Isolation and mapping of 88 new RFLP markers on human chromosome 8

Emi, Mitsuru; Takáhashi, Eiichi; Koyama, Kumiko; Okui, Keiko; Oshimura, Mitsuo; Nakamura, Yusuke

doi:10.1016/0888-7543(92)90044-s

Cited by 20 publications

(13 citation statements)

References 29 publications

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“…T h e locations of pCTSB and cEGR3-3 on the genetic map were determined by painvise linkage analysis against each of the abovementioned 14 markers. Markers cCI8-1, cCI8-586, cCI8-277, and cCI8-340 were incorporated into the map on the basis of their physical locations determined by fluorescence in situ hybridization (Emi et al, 1992b).…”

Section: Southern Blot Hybridizationmentioning

confidence: 99%

Allelic loss at chromosome band 8p21.3‐p22 is associated with progression of hepatocellular carcinoma

Emi

Fujiwara

Ohata

et al. 1993

Genes Chromosomes & Cancer

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Human hepatocellular carcinomas (HCC) frequently show loss of heterozygosity at loci on the short arm of chromosome 8. To define a region on 8p commonly deleted in HCC, we used 20 restriction fragment length polymorphism markers to carry out detailed deletion mapping in 142 HCC. Of the 124 informative cases, 56 (45%) showed allelic losses in tumors. While more than half of them had lost alleles at every locus on the short arm, others lost them partially or interstitially. Common deletion of an 8-cM region flanked by cMSR-32 and cC18-245, at 8p21.3-p22, suggested the presence of a tumor suppressor gene(s) in this interval. Correlation between allelic losses on 8p and clinicopathological parameters were examined in the 51 cases for which detailed clinical data were available; allelic losses on 8p were observed in none of five tumors at stage T1 (0%), nine of 20 tumors at stage T2 (45%), and 17 of 26 tumors at stage T3/T4 (65%). A higher frequency of allelic losses on 8p was observed in poorly differentiated tumors (10/14, 71%) than in well differentiated tumors (3/13, 23%). The association of allelic losses on 8p with advanced clinical stage and poor differentiation implies that loss or inactivation of a tumor suppressor gene(s) on 8p may play a role in the progression of HCC.

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Section: Southern Blot Hybridizationmentioning

confidence: 99%

Allelic loss at chromosome band 8p21.3‐p22 is associated with progression of hepatocellular carcinoma

Emi

Fujiwara

Ohata

et al. 1993

Genes Chromosomes & Cancer

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“…By colony hybridization with labeled total human DNA as a probe, we selected chromosome-specific cosmid clones and constructed high-resolution cytogenetic maps with the average distance between markers of 250 kb-1 Mb. A total of more than 3,300 genomic cosmid markers have so far been mapped on chromosomes 1, 2, 3, 5, 6, 8, 9, 11, 12, and 17 (Yamakawa et aL, 1991b;Hori et al, 1992;Takahashi et aL, , 1993aTakahashi et aL, , b, 1994aEmi et al, 1992;Inazawa et aL, 1993;Ariyama et al, 1995).…”

Section: Construction Of Chromosomal Mapsmentioning

confidence: 99%

Application of DNA markers to clinical genetics

Nakamura

1996

Jap J Human Genet

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SummaryDNA technology using DNA sequence polymorphisms has brought a new system to the fields of medicine and forensic science, especially for the studies of genetic diseases and tumor suppressor genes, and for identification of individuals for forensic purposes. Linkage analysis based on segregation of polymorphic alleles in affected families has contributed to identification of many genetic diseases. We isolated a large number of polymorphic DNA markers, called VNTR (variable number of tandem repeat) markers and identified the APC gene that is responsible for familial adenomatous polyposis (FAP) by means of a so-called "positional cloning" and characterized germline and somatic mutations of the APC gene in colorectal cancer patients. In addition, we have applied genetic information during colorectal carcinogenesis to sensitive diagnosis of lymph-node metastasis of colorectal cancer.

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“…Interspecies somatic cell hybrids containing a single human chromosome have been used as a donor of human chromosome specific DNA (Emi et al, 1992;Hori et al, 1992;Kugoh et a[., 1995). An irradiation and fusion procedure has reduced the size of human chromosomes in hybrid cells (Leach et al, 1989;Tamari et al, 1992;Gerhard et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Cosmids and transcribed sequences from chromosome 11q23

et al. 1995

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SummaryTo obtain cosmid markers and transcribed sequences from a specific chromosome region, a series of radiation-reduced hybrids (RHs) containing various regions of human chromosome ll was prepared from microcell hybrid A9 (neoll) cells containing a normal human chromosome 11 tagged with pSV2neo at llpll.2. Among 15 radiation hybrid clones isolated, RH(ll)-9 which contains a q23 fragment in addition to the neo integration site, was used for the construction of a cosmid library. Cosmid clones having human DNA sequences were screened, and localized by Southern hybridization with the radiation hybrid panel. Fifty-nine cosmids were assigned to 1lq23 and 6 cosmids to llpll.2. Exon amplification proceeded with 23 of the 59 cosmids and 16 putative exons were cloned. Three of them were identical to those constituting a known gene which locates on q23 (ATDC), and the others were unknown. Thus, the RHs containing various subchromosomal fragments of chromosome 11 were useful for constructing region-specific DNA markers. The RH-(11)-9 cells and putative exons also facilitate the positional cloning of genes in the 1 lq23 region.

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Isolation and mapping of 88 new RFLP markers on human chromosome 8

Cited by 20 publications

References 29 publications

Allelic loss at chromosome band 8p21.3‐p22 is associated with progression of hepatocellular carcinoma

Allelic loss at chromosome band 8p21.3‐p22 is associated with progression of hepatocellular carcinoma

Application of DNA markers to clinical genetics

Cosmids and transcribed sequences from chromosome 11q23

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