Isolation and Molecular Charcteristic of a Recombinant Feline Calicivirus from Qingdao, China

Abstract

Feline calicivirus (FCV) is a highly contagious pathogen that can cause seriously upper respiratory tract and oral disease in feline. Despite widespread vaccination, the prevalence of FCV remains high. In this study, the FCV qd/2019/china was isolated from domestic feline oropharyngeal swab which was collected in qingdao, China. The virus was purified with plaque assay and identified with PCR and IFA method. the capsid amino acid ,VP1, of qd/2019/china [1] showed sequence identity with other isolate ranging from 83.9% (ym3/2001/jp) to 91.1% (CH-JL4) .Sequence analysis of the capsid amino acid revealed that qd/2019/china was closely related to CH-JL4 and clustered with CH-JL4 in the phylogenetic tree. Phylogenetic analysis indicated that complete genome of qd/2019/china and CH-JL4 was also classified into the same cluster. While the recombination analysis with Simplot indicate that the qd/2019/china originated from the recombination of CH-JL4 and HRB-SS, and the region 3821–5301 nt originated from HRB-SS. Further, the region 3821–5301 nt belong to protease -polymerase (PP) of HRB-SS. Here we isolate a new FCV qd/2019/china, which may be a recombinant virus, These results were beneficial for understanding the evolution of FCV.

Analysis of port pollutant emission characteristics in United States based on multiscale geographically weighted regression

“…Based on the available studies, the compilation methods of ship discharge inventories in the world mainly include "top-down" method (fuel method and trade method) and "bottom-up" method (statistical method and dynamic method) (Liu et al, 2018). Liu (2020) used the trade data of Qingdao Port between 2005 and 2017 to establish the ship emission inventory through the trade method, mainly including five atmospheric pollutants: NOx, SO 2 , CO, PM 10 , and VOCs. Lee et al (2021) chose the "bottom-up" approach to create a comprehensive local discharge inventory of ships from the Incheon Port in 2019.…”