Isolation and molecular characterization of Salmonella enterica serovar Javiana from food, environmental and clinical samples

Mezal, Ezat H.; Stefanova, Rossina; Khan, Ashraf A.

doi:10.1016/j.ijfoodmicro.2013.03.021

Cited by 44 publications

(47 citation statements)

References 22 publications

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“…It is also widespread in shelter dogs in Texas, United States (Leahy et al 2016). This serovar has a low infectious dose and possesses several virulence genes and plasmids that can contribute to large salmonellosis outbreaks in humans (Elward et al 2006;Mezal et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Serovars and antimicrobial resistance of non‐typhoidal Salmonella isolated from non‐diarrhoeic dogs in Grenada, West Indies

Amadi

Hariharan

Arya

et al. 2017

Veterinary Medicine & Sci

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Non‐typhoidal salmonellosis remains an important public health problem worldwide. Dogs may harbour Salmonella in their intestines and can easily shed Salmonella in the environment with the possibility of transmission to humans. Thus, monitoring is essential to understand the role of dogs in zoonotic transmission. The objectives of this study were to determine the shedding of Salmonella by owned, apparently healthy dogs in Grenada, West Indies, to identify the serovars, and to examine their antimicrobial susceptibility profiles. Faecal samples collected during August to October, 2016 from 144 non‐diarrhoeic owned dogs were examined by enrichment and selective culture for the presence of Salmonella spp. Eight (5.6%) of the tested animals were culture positive, yielding 35 Salmonella isolates that belonged to six serovars of Salmonella enterica subspecies enterica. These were serovars Arechavaleta from two dogs, Arechavaleta and Montevideo from one dog, and Javiana, Rubislaw, Braenderup and Kiambu from one dog each. All these serovars have been reported as causes of human salmonellosis globally. Antimicrobial susceptibility tests on 35 isolates showed absence of resistance to the currently used drugs for cases of human salmonellosis, including ciprofloxacin and cefotaxime. One isolate (2.9%) was resistant to neomycin, two isolates (5.7%) showed intermediate susceptibility to neomycin, and another (2.9%) had intermediate susceptibility to tetracycline. This is the first report of isolation and antimicrobial susceptibilities of non‐typhoidal Salmonella serovars from dogs in Grenada. This study shows that dogs in Grenada may be involved in the epidemiology of salmonellosis.

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Section: Discussionmentioning

confidence: 99%

Serovars and antimicrobial resistance of non‐typhoidal Salmonella isolated from non‐diarrhoeic dogs in Grenada, West Indies

Amadi

Hariharan

Arya

et al. 2017

Veterinary Medicine & Sci

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“…These findings confirm that the poultry house isolates are capable of contributing to human infection. Recently, Akiyama et al (2011) and Mezal et al (2013) indicated that the isolates from the environment carried the same virulence genes as clinical isolates, which are capable of causing human infections.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmid profile analysis has been helpful for the characterization of many Salmonella serovars including ser. Enteritidis (Mezal et al, 2013;Ridley et al, 1998). Many strains of Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular characterization of Salmonella enterica serovar Enteritidis from poultry house and clinical samples during 2010

