Isolation and Molecular Characterization of Arsenite-Tolerant<i>Alishewanella</i>sp. GIDC-5 Originated from Industrial Effluents

Jain, Raina; Jha, Sanjay Kumar; Adhikary, Hemanta; Kumar, Prasant; Parekh, Vipulkumar; Jha, Anamika; Mahatma, M. K.; Archana, G.

doi:10.1080/01490451.2013.811317

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…SG13. NCBI#:HQ413096), a human fetus [55] , tidal sediments [56] , fermented seafood [57] , landfills [58] , textile dye contaminated soils [59] , and industrial effluents [60] . According to a previous study by Kim et al 2010 [58] , Alishewanella agri BL06 can grow in the pH range between 5.5 and 12.0.…”

Section: Discussionmentioning

confidence: 99%

Application of Ion Torrent Sequencing to the Assessment of the Effect of Alkali Ballast Water Treatment on Microbial Community Diversity

et al. 2014

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The impact of NaOH as a ballast water treatment (BWT) on microbial community diversity was assessed using the 16S rRNA gene based Ion Torrent sequencing with its new 400 base chemistry. Ballast water samples from a Great Lakes ship were collected from the intake and discharge of both control and NaOH (pH 12) treated tanks and were analyzed in duplicates. One set of duplicates was treated with the membrane-impermeable DNA cross-linking reagent propidium mono-azide (PMA) prior to PCR amplification to differentiate between live and dead microorganisms. Ion Torrent sequencing generated nearly 580,000 reads for 31 bar-coded samples and revealed alterations of the microbial community structure in ballast water that had been treated with NaOH. Rarefaction analysis of the Ion Torrent sequencing data showed that BWT using NaOH significantly decreased microbial community diversity relative to control discharge (p<0.001). UniFrac distance based principal coordinate analysis (PCoA) plots and UPGMA tree analysis revealed that NaOH-treated ballast water microbial communities differed from both intake communities and control discharge communities. After NaOH treatment, bacteria from the genus Alishewanella became dominant in the NaOH-treated samples, accounting for <0.5% of the total reads in intake samples but more than 50% of the reads in the treated discharge samples. The only apparent difference in microbial community structure between PMA-processed and non-PMA samples occurred in intake water samples, which exhibited a significantly higher amount of PMA-sensitive cyanobacteria/chloroplast 16S rRNA than their corresponding non-PMA total DNA samples. The community assembly obtained using Ion Torrent sequencing was comparable to that obtained from a subset of samples that were also subjected to 454 pyrosequencing. This study showed the efficacy of alkali ballast water treatment in reducing ballast water microbial diversity and demonstrated the application of new Ion Torrent sequencing techniques to microbial community studies.

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Section: Discussionmentioning

confidence: 99%

Application of Ion Torrent Sequencing to the Assessment of the Effect of Alkali Ballast Water Treatment on Microbial Community Diversity

et al. 2014

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“…These results were mostly consistent with those of our previous studies in the strongly reducing area conducted with traditional sequencing and 454 pyrosequencing methods (Li et al, 2013, 2015). Predominant populations in high As groundwater samples such as Alishewanella and Psychrobacter were previously isolated from industrial eﬄuent or groundwater aquifers, showing the capability of hyper arsenite tolerance or arsenate reduction (Liao et al, 2011; Jain et al, 2014). In some recent studies, species of Alishewanella were also identified as denitrifiers (Kolekar et al, 2013; Liu et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Microbial Community of High Arsenic Groundwater in Agricultural Irrigation Area of Hetao Plain, Inner Mongolia

Wang

Jiang

et al. 2016

Front. Microbiol.

