Isolation and Molecular Characterization of the Fire Blight Pathogen, Erwinia amylovora, Isolated from Apple and Pear Orchards in Egypt

Abstract

Eleven Erwinia amylovora isolates, the causal agent of the fire blight disease, were isolated from apple orchards adjacent to those of pears showing fire blight disease symptoms. Initial characterization concerning morphological and biochemical traits revealed that all isolates were typical to those of E. amylovora. Furthermore, all bacterial isolates showed typical symptoms of the fire blight disease upon infection of unripe pear fruits. Molecular characterization of these isolates via PCR utilizing specific primers based on a region of plasmid pEA29 and chromosomal DNA ams-region was performed. PCR products were positive and represented the expected length 1.1 and 1.6 kb, respectively. Moreover, PCR reactions utilizing the 16S rRNA universal primers were carried out. DNA sequence of PCR products and analysis via blast and Genbank data showed that, the bacterial isolates actually belonging to E. amylovora. Moreover, there are interference between these Erwinia isolates and identified E. amylovora strains based on constructed phylogenetic analysis. To the best of our knowledge this is the first report of fire blight of apples caused by E. amylovora in Egypt.

Isolation and characterization of Priestia megaterium KD7 for the biological control of pear fire blight

“…Genomic DNA was extracted using CTAB/NaCl method ( Ashmawy et al, 2015 ). The bacterial isolates were identified by PCR of the 16S rDNA gene sequence using fD2 (5′- AGAGTTTGA TCCTGGCTCAG-3′) and rP1 (5’-ACGGTTACCTTGT TACGACTT-3′) primers ( Weisburg et al, 1991 ).…”

Nano-Metals Forming Bacteria in Egypt. II. Efficacy towards Biomolecules, Ultrastructure, Growth Parameters, and Eco-Friendly Therapeutic of Soft Rot/Blackleg Genera

“…Ten most virulent and important bacterial plant pathogens based on their pathogenicity level, economic impact, and molecular aspects were listed1. Most devastating bacterial plant pathogens species were isolated in Egypt which belong to Pseudomonas syringae pathovars 3,4,5,6 , Ralstonia solanacearum 7,8,9 , Agrobacterium tumefaciens 9,10,11,12 , Xanthomonas 3,13,14 , Erwinia amylovora 15,16,17,18,19,20 and Xylella fastidiosa 21. Bacterial soft rot soft rots commonly affect vegetables and fruits. It can occur on crops in the eld and on the market.…”

Phylogenetic Analysis of Pathogenic Bacteria Belongs to the Erwinia Genus – Agent of Apple’s Diseases in Ukraine

“…Рід Erwinia − один із найстаріших родів бактерій, названий на честь видатного американського фітопатолога Ервіна Франка Сміта (1854-1927), що об'єднує асоційовані з рослиною епіфітні, сапрофітні і фітопатогенні види бактерії [3,6]. Зокрема типовим представником даного роду є карантинний для більшості країн світу, у тому числі України, вид Erwinia amylovora -збудник бактеріального опіку плодових, що здатен уражувати близько 170 видів рослин [1,3,6,9]. Всебічне дослідження біологічних властивостей представників даного роду повсякчас привертає увагу науковців усього світу.…”