et al. 2014

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b s t r a c tA total of 60 Salmonella enterica serovar (ser.) Enteritidis isolates, 28 from poultry houses and 32 from clinical samples, were isolated during 2010. These isolates were subjected to testing and analyzed for antibiotic resistance, virulence genes, plasmids and plasmid replicon types. To assess genetic diversity, pulsed-field gel electrophoresis (PFGE) fingerprinting, using the XbaI restriction enzyme, Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) and plasmid profiles were performed. All isolates from poultry, and 10 out of 32 clinical isolates were sensitive to ampicillin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, sulfisoxazole, streptomycin, and tetracycline. Twenty-one of thirty-two clinical isolates were resistant to ampicillin and tetracycline, and one isolate was resistant to nalidixic acid. PFGE typing of sixty ser. Enteritidis isolates by XbaI resulted in 10e12 bands and grouped into six clusters each with similarity from 95% to 81%. The MLVA analysis of sixty isolates gave 18 allele profiles with the majority of isolates displayed in three groups, and two clinical isolates found to be new in the PulseNet national MLVA database. All isolates were positive for 12 or more of the 17 virulence genes mostly found in S. enterica (spvB, spiA, pagC, msgA, invA, sipB, prgH, spaN, orgA, tolC, iroN, sitC, IpfC, sifA, sopB, and pefA) and negative for one gene (cdtB). All isolates carried a typical 58 kb plasmid, type Inc/FIIA. Three poultry isolates and one clinical isolate carried small plasmids with 3.8, 6, 7.6 and 11.5 kb. Ten of the clinical isolates carried plasmids, with sizes 36 and 38 kb, types IncL/M and IncN, and one isolate carried an 81 kb plasmid, type IncI. Southern hybridization of a plasmid with an Inc/FIIA gene probe hybridized one large 58 kb plasmid in all isolates. Several large and small plasmids from poultry isolates were not typed by our PCR-based method. These results confirmed that PFGE fingerprinting has limited discriminatory power for ser. Enteritidis in both poultry and clinical sources. However, the plasmid and MLVA allele profiles were a useful and important epidemiology tool to discriminate outbreak strains of ser. Enteritidis from poultry and clinical samples.Published by Elsevier Ltd.

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“…The cdt is defined as a bacterial genotoxin and has been reported to be present in several food-and water-associated pathogens, including several pathotypes of Escherichia coli, and species of Campylobacter and Shigella ( Jinadasa et al, 2011). Recently the presence of cdtB has been reported in some NTS serovars, including Schwarzengrund, Montevideo, Bredeney, 9,12:l,v:-, and Javiana (den Bakker et al, 2011;Mezal et al, 2013;Suez et al, 2013). The genome sequence of S. Javiana (CFSAN001992) has been closed and showed the presence of the cdtB (Allard et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Mechanism ofCytolethal Distending Toxinin NontyphoidalSalmonellaSerovar (SalmonellaJaviana) During Macrophage Infection

Williams

Gokulan

Shelman

et al. 2015

DNA and Cell Biology

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Cytolethal distending toxin B (cdtB) is a conserved virulence factor in Salmonella enterica serovar Typhi. Here we report the presence and functionality of cdtB in some nontyphoidal Salmonella (NTS) serovars, including Salmonella Javiana (cdtB+wt S. Javiana), isolated from imported food. To understand the role of cdtB in NTS serovars, a deletion mutant (cdtB(-)ΔS. Javiana) was constructed. Macrophages were infected with cdtB+wt S. Javiana (wild type), cdtB(-)Δ S. Javiana (mutant), and cdtB-negative NTS serovar (S. Typhimurium). Cytotoxic activity and transcription level of genes involved in cell death (apoptosis, autophagy, and necrosis) were assessed in infected macrophages. The cdtB+wt S. Javiana caused cellular distension as well as high degree of vacuolization and presence of the autophagosome marker LC3 in infected macrophages as compared with cdtB(-)ΔS. Javiana. The mRNA expression of genes involved in the induction of autophagy in response to toxin (Esr1 and Pik3C3) and coregulators of autophagy and apoptosis (Bax and Cyld) were significantly upregulated in cdtB(+)wt S. Javiana-infected macrophages. As autophagy destroys internalized pathogens in addition to the infected cell, it may reduce the spread of infection.

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Isolation and molecular characterization of Salmonella enterica serovar Javiana from food, environmental and clinical samples

Cited by 44 publications

References 22 publications

Serovars and antimicrobial resistance of non‐typhoidal Salmonella isolated from non‐diarrhoeic dogs in Grenada, West Indies

Serovars and antimicrobial resistance of non‐typhoidal Salmonella isolated from non‐diarrhoeic dogs in Grenada, West Indies

Isolation and molecular characterization of Salmonella enterica serovar Enteritidis from poultry house and clinical samples during 2010

Cytotoxic Mechanism ofCytolethal Distending Toxinin NontyphoidalSalmonellaSerovar (SalmonellaJaviana) During Macrophage Infection

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