Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran

Abstract

Introduction. Keratinophilic fungi are an important group of fungi that live in soil. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz. Materials and Methods. A total of 196 soil samples from 43 parks were collected. Isolation of the fungi was performed by hair bait technique. The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis. ITS region of ribosomal DNA was amplified and the PCR products were sequenced. Results. 411 isolates from 22 genera were identified. Fusarium (23.8%), Chrysosporium (13.13%), Acremonium (12.65%), Penicillium (12.39%), Microsporum gypseum (1.94%), Bionectria ochroleuca (1.21%), Bipolaris spicifera (1.21%), Scedosporium apiospermum (0.82%), Phialophora reptans (0.82%), Cephalosporium curtipes (0.49%), Scedosporium dehoogii (0.24%), Ochroconis constricta (0.24%), Nectria mauritiicola (0.49%), Chaetomium (0.49%), Scopulariopsis (0.24%), Malbranchea (0.24%), and Tritirachium (0.24%) were the most important isolates. Most of the fungi were isolated from the soils with the PH range of 7 to 8. Conclusion. Our study results showed that many keratinophilic fungi isolated from the parks soil are important for public health and children are an important group at a high risk of being exposed to these fungi.

Effect of Soil Physicochemical Parameters and Seasonal Variations on the Occurrence of Keratinophilic Fungi of Murtala Amusement Park in Minna, Niger State, Nigeria

“…In the present study, all the keratinophilic fungi isolated during the dry and rainy season were from soil samples with pH range between 6.559 to 6.960. This finding has confirmed the studies of Pakshir et al [2] who in their study isolated keratinophilic fungi from soils with pH between six and nine. Many researchers had stated that keratinophilic fungi could not be found in the soils with low pH levels 3-4.5 [2].…”

“…This finding has confirmed the studies of Pakshir et al [2] who in their study isolated keratinophilic fungi from soils with pH between six and nine. Many researchers had stated that keratinophilic fungi could not be found in the soils with low pH levels 3-4.5 [2]. However, some researchers such as Korniłłowicz-Kowalska and Bohacz [32] isolated keratinophilic fungi from samples of strongly acidic soils (pH KCl 3.36; 4.06; 4.19; 4.29).…”

“…According to an estimate, there are about 1.5 to 3.5 million species of fungi in the world but this number is constantly increasing because of the never-ending search for these organisms throughout the world [1]. These organisms are known to about different soil types which are regarded as a reservoir for human infection [2].…”

“…Many researchers have demonstrated the presence of the Keratinophilic fungi from soils of playgrounds around the world. Most recent among the researchers include; Deshmukh et al [9], Shrivastava et al [12], Deshmukh and Verekan [13], Min Jung Lee et al [14], Rizwana et al [15], Gugnani et al [16], Maruthi et al [17], Agu et al [18], Pakshir et al [2], and Olajubu and Folorunso [19].…”

Notas micológicas XI:Tritirachium oryzae, Paecilomyces formosus y alcances en la Sección Fumigati de Aspergillus con énfasis en el complejo Aspergillus viridinutans

“…Es un género relacionado con Beauveria, Engyodontium y Aphanocladium ( De Hoog 1972-1973 Gams et al, 1984). Sus especies parecen ser saprotrofas, en ambientes diversos así como patógenas de insectos (Pakshir, et al, 2013). Incluso se ha detectado una especie en ambientes marinos (T.album) ( Wiese et al2011), mientras T. oryzae, se presenta en suelo, aire, vegetación y ambientes internos (Samson 2009), no existe información relacionada a la inhalación de sus conidios y los posibles efectos tóxicos o alergénicos.…”