Isolation and molecular characterization of a novel strain of Bacillus with antifungal activity from the sorghum rhizosphere

Martínez-Absalón, Sofía; Orozco-Mosqueda, Ma. del Carmen; Martínez-Pacheco, Mauro M.; Farías‐Rodríguez, Rodolfo; Govindappa, M.; Santoyo, Gustavo

doi:10.4238/2012.july.10.15

Cited by 17 publications

(15 citation statements)

References 23 publications

(30 reference statements)

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“…As highest identity match found in databases of Bacillus species was 91% identity so this suggested that Bacillus sp. UM96 might be a novel species 19 .…”

Section: Antibiotics Produced By Bacteriamentioning

confidence: 99%

Untitled

2016

IJPSR

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ABSTRACT:The rhizosphere represents the thin layer of soil surrounding plant roots and the soil occupied by the roots, supports large active groups of microorganisms. The vast organic compounds (amino acids, sugars etc.) secreted by plant roots in the rhizosphere provide a food source for microorganisms increasing microbial biomass and their activity in the rhizosphere. Antibiotics are antimicrobial compounds produced by living microorganism as secondary metabolites. These compounds are used therapeutically and sometimes prophylactically in the control of infectious diseases. The isolation of antibiotics from microorganisms is relatively easy as compared to chemical synthesis of antimicrobial agents. The isolation of antibiotics from microorganisms improved the discovery of novel antibiotics that could act as better chemotherapeutic agents. With the increased population pressure, costs and side effects and the development of resistance of pathogens to drugs for infectious diseases, there is an urgent need to explore microbes for development of new antimicrobial metabolites. As microorganisms grow in unique and extreme habitats, they may have the capability to produce unique and unusual metabolites. So rhizospheric soil gives an excellent option as a source for search of some new alternative medicines. This review highlights the recent developments in the production of antimicrobial compounds from rhizospheric soil microflora.

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“…As highest identity match found in databases of Bacillus species was 91% identity so this suggested that Bacillus sp. UM96 might be a novel species 19 .…”

Section: Antibiotics Produced By Bacteriamentioning

confidence: 99%

Untitled

2016

IJPSR

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“…Recently (Martínez-Absalón et al, 2012), a strain of Bacillus sp (UM96) was isolated, and its antifungal and hemolytic activities were characterized. Such strains showed strong inhibition against the same fungal phytopathogens analyzed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Other kinds of hemolysis, including alpha and gamma, were also observed. However, previous analyses have shown that strains with these kinds of activities do not show strong antibiotic or fungitoxic action, and hence, these were excluded from further analysis (Martínez-Absalón et al, 2012).…”

Section: Hemolytic Analysismentioning

confidence: 99%

“…Molecular typing and phylogenetic analyses were carried out as previously described (Martínez-Absalón et al, 2012). Genomic DNA was isolated from 8 antagonistic isolates (UM15, UM24, UM33, UM42, UM49, UM52, UM88, and UM91) to amplify by PCR (Sambrook et al, 1982) the ribosomal 16S rRNA gene by using the universal bacterial primers Fd1, 5ꞌ-CAGAGTTTGATCCTGGCTCAG-3ꞌ and Rd1, 5ꞌ-AAGGAGGTGATCCAGCC-3ꞌ (Weisburg et al, 1991).…”

Section: Molecular Typing and Phylogenetic Analysismentioning

confidence: 99%

“…Species of this genus have both direct and indirect mechanisms to improve plant growth (Santoyo et al, 2012). PGPR of this genus are known to produce various antibiotics, volatile organic compounds (VOCs), bacteriocins, and lipopeptides (Martínez-Absalón et al, 2012;Velázquez-Becerra et al, 2013). In particular, Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus thuringiensis produce several kinds of lipopeptides such as surfactin, fengicin, iturin A, bacilomicin, and mycosubtilin, which have been shown to suppress plant pathogens (Tsuge et al, 2001;Yao et al, 2003;Koumoutsi et al, 2004;Ongena and Jacques, 2008;Martínez-Absalón et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants

Hernández-Salmerón¹,

Prieto-Barajas²,

Valencia-Cantero³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of Medicago truncatula plants. Of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10 -2 ). Hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic DNA, yielding 88 haplotypes. The similarity coefficient of Nei and Li showed a polymorphic diversity ranging from 0.3 to 1. Additionally, 8 of the hemolytic isolates showed antifungal activity toward plant pathogens, Diaporthe phaseolorum, Colletotrichum acutatum, Rhizoctonia solani, and Fusarium oxysporum. The 16S ribosomal sequencing analysis showed that antagonistic bacterial isolates corresponded to Bacillus subtilis (UM15, UM33, UM42, UM49, UM52, and UM91), Bacillus pumilus (UM24), and Bacillus licheniformis (UM88). The present results revealed a higher genetic diversity among hemolytic isolates compared to that of isolates with antifungal action.

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Bacillus thuringiensis as a biofertilizer in crops and their implications in the control of phytopathogens and insect pests

Gomis-Cebolla

Berry

2023

Pest Management Science

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BACKGROUND Bacillus thuringiensis (Bt) is a spore‐forming bacterium that produces insecticidal proteins and other virulence factors and is considered one of the most successful bioinsecticides available to control pests in agriculture. Currently, some Bt strains have been reported as endophyte or rhizospheric bacteria. RESULTS Little is known about the implications of plant‐Bt interaction in crop protection. Here, we review if Bt can establish as an endophyte/rhizobacterium and evaluate if Bt as an endophyte/rhizobacterium can simultaneously act against different phytopathogens (fungi, bacteria, insects and viruses) plus promote plant growth. CONCLUSION Although Bt produce an arsenal of proteins with toxic effects against insect, the current knowledge suggests that Bt can be considered as a promising new plant growth promotion bacterium (PGPB). The implications of the proposed review will broaden our understanding of Bt as a versatile entomopathogen that may be able to exhibit differential behavior depending on context. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Isolation and molecular characterization of a novel strain of Bacillus with antifungal activity from the sorghum rhizosphere

Cited by 17 publications

References 23 publications

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Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants

Bacillus thuringiensis as a biofertilizer in crops and their implications in the control of phytopathogens and insect pests

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