Isolation and molecular analysis of six taproot expressed genes from sugar beet

Kloos, Dorothee U.; Oltmanns, Heiko; Dock, Christiane; Stahl, Dietmar J.; Hehl, Reinhard

doi:10.1093/jexbot/53.373.1533

Cited by 13 publications

(13 citation statements)

References 7 publications

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“…In some cases the relative expression values are more than 1,000 times above background levels. These results are consistent with the taproot expression of the Mll, Tlp, and His1-r genes analysed earlier by Northern blot experiments (Kloos et al 2002). When reporter gene expression by the three different promoters is compared, the Mll promoter shows for most of the lines no detectable leaf expression and taproot expression reaches the highest levels compared to Tlp, and His1-r. To further investigate the root specificity of the Mll gene, RNA blot hybridizations were carried out with RNA isolated from different sugar beet tissues of 4-19 week old plants.…”

Section: Preferential Reporter Gene Expression In the Sugar Beet Taprootsupporting

confidence: 90%

“…The cloning of the Tlp, Mll, and His1-r genes has been described earlier (Kloos et al 2002). For the isolation of genomic clones encoding these genes, a genomic sugar beet library was used .…”

Section: Methodsmentioning

confidence: 99%

“…Radioactive probes were generated by labelling 20 ng of DNA with 1.85 MBq P 32 -dATP (185 TBq/mMol, Amersham Pharmacia Biotech). The 802 bp cDNA of the Mll gene was used as a probe (Kloos et al 2002). Electrophoretic separation of RNA, transfer to Hybond nylon membranes (Amersham Pharmacia Biotech), hybridization to radioactive probes, and exposure of the membrane to X-ray films were done according to standard protocols .…”

Section: Rna Blot Hybridizationsmentioning

confidence: 99%

“…The modification of storage root specific traits requires promoters that are specifically expressed in storage root tissues. Three recently isolated taproot expressed genes are Mll, a homologue of the major latex-like protein (Mll) from Mesembryanthemum crystallinum, a thaumatin-like protein (Tlp), and a linker histone (His1-r) variant (Kloos et al 2002). To investigate their tissue specificity, the putative promoters were isolated, linked to reporter genes and stably transformed into sugar beet and tobacco.…”

Section: Introductionmentioning

confidence: 99%

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Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

Oltmanns¹,

Kloos²,

Brieß³

et al. 2006

Planta

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The storage root (taproot) of sugar beet (Beta vulgaris L.) originates from hypocotyl and primary root and contains many different tissues such as central xylem, primary and secondary cambium, secondary xylem and phloem, and parenchyma. It was the aim of this work to characterize the promoters of three taproot-expressed genes with respect to their tissue specificity. To investigate this, promoters for the genes Tlp, His1-r, and Mll were cloned from sugar beet, linked to reporter genes and transformed into sugar beet and tobacco. Reporter gene expression analysis in transgenic sugar beet plants revealed that all three promoters are active in the storage root. Expression in storage root tissues is either restricted to the vascular zone (Tlp, His1-r) or is observed in the whole organ (Mll). The Mll gene is highly organ specific throughout different developmental stages of the sugar beet. In tobacco, the Tlp and Mll promoters drive reporter gene expression preferentially in hypocotyl and roots. The properties of the Mll promoter may be advantageous for the modification of sucrose metabolism in storage roots.

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Section: Preferential Reporter Gene Expression In the Sugar Beet Taprootsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Rna Blot Hybridizationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

Oltmanns¹,

Kloos²,

Brieß³

et al. 2006

Planta

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“…Although the quality of the beet roots have been studied extensively during storage, little is known about post-harvest gene expression and the response to post-harvest stress conditions. Earlier gene expression studies in sugar beet concentrated on the taproot and on leaves (Bellin et al 2002;Kloos et al 2002;Stahl et al 2004;Oltmanns et al 2006;Bellin et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Post-harvest regulated gene expression and splicing efficiency in storage roots of sugar beet (Beta vulgaris L.)

Rotthues

Kappler

Lichtfuss

et al. 2008

Planta

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Sixteen post-harvest upregulated genes from sugar beet comprising five novel sequences were isolated by subtractive cloning. Transcription profiles covering a period of up to 49 days after harvest under controlled storage conditions and in field clamps are reported. Post-harvest induced genes are involved in wound response, pathogen defense, dehydration stress, and detoxification of reactive oxygen species. An early induction of a cationic peroxidase indicates a response to post-harvest damage. Wound response reactions may also involve genes required for cell division such as a regulator of chromatin condensation and a precursor of the growth stimulating peptide phytohormone phytosulfokine-alpha. Surprisingly, also three putative non-protein coding genes were isolated. Two of these genes show intron specific and storage temperature dependent splicing of a precursor mRNA. The temperature dependent splicing of an intron containing sugar beet mRNA is also maintained in transgenic Arabidopsis thaliana. The storage induced genes are integrated into a model that proposes the response to several post-harvest stress conditions. Temperature regulated splicing may be a mechanism to sense seasonal temperature changes.

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The cassava (Manihot esculenta Crantz) root proteome: Protein identification and differential expression

et al. 2006

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Using high-resolution 2-DE, we resolved proteins extracted from fibrous and tuberous root tissues of 3-month-old cassava plants. Gel image analysis revealed an average of 1467 electrophoretically resolved spots on the fibrous gels and 1595 spots on the tuberous gels in pH 3-10 range. Protein spots from both sets of gels were digested with trypsin. The digests were subjected to nanoelectrospray quadrupole TOF tandem mass analysis. Currently, we have obtained 299 protein identifications for 292 gel spots corresponding to 237 proteins. The proteins span various functional categories from energy, primary and secondary metabolism, disease and defense, destination and storage, transport, signal transduction, protein synthesis, cell structure, and transcription to cell growth and division. Gel image analysis has shown unique, as well as up- and down-regulated proteins, present in the tuberous and the fibrous tissues. Quantitative and qualitative analysis of the cassava root proteome is an important step towards further characterization of differentially expressed proteins and the elucidation of the mechanisms underlying the development and biological functions of the two types of roots.

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Isolation and molecular analysis of six taproot expressed genes from sugar beet

Cited by 13 publications

References 7 publications

Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

Post-harvest regulated gene expression and splicing efficiency in storage roots of sugar beet (Beta vulgaris L.)

The cassava (Manihot esculenta Crantz) root proteome: Protein identification and differential expression

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