 99%

“…The high biodegradability and effective target neurotoxicity have made OP insecticides the most preferred choice in agricultural pest control practices 3,4 . However, continuous and overuse of OP compounds have induced pollution of several ecosystems around the world 5–8 …”

Section: Introductionmentioning

confidence: 99%

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Evaluating a preparation of malathion‐tolerant Aspergillus niger MRU01 for accelerated removal of four organophosphorus insecticides

Mohapatra

Rath

Mohapatra

2021

J of Chemical Tech & Biotech

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BACKGROUND Microbial degradation of organophosphorus (OP) insecticides has emerged as an efficient and cost‐effective technology for removal of pesticide residues from soil. Malathion‐tolerant Aspergillus (Aspergillus niger MRU01) was developed, mass cultured, harvested, mixed with coir pith at 3% on dry weight basis, shade‐dried aseptically to a 5% moisture level and stored for 60 days. The growth, metabolic activity and insecticide removal efficiencies of the preparation were evaluated at 15‐day intervals. RESULTS No significant change in the growth performance was observed up to 45 days of storage, after which viability decreased. The activities of carbohydrate enzymes (cellulase, invertase and amylase) and protein and carbohydrate contents of the preparation, however, did not significantly change during the experimental period. The preparation showed accelerated activities of esterases and phosphatases after treatment with malathion (500 μmol L−1), parathion (470 μmol L−1), chlorpyrifos (260 μmol L−1) and dimethoate (680 μmol L−1). The highest activity of the esterase and phosphatase enzymes was observed with malathion and the minimum with parathion treatment. Nearly 70% of the applied malathion was removed from the medium by the preparation during 5 days of incubation followed by dimethoate (68%), chlorpyrifos (58%) and parathion (54%). CONCLUSIONS The fungal preparation was highly effective in removing malathion as well as the other three OP insecticides. A storage period of 45 days may be considered ideal for maintaining the preparation in the active state, and is recommended for field application after evaluating its efficacy under field conditions. © 2021 Society of Chemical Industry

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Pesticides: Types, Toxicity and Recent Updates on Bioremediation Strategies

Deolikar

Pandit

Jadhav

et al. 2021

Environmental Challenges and Solutions

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Isolation and molecular identification of Aspergillus flavus and the study of its potential for malathion biodegradation in water

Cited by 11 publications

References 34 publications

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Light and scanning electron microscopic characterization of aflatoxins producing Aspergillus flavus in the maize crop

Evaluating a preparation of malathion‐tolerant Aspergillus niger MRU01 for accelerated removal of four organophosphorus insecticides

Pesticides: Types, Toxicity and Recent Updates on Bioremediation Strategies

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