Isolation and molecular detection of Pasteurella multocida Type A from naturally infected chickens, and their histopathological evaluation in artificially infected chickens in Bangladesh

Panna, Sayedun Nahar; Nazir, K. H. M. Nazmul Hussain; Rahman, Bahanur; Ahamed, Sultan; Saroare, Md. Golam; Chakma, Shovon; Kamal, Tazrin; Majumder, Ummay Habiba

doi:10.5455/javar.2015.b104

Cited by 18 publications

(22 citation statements)

References 21 publications

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“…Liver was swollen and sometimes congested and multiple necrotic foci were observed throughout the dorsal surface. These findings were agreed with the reports of Panna et al (2015). Heart was enlarged and edematous, and trachea and lungs were hemorrhagic (Zahoor and Siddique, 2006).…”

Section: Postmortem Lesionssupporting

confidence: 92%

Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

Akter

Hossain

Hassan

et al. 2021

Res. Agric. Livest. Fish.

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An experiment was conducted to investigate the immune response induced in chickens by capsular extract of Pasteurella multocida isolated from rats wandering in and around the poultry farms. The rat isolate of P. multocida was isolated and identified by cultural, morphological, and biochemical characteristics, followed by capsular extract preparation and experimental vaccine development. The isolated P. multocida was found Gram-negative, non-motile, non-spore forming rod occurring singly or pains and occasionally as chains or filaments in Gram’s-staining method. The isolates consistently produced acid from dextrose, sucrose and mannitol but not fermented maltose or lactose. The Capsular antigen was extracted and confirmed by acriflavine test. Finally, experimental fowl cholera vaccine was prepared. Primary vaccination was performed at the dose rate of 5.6×107 CFU/ml through intramuscular and subcutaneous routes in birds of group A (10 birds) and group B (10 birds) and group C (10 birds) were control birds. Secondary vaccination was similarly performed after 15 days of primary vaccination in groups A and B. The levels of pre-vaccination and post-vaccination sera were determined by passive haemagglutination test. The passive haemagglutination antibody titre was recorded on 15 and 35 days of post vaccination in groups A and B. It was demonstrated that experimental capsular extract fowl cholera vaccine conferred 100% protection (p<0.01) against challenge infection and found to be safe. It could be suggested that after thorough field trial, the experimentally prepared capsular extract FC vaccine using rat isolate of P. multocida may be used side by side with conventional FC vaccine. Res. Agric., Livest. Fish.8(1): 117-124, April 2021

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Section: Postmortem Lesionssupporting

confidence: 92%

Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

Akter

Hossain

Hassan

et al. 2021

Res. Agric. Livest. Fish.

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“…Laboratory stock of pathogenic bacteria of poultry such as P. multocida, S. pullorum, S. gallinarum, and E. coli were revived by culturing onto Blood agar ( P. multocida ), Salmonella Shigella (SS) agar ( S. pullorum and S. gallinarum ) and Eosine Methylene Blue (EMB) agar ( E. coli ). P. multocida, S. pullorum, S. gallinarum, and E. coli were previously isolated from poultry and identified by cultural characteristics; biochemical tests and PCR assays (Matin et al., 2017; Panna et al., 2015; Parvej et al., 2016).…”

Section: Methodsmentioning

confidence: 99%

Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry

Ali

Islam

Hossen

et al. 2021

Veterinary Medicine & Sci

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The aim of the study was to determine the efficacy of neem leaf extract against multidrug resistant (MDR) pathogenic bacteria. Laboratory stock culture of Pasteurella multocida, Salmonella pullorum, Salmonella gallinarum and Escherichia coli was revived. Antibiogram profiles of these bacteria were determined by disc diffusion method. Ethanolic extract of neem leaf was prepared. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of neem leaf extract (112.5, 100, 50, 25, 12.5, 6.25 and 3.12 mg/ml) against MDR pathogenic bacteria of poultry were determined by double dilution method. The MIC and MBC of the neem leaf extract were 12.5 and 25 mg/ml, respectively for P. multocida, 50 and 100 mg/ml for S. pullorum and S. gallinarum, 100 and 112.5 mg/ml for E. coli. Neem leaf extracts exhibited bactericidal effect against MDR pathogenic bacteria of poultry.

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“…Incubation was carried out overnight at 37℃. The growing colonies were observed macroscopically and microscopically, followed by the catalase test (Panna et al 2015), oxidase test (Grehn and Müller 1989), indole test (Hunt et al 2001), and molecular con rmation through Polymerase Chain Reaction (PCR) assay.…”

Section: Methodsmentioning

confidence: 99%

Screening and Purification of NanB Sialidase from Pasteurella Multocida with Activity in Hydrolyzing Sialic Acid Neu5Acα(2-6)Gal

Nugroho

Kurnia

Tarigan

et al. 2021

Preprint

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Study on sialidases as antiviral agents has been widely performed, but many types of sialidase had not been tested for their antiviral activity. One of such sialidase is the NanB sialidase of Pasteurella multocida, which has never been isolated for further study. In this study, the activity of NanB sialidase was investigated in silico by docking the NanB sialidase of Pasteurella multocida to the Neu5Acα(2-6)Gal ligand. Additionally, some local isolates of Pasteurella multocida, which had the NanB gene were screened, and the proteins were isolated for further testing regarding their activity in hydrolyzing Neu5Acα(2-6)Gal. In silico studies showed that the NanB sialidase possesses an exceptional affinity towards forming a protein-ligand complex with Neu5Acα(2-6)Gal. This was further confirmed by showing that a dose of 0.258 U/ml (100%) NanB sialidase of Pasteurella multocida B018 can hydrolyze up to 44.28% of Neu5Acα(2-6)Gal in chicken red blood cells and 81.95% in rabbit red blood cells. This study suggested that the NanB sialidase of Pasteurella multocida B018 has a potent antiviral activity that can inhibit avian influenza virus infection.

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Isolation and molecular detection of Pasteurella multocida Type A from naturally infected chickens, and their histopathological evaluation in artificially infected chickens in Bangladesh

Cited by 18 publications

References 21 publications

Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry

Screening and Purification of NanB Sialidase from Pasteurella Multocida with Activity in Hydrolyzing Sialic Acid Neu5Acα(2-6)Gal

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