Isolation and molecular characterization of <i>Frankia</i> strains resistant to some heavy metals

Abdel-lateif, Khalid S.; Mansour, Samira R.; El-Badawy, Mohamed F.; Shohayeb, Mohamed

doi:10.1002/jobm.201800122

Cited by 10 publications

(3 citation statements)

References 41 publications

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“…A genome mining study identified that many nodule forming species of Frankia possess characteristics for metal homeostasis containing factors of metal transporters (like CopA and CopCD), the sulfur importer (CysPUWA), arsenite exporter (AqpS), and so on along with proteins for binding and modification in diverse and unique combinations [178]. Eleven distinct Frankia strains isolated from Casuarina roots confirmed resistance to heavy metals such as Cu, Co, and Zn [179]. Elucidation of the mechanisms of Pb resistance in the Frankia sp.…”

Section: Abiotic Stress Anthropogenic Influences and Actinorhizal Sym...mentioning

confidence: 99%

Paradigms of actinorhizal symbiosis under the regime of global climatic changes: New insights and perspectives

Chetri¹,

Rahman

Thomas

et al. 2022

J Basic Microbiol

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Nitrogen occurs as inert and inaccessible dinitrogen gaseous form (N2) in the atmosphere. Biological nitrogen fixation is a chief process that makes this dinitrogen (N2) accessible and bioavailable in the form of ammonium (NH4+) ions. The key organisms to fix nitrogen are certain prokaryotes, called diazotrophs either in the free‐living form or establishing significant mutual relationships with a variety of plants. On such examples is ~95–100 MY old incomparable symbiosis between dicotyledonous trees and a unique actinobacterial diazotroph in diverse ecosystems. In this association, the root of the certain dicotyledonous tree (~25 genera and 225 species) belonging to three different taxonomic orders, Fagales, Cucurbitales, and Rosales (FaCuRo) known as actinorhizal trees can host a diazotroph, Frankia of order Frankiales. Frankia is gram‐positive, branched, filamentous, sporulating, and free‐living soil actinobacterium. It resides in the specialized, multilobed, and coralloid organs (lateral roots but without caps), the root nodules of actinorhizal tress. This review aims to provide systematic information on the distribution and the phylogenetic diversity of hosts from FaCuRo and their micro‐endosymbionts (Frankia spp.), colonization mechanisms, and signaling pathways. We also aim to provide details on developmental and physiological imperatives for gene regulation and functional genomics of symbiosis, phenomenal restoration ecology, influences of contemporary global climatic changes, and anthropogenic impacts on plant–Frankia interactions for the functioning of ecosystems and the biosphere.

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Section: Abiotic Stress Anthropogenic Influences and Actinorhizal Sym...mentioning

confidence: 99%

Paradigms of actinorhizal symbiosis under the regime of global climatic changes: New insights and perspectives

Chetri¹,

Rahman

Thomas

et al. 2022

J Basic Microbiol

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“…Nakamurellaceae belongs to the suborder Frankineae , which consists of Gram-positive bacteria that help in nitrogen fixation. They are symbiotic prokaryotes with mild resistance to heavy metals (El dein Abdel-lateif et al 2018 ). Japanese microbiologist Kazonuri Nakamura changed the illegitimate genus name to the logical and validly published name Nakamurella in 2004 (Tao et al 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Complete genome of Nakamurella sp. PAMC28650: genomic insights into its environmental adaptation and biotechnological potential

Paudel

Ghimire

Han

et al. 2022

Funct Integr Genomics

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The mechanisms underlying the survival of bacteria in low temperature and high radiation are not yet fully understood. Nakamurella sp. PAMC28650 was isolated from a glacier of Rwenzori Mountain, Uganda, which species belonged to Nakamurella genus based on 16S rRNA phylogeny, ANI (average nucleotide identity), and BLAST Ring Image Generator (BRIG) analysis among Frankineae suborder. We conducted the whole genome sequencing and comparative genomics of Nakamurella sp. PAMC28650, to understand the genomic features pertaining to survival in cold environment, along with high UV (ultraviolet) radiation. This study highlights the role of polysaccharide in cold adaptation, mining of the UV protection-related secondary metabolites and other related to cold adaptation mechanism through different bioinformatics tools, and providing a brief overview of the genes present in DNA repair systems. Nakamurella sp. PAMC28650 contained glycogen and cellulose metabolism pathways, mycosporine-like amino acids and isorenieratene-synthesizing gene cluster, and a number of DNA repair systems. Also, the genome analysis showed osmoregulation-related genes and cold shock proteins. We infer these genomic features are linked to bacterial survival in cold and UV radiation.

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“…As Frankia spp. are apparently resistant to high concentrations of heavy metals such as Cu, Co, and Zn, ,− we hypothesized that copper tolerance in Frankia sp. strains involves Cu(II) complexation by organic ligands released to prevent Cu(II) from entering the cells.…”

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confidence: 99%

Frankobactin Metallophores Produced by Nitrogen-Fixing Frankia Actinobacteria Function in Toxic Metal Sequestration

et al. 2021

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A series of new metallophores, referred to as frankobactins, were extracted from cultures of the symbiotic and nitrogen-fixing actinobacterium Frankia sp. CH37. Structure elucidation revealed a 2-hydroxyphenyl-substituted oxazoline core and a chain composed of five proteinogenic and nonproteinogenic amino acids, suggesting nonribosomal peptide synthesis as the biosynthetic origin. By whole-genome sequencing, bioinformatic analysis, and comparison with other Frankia strains, the genetic locus responsible for the biosynthesis was detected. Spectrophotometric titration of frankobactin with Fe(III) and Cu(II) and mass spectrometry established the 1:1 (metal:frankobactin) coordination. Uptake experiments suggested that frankobactin A 1 (1) did not serve to recruit iron, but to detoxify Cu(II). As frankobactin A 1 prevents the cellular entry of Cu(II), it could play a crucial role in the symbiosis of Frankia sp. and its host in the reclamation of copper-contaminated soil.

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Isolation and molecular characterization of Frankia strains resistant to some heavy metals

Cited by 10 publications

References 41 publications

Paradigms of actinorhizal symbiosis under the regime of global climatic changes: New insights and perspectives

Paradigms of actinorhizal symbiosis under the regime of global climatic changes: New insights and perspectives

Complete genome of Nakamurella sp. PAMC28650: genomic insights into its environmental adaptation and biotechnological potential

Frankobactin Metallophores Produced by Nitrogen-Fixing Frankia Actinobacteria Function in Toxic Metal Sequestration

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