Isolation and Molecular Detection of E.coli from Common Respiratory Infections of Poultry in A.P.

Abstract

In the present work a total of 228 pooled nasal swabs, 228 pooled tracheal swabs samples from the ailing birds and 152 pooled tracheal tissues, 152 pooled lung tissues from the dead birds were collected from the 19 suspected poultry farms in A.P. and were labelled farm wise and specimen wise. The DNA was extracted from the samples by using Trizol method. The PCR and SYBR Green real time PCR tests was standardized by targeting 16 s rRNA gene. The study found that 12 farms were found to be positive for E.coli, with percent positivity of 63.15% by PCR and 71.2% by SYBR Green Real Time PCR. Among the 12 positive farms, 92(40.35% nasal swabs),106(51.75% tracheal swabs),64 (42.10% tracheal tissues) and 72 (47.36% lung tissues) were positive for E.coli by PCR and 118(49.56% nasal swabs),128(56.14% tracheal swabs),68(44.73% tracheal tissues) and 77(50.65% lung tissues) were positive for E.coli by SYBR Green Real Time PCR, nasal swabs and tracheal swabs were positive with positivity of 40.35 and 51.75% respectively by PCR , 106 and 128, nasal swabs and tracheal swabs were positive with positivity 46.49 and 56.14% by SYBR Green Real Time PCR. This study found that the confirmatory diagnosis of respiratory infections in poultry is accurate when histopathology, isolation and molecular detection methods like PCR and SYBR Green Real time PCR are used.

Multiplex PCR for ompT and iss genes of Escherichia coli isolated from chronic respiratory disease (CRD) broiler farms

“…In the existing study, the PCR method for detection showed an isolated rate reached 28% E. coli, lower than 42.1% and 47.3% recorded by Thopireddy (2023) from the trachea and lung tissue, respectively by PCR. In the present study, the higher percentage recorded in the conventional method rather than the molecular method related to the high sensitivity and specificity of PCR test for diagnosis of E. coli in clinical specimens (Ozbey and Muz, 2006;Tonu et al, 2011;Innis et al, 2012).…”