Isolation and molecular characterization of a relapsing fever Borrelia recovered from Ornithodoros rudis in Brazil

Muñoz-Léal, Sebastián; Faccini-Martínez, Álvaro A.; Costa, Francisco Borges; Marcili, Arlei; Mesquita, Eric T.K.C.; Marques, Edwaldo P.; Labruna, Marcelo B.

doi:10.1016/j.ttbdis.2018.03.008

Cited by 54 publications

(46 citation statements)

References 37 publications

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“…Primers, thermal conditions and PCR protocols were implemented following references in Table 1. While Borrelia venezuelensis RMA01 (Muñoz‐Leal et al., 2018) was used as positive control for 16S rRNA, flaB , glpQ and p66 genes, PCRs targeting ospC , clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA genes were performed without positive control. Amplicons of expected size were purified with Illustra ExoproStar (GE, Healthcare) and sequenced in an ABI 3500 instrument (Applied Biosystems/Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

Novel Borrelia genotypes in bats from the Macaregua Cave, Colombia

Muñoz-Léal

Faccini-Martínez

Pérez‐Torres

et al. 2020

Zoonoses and Public Health

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Bats have been implicated as reservoirs of relapsing fever group spirochaetes since the beginning of the last century. Recently, bat‐associated spirochaetes have been reported as human pathogens. In 1968, a spirochaete was detected in blood of the bat Natalus tumidirostris captured inside the Macaregua cave, Colombia. Data on this microorganism were never published again. The aim of this study was to evaluate the presence of Borrelia DNA in blood from bats of Macaregua cave. We performed molecular analyses using a genus‐specific real‐time PCR targeting the 16S rRNA to detect DNA of Borrelia in blood samples from 46 bats captured in the Macaregua cave. Positive samples were submitted to a battery of PCRs aiming to amply Borrelia 16S rRNA, flaB, glpQ, p66, ospC, clpA, clpX, nifS, pepX, pyrG, recG, rplB and uvrA genes. Seventeen samples were positive for Borrelia after real‐time PCR. With the exception of flaB gene, attempts to amplify further loci were unsuccessful. Nucleotide and amino acid divergences of four flaB haplotypes characterized from blood of Carollia perspicillata showed Borrelia burgdorferi sensu lato (Bbsl) as the most closely related group. A phylogenetic tree including 74 sequences of the genus confirmed this trend, since Borrelia genotypes detected in bats from Macaregua formed a monophyletic group basally positioned to Bbsl. Our results suggest that Borrelia genotypes characterized from bats roosting in the Macaregua cave might constitute a new taxon within the genus. This is the first molecular characterization of a Borrelia sp. in Colombia.

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Section: Methodsmentioning

confidence: 99%

Novel Borrelia genotypes in bats from the Macaregua Cave, Colombia

Muñoz-Léal

Faccini-Martínez

Pérez‐Torres

et al. 2020

Zoonoses and Public Health

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“…Sokoloski et al (2018) have suggested that the tick species Rhipicephalus microplus, Rhipicephalus sanguineus, and, primarily, Amblyomma sculptum are involved in the transmission of this agent in South America, however, no study has substantiated this hypothesis. In Southern Hemisphere there are only culture isolation of borreliae from the Borrelia recurrentis group (Muñoz-Leal et al, 2018), and the Borrelia theileri isolate from R. microplus (Cordeiro et al, 2018) as the only one found in ticks of domestic animals may suggest cross-reaction in serological data domestic animals (Cordeiro et al, 2012) and humans (Yoshinari et al, 2003). Four Borrelia spp.…”

Section: Resultsmentioning

confidence: 99%

First serological detection of Borrelia spp. in dogs in western Cuba

Cordeiro

Silva

Navarrete

et al. 2020

Rev. Bras. Parasitol. Vet.

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This study aimed to verify the presence of IgG antibodies against Borrelia burgdorferi sensu lato (s.l) in domestic dogs in western Cuba. Serum samples were analyzed by indirect enzyme-linked immunosorbent assay (ELISA), using crude antigens of a B. burgdorferi strain of North American origin. To verify the presence of Borrelia spp., deoxyribonucleic acid (DNA) extracted from individual blood samples was analyzed by nested-PCR, with markers targeted for amplification of portions of the flagellin B gene (flaB) present in Borrelia spirochetes. Ticks were also collected through inspection of the animals. Sera from 93 of 176 (52.84%) dogs were reactive to the indirect ELISA. Geographic prevalence varied from 54.35% (25/46) in Boyeros, 44.44% (20/45) in Cotorro, 66.67% (22/33) in Habana del Este, and 50% (26/52) in San José de las Lajas. There was no statistical difference between these tested variables. No blood samples analyzed were positive for the Borrelia flaB gene.

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“…There are ≈200 tick species described in Latin America (GUGLIELMONE et al, 2003;NAVA et al, 2017), including 73 species specifically in Brazil (GIANIZELLA et al, 2018;MUÑOZ-LEAL et al, 2018). Several species have been reported as vectors of bacteria, especially those of the genera Rickettsia and Borrelia (PADDOCK et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Molecular survey of flaviviruses and orthobunyaviruses in Amblyomma spp. ticks collected in Minas Gerais, Brazil

Binder

Tauro

Farías

et al. 2019

Rev. Bras. Parasitol. Vet.

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Due to anthropic environmental changes, vector-borne diseases are emerging worldwide. Ticks are known vectors of several pathogens of concern among humans and animals. In recent decades, several examples of tick-borne emerging viral diseases have been reported (Crimean Congo hemorrhagic fever virus, Powassan virus, encephalitis virus, heartland virus, severe fever with thrombocytopenia syndrome virus). Unfortunately, few studies addressing the presence of viruses in wild ticks have been carried out in South America. With the aim of detecting flaviviruses and orthobunyaviruses in ticks, we carried out molecular detection in wild ticks collected in the state of Minas Gerais, Brazil. No Flavivirus-positive ticks were detected; however, we detected activity of Orthobunyavirus in 8 Amblyomma tick specimens. One of those individuals was positive for Bunyamwera orthobunyavirus, which represents the first report of this virus among ticks in South America. Further studies related to the ecology of zoonotic diseases are needed to increase knowledge of this topic, including attempts at viral isolation, full genome sequencing and biological characterization. In this way, we will obtain a better picture of the real risk of ticks as a vector for viral diseases for humans and animals on our continent, where no tick-borne viral disease is known to occur.

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Isolation and molecular characterization of a relapsing fever Borrelia recovered from Ornithodoros rudis in Brazil

Cited by 54 publications

References 37 publications

Novel Borrelia genotypes in bats from the Macaregua Cave, Colombia

Novel Borrelia genotypes in bats from the Macaregua Cave, Colombia

First serological detection of Borrelia spp. in dogs in western Cuba

Molecular survey of flaviviruses and orthobunyaviruses in Amblyomma spp. ticks collected in Minas Gerais, Brazil

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