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Microbial communities can play important role in arsenic release in groundwater aquifers. To investigate the microbial communities in high arsenic groundwater aquifers in agricultural irrigation area, 17 groundwater samples with different arsenic concentrations were collected along the agricultural drainage channels of Hangjinhouqi County, Inner Mongolia and examined by illumina MiSeq sequencing approach targeting the V4 region of the 16S rRNA genes. Both principal component analysis and hierarchical clustering results indicated that these samples were divided into two groups (high and low arsenic groups) according to the variation of geochemical characteristics. Arsenic concentrations showed strongly positive correlations with NH4+ and total organic carbon (TOC). Sequencing results revealed that a total of 329–2823 operational taxonomic units (OTUs) were observed at the 97% OTU level. Microbial richness and diversity of high arsenic groundwater samples along the drainage channels were lower than those of low arsenic groundwater samples but higher than those of high arsenic groundwaters from strongly reducing areas. The microbial community structure in groundwater along the drainage channels was different from those in strongly reducing arsenic-rich aquifers of Hetao Plain and other high arsenic groundwater aquifers including Bangladesh, West Bengal, and Vietnam. Acinetobacter and Pseudomonas dominated with high percentages in both high and low arsenic groundwaters. Alishewanella, Psychrobacter, Methylotenera, and Crenothrix showed relatively high abundances in high arsenic groundwater, while Rheinheimera and the unidentified OP3 were predominant populations in low arsenic groundwater. Archaeal populations displayed a low occurrence and mainly dominated by methanogens such as Methanocorpusculum and Methanospirillum. Microbial community compositions were different between high and low arsenic groundwater samples based on the results of principal coordinate analysis and co-inertia analysis. Other geochemical variables including TOC, NH4+, oxidation-reduction potential, and Fe might also affect the microbial composition.

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“…Intensification of energy-related and other industrial processes associated with production of wastes and by-products rich in heavy metals and other xenobiotic compounds has led to serious soil contamination of many industrial sites. The resultant accumulations of the various organic chemicals in the environment, particularly in soil, are of significant concern because of their toxicity, including their carcinogenicity, and also because of their potential to bioaccumulate in living systems (Jain et al, 2014). Thus, the intracellular concentration of heavymetal ions has to be strongly controlled.…”

Section: )mentioning

confidence: 99%

Burkholderia cepacia BAM-12 isolated from mungbean field in Rajasthan augments plant growth in agricultural field soil of Gujarat, India

Jha

Popat²

2021

J microb biotech food sci

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Application of exotic bacterial strains as biofertilizer has always posed a constraint in the success of biofertilizer technology. Only a limited number of biofertilizers with applicability in a wide range of soil conditions is available in the market. Pseudomonas cepacia BAM-12 (MTCC No. 7100) (now known as Burkholderia cepacia), an isolate from the mungbean rhizosphere of the agricultural field of Rajasthan has shown remarkable plant growth promotion in Gujarat agricultural field soil. B. cepacia was found to increase the overall growth of 3 agriculturally important plants widely grown in Gujarat region, viz., mungbean, maize and rice. Increased plant growth was assessed on the basis of increased leaf number and area, biomass, chlorophyll content, profuse adventitious root branching, increased nodulation (in mungbean only), high available P in soil and overall plant growth in B. cepacia treated plants. Vital factor towards increased plant growth was the amount of IAA secreted by the organism (i.e. 2.65 mg IAA/100 ml of the culture filtrate) which is reasonably high in comparison to earlier reports available. Furthermore, PSB has also demonstrated bioremediation potential against harmful heavy metals such as copper, lead, nickel and arsenic by tolerating upto 25 mM indicating it’s prospective in remediating heavy metal contaminated agricultural fields.

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Isolation and Molecular Characterization of Arsenite-TolerantAlishewanellasp. GIDC-5 Originated from Industrial Effluents

Cited by 7 publications

References 37 publications

Application of Ion Torrent Sequencing to the Assessment of the Effect of Alkali Ballast Water Treatment on Microbial Community Diversity

Application of Ion Torrent Sequencing to the Assessment of the Effect of Alkali Ballast Water Treatment on Microbial Community Diversity

Microbial Community of High Arsenic Groundwater in Agricultural Irrigation Area of Hetao Plain, Inner Mongolia

Burkholderia cepacia BAM-12 isolated from mungbean field in Rajasthan augments plant growth in agricultural field soil of Gujarat, India

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